DETECTION OF SAG1 AND BAG1 Toxoplasma gondii DNA PROBES LABELLED WITH DIGOXIGENIN-11-dUTP

Abstract

The objective of this research was to detect a minimum concentration of the probes that could be used for dot blot hybridization analysis. The method required labeled DNA probes. In this study a non-radioactive label of Digoxigenin-11-dUTP was used for labeling the Sag1 and the Bag1 of Toxoplasma gondii DNA probe. Labeling method for the probes was done according to the random primed labeling technique. The result showed that 0.67 pg/µl Sag1 probe and 0.58 pg/µl Bag1 probe could be detected by anti-Dig-antibody. It could be concluded that 0.67 pg/µl Sag1 probe and 0.58 pg/µl Bag1 probe could be used to diagnose toxoplasmosis by dot blot hybridization method.

Detection of Toxoplasma gondii Parasite on Blood Donor at PMI Kabupaten Magelang by Rapid Diagnostic Methods in 2022

“…In Indonesia, about 2-51% of the population has Toxoplasmosis infection which is divided into several regions. There are 58% in North Sulawesi, 10-12% in Jakarta, 9% in Surabaya, 62.5% in Central Java, 20% in Yogyakarta, 2% in Boyolali, 51% in Heavy Java, 9% in North Sumatra, 3% in West Kalimantan, 31% in South Kalimantan, 27% in Central Sulawesi, and 16% in Palu [5]. Transmission of toxoplasmosis can occur due to human behaviour such as the habit of keeping cats, eating raw vegetables and fruits that are less clean, the habit of not washing hands when eating, and consuming food and drinks served without lids that are potentially contaminated with oocysts as well as work related to soil such as gardening and farming [6,7,8].…”