Detection of rotavirus in dogs with diarrhea in Brazil

Gabbay, Yvone Benchimol; Homem, Valéria Stacchini Ferreira; Munford, Veridiana; Alves, Antonia S.; Mascarenhas, Joana D. P.; Linhares, Alexandre C.; Rácz, Maria Lucia

doi:10.1590/s1517-83822003000100016

Cited by 10 publications

(5 citation statements)

References 18 publications

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“…Pets are continually exposed to infection by rotavirus, and yet that did not show clinical signs, they can host the virus in your intestinal epithelium, or carry the same through their snouts and paws and transmit it to other species likely, including human beings (Matthijnssens et al, 2011;Song e Hao, 2009). Dogs infected by RV may act as a reservoir and source of disseminating viruses, maintaining their spread in the environment (Gabbay et al, 2003;Tarsitano et al, 2010). These infections may also have some deleterious effects on the health status of free-living populations of pampas foxes and crab-eating foxes.…”

Section: Resultsmentioning

confidence: 99%

Adenoviruses of canine and human origins in stool samples from free-living pampas foxes (<italic>Lycalopex gymnocercus</italic>) and crab-eating foxes (<italic>Cerdocyon thous</italic>) in São Francisco de Paula, Rio dos Sinos basin

et al. 2015

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The spread of enteric viruses of domestic animals and human beings to wild species can be facilitated by the resistance of these viruses on the environment and their ability to be transmitted by water and contaminated food. The health status of the populations of pampas foxes (Lycalopex gymnocercus) and crab-eating foxes (Cerdocyon thous) is largely unknown and the landscapes occupied by these animals in southern Brazil have been threatened by human occupation and expansion of agriculture. In this work, the search of genomes of human and canine adenoviruses in feces from these wild carnivores was used to track the dissemination of domestic animals and human pathogens to the free-living populations in a wildlife reserve located in southern Brazil. This was performed by virus-specific differential real-time polymerase chain reactions (qPCR) on stool specimens, avoiding capture and additional stress to the animals. Genus-specific conventional reverse-transcriptase PCR (RT-PCR) was complementarily performed aiming the detection of enteroviruses (EV) and rotaviruses (RV) on these same samples. HAdV genomes were found on 14 out of the 17 (82.35%) stool samples analysed, whereas CAV was found co-infecting 5 of these samples. RV genomes were detected on 7 of the 17 samples (41.18%) and all samples were negative for EV. The results point to the dispersion of HAdV and RV at a high rate to these species of South American wild carnivores, which can be an effect of growing anthropisation of the habitat of these animals.Keywords: Lycalopex gymnocercus, Cerdocyon thous, adenovírus, rotavirus, enterovirus. Adenovírus de origens canina e humana em fezes de graxains (Lycalopex gymnocercus) e cachorros-do-mato (Cerdocyon thous) de vida livre em São Francisco de Paula, bacia do Rio dos Sinos ResumoA disseminação de vírus entéricos de animais domésticos e seres humanos para espécies selvagens pode ser facilitada pela resistência desses vírus no ambiente e sua capacidade de ser transmitida por água e alimentos contaminados. O estado de saúde das populações de Graxains-do-campo (Lycalopex gymnocercus) e Cachorros-do-mato (Cerdocyon thous) é em grande parte desconhecida e as paisagens ocupadas por estes animais no sul do Brasil têm sido ameaçadas pela ocupação humana e a expansão da agricultura. Neste trabalho, utilizou-se a pesquisa de genomas de adenovírus humanos (HAdV ) e caninos (CAV-1 e -2) em amostras fezes desses carnívoros selvagens com vistas a diagnosticar a disseminação de patógenos de animais domésticos e seres humanos às populações de vida livre em uma reserva de vida selvagem, localizado no sul do Brasil. Foram realizadas reações em cadeia da polimerase diferenciais e em tempo real (qPCR) de adenovírus específicos em amostras de fezes, evitando a captura e estresse adicional para os animais. PCRs gênero-específicas convencionais com transcrição reversa prévia (RT-PCR) foram ainda realizadas visando a detecção de enterovírus (EV) e rotavírus (RV) nestas mesmas amostras. Genomas de HAdV foram encontrados em 14 a 17...

