PfAgo-based detection of SARS-CoV-2

Wang, Fei; Yang, Jun; He, Ruyi; Yu, Xijie; Chen, Shuliang; Liu, Yang; Wang, Longyu; Li, Aitao; Liu, Linlin; Zhai, Chao; Ma, Lixin

doi:10.1016/j.bios.2020.112932

Cited by 86 publications

(65 citation statements)

References 24 publications

(19 reference statements)

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“…Therefore, the LOD of the presented sensor was found to be 39.5 fM. Table 2 showed the LOD for several COVID-19 sensors, which indicated although some of the previously reported sensors showed a lower LOD compared with our sensor; however, the present sensor has a good LOD besides its easy-to-use and fast response advantages [40] , [41] , [42] , [43] , [44] , [45] , [46] , [47] , [48] , [49] , [50] .…”

Section: Resultsmentioning

confidence: 62%

Synthesis of gold nanoparticles@reduced porous graphene-modified ITO electrode for spectroelectrochemical detection of SARS-CoV-2 spike protein

El‐Said

Al‐Bogami

Alshitari

2022

Spectrochimica Acta Part A: Molecular and Biomolecular Spectros

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Section: Resultsmentioning

confidence: 62%

Synthesis of gold nanoparticles@reduced porous graphene-modified ITO electrode for spectroelectrochemical detection of SARS-CoV-2 spike protein

El‐Said

Al‐Bogami

Alshitari

2022

Spectrochimica Acta Part A: Molecular and Biomolecular Spectros

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“…Furthermore, the CRISPR-Cas13-based detection technique can distinguish SARS-CoV-2 and its mutants (Wang et al, 2021). Recently, Wang et al (2020) developed a highly sensitive, much more specific and accurate PfAgo-based detection of SARS-CoV-2, which can also distinguish mutants of coronavirus by combining the programmable nuclease PfAGO with RT-PCR technology (Wang et al, 2020).…”

Section: Synthetic Biology Involved In Pathogen Identification and Vaccine Developmentmentioning

confidence: 99%

Advances in Synthetic Biology and Biosafety Governance

Zhao

Zheng

et al. 2021

Front. Bioeng. Biotechnol.

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Tremendous advances in the field of synthetic biology have been witnessed in multiple areas including life sciences, industrial development, and environmental bio-remediation. However, due to the limitations of human understanding in the code of life, any possible intended or unintended uses of synthetic biology, and other unknown reasons, the development and application of this technology has raised concerns over biosafety, biosecurity, and even cyberbiosecurity that they may expose public health and the environment to unknown hazards. Over the past decades, some countries in Europe, America, and Asia have enacted laws and regulations to control the application of synthetic biology techniques in basic and applied research and this has resulted in some benefits. The outbreak of the COVID-19 caused by novel coronavirus SARS-CoV-2 and various speculations about the origin of this virus have attracted more attention on bio-risk concerns of synthetic biology because of its potential power and uncertainty in the synthesis and engineering of living organisms. Therefore, it is crucial to scrutinize the control measures put in place to ensure appropriate use, promote the development of synthetic biology, and strengthen the governance of pathogen-related research, although the true origin of coronavirus remains hotly debated and unresolved. This article reviews the recent progress made in the field of synthetic biology and combs laws and regulations in governing bio-risk issues. We emphasize the urgent need for legislative and regulatory constraints and oversight to address the biological risks of synthetic biology.

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“…The programmable binding and cleavage activities of pAgos are similar to that of the CRISPR-Cas system, which means pAgos can potentially be used in genome editing applications (9), molecular cloning (24), and nucleic acid detection (25)(26)(27)(28). Though eAgos prefer to use small RNA guides to cleave RNA targets, there are only a few pAgos that have been reported to recognize RNA targets, and no pAgos with the ability to preferentially cleave RNA targets with high accuracies at mesophilic temperatures have been described to date.…”

Section: Introductionmentioning

confidence: 99%

A programmable pAgo nuclease with RNA target preference from the psychrotolerant bacteria Mucilaginibacter paludis

Wang

et al. 2021

Preprint

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Argonaute (Ago) proteins are programmable nuclease found in both eukaryotes and prokaryotes. Prokaryotic Argonaute proteins (pAgos) share a high degree of structural homology with eukaryotic Argonaute proteins (eAgos) and eAgos are considered to evolve from pAgos. However, the majority of studied pAgos prefer to cleave DNA targets, and eAgos exclusively cleave RNA targets. Here, we characterize a novel pAgo, MbpAgo, from psychrotolerant bacteria Mucilaginibacter paludis that can be programmed with DNA guides and prefers to cleave RNA targets rather than DNA targets. MbpAgo can be active at a wide range of temperatures (4-65°C). In comparison with previously studied pAgos, MbpAgo is able to utilize 16-nt long 5'phosphorylated and 5'hydroxylated DNA guides for efficient and precise cleavage and displays no obvious preference for the 5'end nucleotide of a guide. Furthermore, the cleavage efficiency can be regulated by mismatches in the central and 3'supplementary regions of the guide. MbpAgo can efficiently cleave highly-structured RNA targets using both 5'phosphorylated and 5'hydroxylated DNA guides in the presence of Mg2+ or Mn2+. In conclusion, we have demonstrated that MbpAgo is a unique programmable nuclease that has a strong preference for RNA targets, with great potential applications in the field of nucleic acid biotechnology.

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PfAgo-based detection of SARS-CoV-2

Cited by 86 publications

References 24 publications

Synthesis of gold nanoparticles@reduced porous graphene-modified ITO electrode for spectroelectrochemical detection of SARS-CoV-2 spike protein

Synthesis of gold nanoparticles@reduced porous graphene-modified ITO electrode for spectroelectrochemical detection of SARS-CoV-2 spike protein

Advances in Synthetic Biology and Biosafety Governance

A programmable pAgo nuclease with RNA target preference from the psychrotolerant bacteria Mucilaginibacter paludis

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