Detection of SARS-CoV-2 with SHERLOCK One-Pot Testing

Amplification-free detection of SARS-CoV-2 with CRISPR-Cas13a and mobile phone microscopy

“…Additional work will be necessary to fully translate this work to a widely available point-of-care device. First, while the current study used a lab-based RNA extraction step, an extraction-free protocol for sample preparation ( Arizti-Sanz et al., 2020 ; Joung et al., 2020a ; Myhrvold et al., 2018 ) will be necessary to minimize the number of steps in the assay and the overall sample-to-answer turnaround time. Second, while we chose to integrate a mobile phone into our detection device as a way of rapidly demonstrating what mass-produced electronics could achieve, other configurations of an image-based diagnostic, including use of an embedded sensor that wirelessly connects to a mobile phone, could be built at scale with at least comparable performance.…”

PfAgo-based detection of SARS-CoV-2

“…Even though RT-qPCR test is rapid, specific and economic, it takes hours to perform and requires specialized reagents, expensive equipment and skilled operators ( Li et al, 2019 ). To overcome these limitations, alternative nucleic acid detecting methods, especially programmable endonuclease based methods were innovated ( Ding et al, 2020 ) ( Guo et al, 2020 ) ( Hou et al, 2020 ) ( Joung et al, 2020 ) ( Lucia et al, 2020 ).…”

Evaluating ELISA, Immunofluorescence, and Lateral Flow Assay for SARS-CoV-2 Serologic Assays

“…This Coronavirus Disease 2019 ) displays a benign course in most subjects, but may cause pneumonia, acute respiratory distress syndrome (ARDS) and death in an estimated 5-10% of patients (Zhang et al, 2020;Zhou et al, 2020). Currently, the gold standard for CoVID-19 diagnosis is the SARS-CoV-2 RT-PCR, despite new molecular methods relying on LAMP (Park et al, 2020) or CRISPR-Cas9 technologies (Joung et al, 2020). Antigenic tests of viral proteins are cheaper and faster than molecular diagnosis but their sensitivity is low (Scohy et al, 2020).…”