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Section: Resultsmentioning

confidence: 99%

Adenoviruses of canine and human origins in stool samples from free-living pampas foxes (<italic>Lycalopex gymnocercus</italic>) and crab-eating foxes (<italic>Cerdocyon thous</italic>) in São Francisco de Paula, Rio dos Sinos basin

et al. 2015

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“…The existence of enteric viruses infecting animals seems to denote potential reservoirs for infections in humans and, consequently, the study of these viral pathogens in animals may be of key importance for a better understanding of the evolution of these agents, as well as in shedding light in the process of possible breaking down interspecies barrier 28,29,30,31 .…”

Section: Introductionmentioning

confidence: 99%

Group A rotavirus gastroenteritis in orphaned Leopardus tigrinus and Leopardus pardalis (Carnivora: Felidae) in the Eastern Amazon

Gabriel¹,

Pereira²,

Silva³

et al. 2023

Revista Pan-Amazônica de Saúde

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Rotaviruses are highly infectious and transmitted by the fecal-oral route, representing a leading cause of diarrheal deaths in children in developing countries. Additionally, it causes significant economic impacts as an enteropathogen among domestic animals. This report presents clinical and diagnostic findings in two cases of rotavirus (RV) infection involving orphaned Leopardus tigrinus (Schreber, 1775) and Leopardus pardalis (Linnaeus, 1758), representing the first registered group A RV (RVA) infection in these species. Both felids were rescued in Pará State, Amazon Region, by the Brazilian Institute of Environment and Renewable Natural Resources and treated in an intensive care ward in a public Environmental Park of Belém City. After adaptation up to the 40th day of the quarantine period, these animals had no typical symptoms of acute, fulminant gastroenteritis. The collected samples were examined at the Virology Laboratory of Instituto Evandro Chagas. RVA antigen was detected in the blood and fecal samples of L. tigrinus by enzyme-linked immunosorbent assay and immunochromatography. RVA-positive samples were subjected to polyacrylamide gel electrophoresis and reverse transcriptase polymerase chain reaction for VP4 and VP7 genes using conventional set primers but with negative results. Stools were examined for ova and parasites yielding negative results and for intestinal endoparasites, yielding negative results. The animals died within a few days following a clinical exacerbation, therefore unresponsive to treatment. Necropsies and histopathological analysis were performed at the Laboratory of Veterinary Pathology of Universidade Federal Rural da Amazônia. Despite the pathologic findings typical of RV infection hemorrhagic enteritis was unusual if presumed etiology is considered.

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“…The existence of enteric viruses in animals seems as a potential reservoir for infections in humans and, consequently, the study of these viruses in animals is considered the key to gaining a greater understanding of the evolution of these agents, as well as understanding the process of breaking down interspecies barrier [28,29,30,31].…”

Section: Introductionmentioning

confidence: 99%

Group A Rotavirus Gastroenteritis in Orphaned Leopardus Tigrinus and Leopardus Pardalis (CARNIVORA: FELIDAE) in the Eastern Amazon

Gabriel¹,

Pereira²,

Silva³

et al. 2020

Preprint

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Rotaviruses are highly infectious and typically transmitted by fecal-oral route via in the tropics and leading the cause of diarrheal deaths in children of developing countries, besides causing significant economic impacts like neonatal disease agents of domestic animals. This present report aims to present the clinical and diagnostic findings of two confirmed cases of rotavirus (RV) infection in orphaned Leopardus tigrinus (Schreber, 1775) and Leopardus pardalis (Linnaeus, 1758), the first register of the infection by group A rotavirus in these species. Both felids were rescued in the Pará State Amazon Brazil by the IBAMA (the Brazilian Institute of Environment and Renewable Natural Resources), and treated by veterinarians into intensive care ward in a public Environmental Park of Belém city. After the adaptation period to the quarantine, these animals showed non-specific symptoms of acute fulminant gastroenteritis. Rotavirus group A antigen was identified in blood and faecal samples of L. tigrinus analyzed by immunochromatography (ICG) and immunoassay methods (ELISA) at the Virology Laboratory of the Institute Evandro Chagas. The animals died within few days during the clinical exacerbation unresponsive to current treatment, its necropsies and histopathological analysis were performed in the Laboratory of Veterinary Pathology of the Federal Rural University of Amazonia (UFRA). Despite the compatible pathologic findings of rotavirus infection in both animals, the atypical hemorrhagic character was a curious finding, considering the presumed etiology.

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Detection of rotavirus in dogs with diarrhea in Brazil

Cited by 10 publications

References 18 publications

Adenoviruses of canine and human origins in stool samples from free-living pampas foxes (<italic>Lycalopex gymnocercus</italic>) and crab-eating foxes (<italic>Cerdocyon thous</italic>) in São Francisco de Paula, Rio dos Sinos basin

Adenoviruses of canine and human origins in stool samples from free-living pampas foxes (<italic>Lycalopex gymnocercus</italic>) and crab-eating foxes (<italic>Cerdocyon thous</italic>) in São Francisco de Paula, Rio dos Sinos basin

Group A rotavirus gastroenteritis in orphaned Leopardus tigrinus and Leopardus pardalis (Carnivora: Felidae) in the Eastern Amazon

Group A Rotavirus Gastroenteritis in Orphaned Leopardus Tigrinus and Leopardus Pardalis (CARNIVORA: FELIDAE) in the Eastern Amazon

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