Detection of salmonellae by using Rambach agar and by a C8 esterase spot test

Freydière, Anne-Marie; Gille, Y.

doi:10.1128/jcm.29.10.2357-2359.1991

Cited by 29 publications

(11 citation statements)

References 2 publications

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“…T h e sensitivities of the MUCAP, SM-ID, Rambach and Hektoen agar tests were 100, 93, 88 and 99%, respectively, and their specificities were 74, 97, 76 and 59%, respectively. Other authors have reported similarly high rates of sensitivity and specificity of these chromogenic media using pure Salmonella strains (Freydiere and Gille 1991 ;Grunewald et al 1991).…”

Section: Resultsmentioning

confidence: 65%

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Comparison of three rapid methods for identification of Salmonella spp

Manafi

Willinger

1994

Lett Appl Microbiol

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A study was carried out to compare three rapid methods for detection of Salmonella spp. The fluorogenic MUCAP test (Biolife, Italy), the SM‐ID agar test (bioMérieux, France) and the Rambach agar test (Merck, Germany) were used in this study to examine 103 strains (69 Salmonella strains and 34 non‐Salmonella strains). Two conventional culture media, Hektoen and Leifson agars, were also included. The sensitivities of the MUCAP, SM‐ID, Rambach and Hektoen agar tests for pure strains were 100, 93, 88 and 99%, respectively, and their specificities were 74, 97, 76 and 59%, respectively. A total of 100 stool samples from patients with acute diarrhoea was also tested and showed great discrepancy between the different methods. In agreement with other investigators, it was found that the discriminating capacity of Rambach and SM‐ID as primary plating media was very restricted. The MUCAP test was very sensitive, rapid and easy to perform but not very specific. In view of these results, it is essential to combine different methods for the accurate and reliable detection of Salmonella strains.

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Section: Resultsmentioning

confidence: 65%

“…(1991) and Davies and Wray (1994). Comparative studies using these media were carried out by Freydiere and Gille (1991), Manafi and Sommer (1992), and Dusch and Altwegg (1993).…”

Section: Introductionmentioning

confidence: 99%

Comparison of three rapid methods for identification of Salmonella spp

Manafi

Willinger

1994

Lett Appl Microbiol

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“…One method for identifying Salmonella spp. in the clinical laboratory involves the use of methylumbelliferyl caprylate (MUCAP), a substrate that fluoresces upon hydrolysis of the ester bond (1,8,11,22). This substrate can be used to distinguish salmonellae from other bacteria, including Escherichia, Enterobacter, Yersinia, and Shigella spp.…”

Section: Resultsmentioning

confidence: 99%

The apeE Gene of Salmonella typhimurium Encodes an Outer Membrane Esterase Not Present in Escherichia coli

et al. 1998

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Salmonella typhimurium apeR mutations lead to overproduction of an outer membrane-associated N-acetyl phenylalanine β-naphthyl ester-cleaving esterase that is encoded by the apeE gene (P. Collin-Osdoby and C. G. Miller, Mol. Gen. Genet. 243:674–680, 1994). This paper reports the cloning and nucleotide sequencing of the S. typhimurium apeE gene as well as some properties of the esterase that it encodes. The predicted product of apeE is a 69.9-kDa protein which is processed to a 67-kDa species by removal of a signal peptide. The predicted amino acid sequence of ApeE indicates that it is a member of the GDSL family of serine esterases/lipases. It is most similar to a lipase excreted by the entomopathogenic bacterium Photorhabdus luminescens. The Salmonella esterase catalyzes the hydrolysis of a variety of fatty acid naphthyl esters and of C6 to C16 fatty acid p-nitrophenyl esters but will not hydrolyze peptide bonds. A rapid diagnostic test reported to be useful in distinguishing Salmonella spp. from related organisms makes use of the ability of Salmonella to hydrolyze the chromogenic ester substrate methyl umbelliferyl caprylate. We report that the apeE gene product is the enzyme in Salmonella uniquely responsible for the hydrolysis of this substrate. Southern blot analysis indicates thatEscherichia coli K-12 does not contain a close analog ofapeE, and it appears that the apeE gene is contained in a region of DNA present in Salmonella but not in E. coli.

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“…The appearance of blue fluorescence under UV light within 1 min of application was observed for all Salmonella test strains; however, colonies of some Pseudomonas and Acinetobacter spp. also fluoresced (6). Thus, although the sensitivity of the MUCAP test was high (100%), the specificity was low (80%), especially compared to that of a latex slide agglutination test (96%) (9).…”

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confidence: 94%

A Novel Chromogenic Ester Agar Medium for Detection of Salmonellae

Cooke¹,

Milès

Price

et al. 1999

Appl Environ Microbiol

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A novel agar medium, chromogenic Salmonella esterase (CSE) agar, for the differentiation of salmonellae is described. The agar contains peptones and nutrient extracts together with the following (grams per liter unless otherwise specified): 4-[2-(4-octanoyloxy-3,5-dimethoxyphenyl)-vinyl]-quinolinium-1-(propan-3-yl carboxylic acid) bromide (SLPA-octanoate; bromide form), 0.3223; lactose, 14.65; trisodium citrate dihydrate, 0.5; Tween 20, 3.0; ethyl 4-dimethylaminobenzoate, 0.035% (wt/vol), novobiocin, 70 mg liter−1. The key component of the medium is SLPA-octanoate, a newly synthesized ester formed from a C8fatty acid and a phenolic chromophore. In CSE agar, the ester is hydrolyzed by Salmonella spp. to yield a brightly colored phenol which remains tightly bound within colonies. After 24 h of incubation at 37 or 42°C, colonies of typical Salmonellaspp. were burgundy colored on a transparent yellow background, whereas non-Salmonella spp. were white, cream, yellow or transparent. CSE agar was evaluated by using a panel of strains including a high proportion of Salmonella and non-Salmonella strains giving atypical reactions on other differential agars. The sensitivity (93.1%) of CSE agar for non-typhi salmonellae compared favorably with those of Rambach (82.8%), xylose-lysine-deoxycholate (XLD; 91.4%), Hektoen-enteric (89.7%), and SM ID (91.4%) agars. The specificity (93.9%) was also comparable to those of other Salmonellamedia (SM ID agar, 95.9%; Rambach agar, 91.8%; XLD agar, 91.8%; Hektoen-enteric agar, 87.8%). Strains of Citrobacter freundii and Proteus spp. giving false-positive reactions with other media gave a negative color reaction on CSE agar. CSE agar enabled the detection of >30 Salmonellaserotypes, including agona, anatum,enteritidis, hadar, heidelberg,infantis, montevideo, thompson,typhimurium, and virchow, which accounted for 91.8% of the salmonella isolates recorded by the Public Health Laboratory Service (Colindale, London, England) for 1997.

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Detection of salmonellae by using Rambach agar and by a C8 esterase spot test

Cited by 29 publications

References 2 publications

Comparison of three rapid methods for identification of Salmonella spp

Comparison of three rapid methods for identification of Salmonella spp

The apeE Gene of Salmonella typhimurium Encodes an Outer Membrane Esterase Not Present in Escherichia coli

A Novel Chromogenic Ester Agar Medium for Detection of Salmonellae

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