Detection of Serratia Outbreaks in Hospital

Farmer, J. J.; Davis, BettyR.; Hickman, FrancisW.; Presley, D.B.; Gp, Bodey; Neguţ, M; Bobo, Raymond A.

doi:10.1016/s0140-6736(76)92539-3

Cited by 79 publications

(35 citation statements)

References 20 publications

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“…The nucleotide sequence of smfA, the gene encoding the major structural component of MR fimbriae, revealed that this gene encodes a 174-amino-acid polypeptide with a typical procaryotic signal peptide. The primary structure of the smfA product showed significant homology with the primary structure of the E. coli fimbrial subunit.Serratia marcescens is a bacterial species causing nosocomial infection in the urinary and respiratory tracts (3,16,17,30). Gram-negative bacteria isolated from patients with an urinary tract infection adhere to uroepithelial surfaces by bacterial surface appendages known as pili or fimbriae (13,15,43).…”

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Cloning and sequence of the gene encoding the major structural component of mannose-resistant fimbriae of Serratia marcescens

et al. 1988

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Serratia marcescens US46, a human urinary tract isolate, exhibits mannose-resistant hemagglutination and agglutinates yeast cells, thereby indicating that it has two types of adhesins. We constructed a cosmid library for the DNA of this organism and isolated DNA clones carrying genes for mannose-sensitive (MS) and mannose-resistant (MR) fimbriae. On introduction of the cloned genes into Escherichia coli K-12, MS and MR fimbriae were formed. These fimbriae were functionally and morphologically indistinguishable from those of S. marcescens. Subcloning of these gene clusters revealed that the genes encoding MS fimbriae reside on a 9-kilobase (kb) DNA fragment, while those encoding MR fimbriae are present on a 12-kb fragment. Transposon insertion and maxicell analyses revealed thgt formation of MR fimbriae is controlled by several genes which reside on the 9-kb fragment. The nucleotide sequence of smfA, the gene encoding the major structural component of MR fimbriae, revealed that this gene encodes a 174-amino-acid polypeptide with a typical procaryotic signal peptide. The primary structure of the smfA product showed significant homology with the primary structure of the E. coli fimbrial subunit.Serratia marcescens is a bacterial species causing nosocomial infection in the urinary and respiratory tracts (3,16,17,30). Gram-negative bacteria isolated from patients with an urinary tract infection adhere to uroepithelial surfaces by bacterial surface appendages known as pili or fimbriae (13,15,43). S. marcescens isolated from such patients also possesses fimbriae and adheres to uroepithelial cells (47).There are at least two classes of adhesins in S. marcescens (1,26,27). One class, designated MRHA, agglutinates chicken erythrocytes in the presence of D-mannose and is associated with thick rigid fimbriae. The other, named MSHA, exhibits mannose-sensitive hemagglutination of guinea pig and chicken erythrocytes and is associated with thin flexible fimbriae. The latter adhesin is also responsible for agglutinating yeast cells. S. marcescens US46, isolated from a patient with a urinary tract infection, exhibits mannose-resistant hemagglutination and agglutinates yeast cells, thereby indicating that this strain possesses two classes of adhesins (26).To elucidate mechanisms related to the genetic control of formation of mannose-resistant (MR) and mannose-sensitive (MS) fimbriae of S. marcescens, we attempted to clone the DNA fragments carrying these genes. We placed fragments of the chromosomal DNA of strain US46 on appropriate vectors and introduced them into the nonfimbriated strain E. coli K-12. Thus, we were able to clone the chromosomal determinants for MR and MS fimbriae of S. marcescens US46. We determined the nucleotide sequence of the smfA (for S. marcescens fimbria A) gene, which encodes the major structural component (major fimbrial subunit) of MR fimbriae of S. marcescens US46. MATERIALS AND METHODSBacterial strains and growth conditions. The bacterial strains used are listed in Table 1. E. coli K-12, strain * Cor...

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“…Serratia marcescens is a bacterial species causing nosocomial infection in the urinary and respiratory tracts (3,16,17,30). Gram-negative bacteria isolated from patients with an urinary tract infection adhere to uroepithelial surfaces by bacterial surface appendages known as pili or fimbriae (13,15,43).…”

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confidence: 99%

Cloning and sequence of the gene encoding the major structural component of mannose-resistant fimbriae of Serratia marcescens

et al. 1988

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“…There are, however, two reported cases of septicemia arising from transfusion of plasma. One was attributed to contamination of the water bath used for thawing (25); the other was reported to involve Serratia marcescens (40).…”

Section: Plasmamentioning

confidence: 99%

Transfusion-related bacterial sepsis

Wagner¹

1997

Current Opinion in Hematology

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“…Serratia marcescens was long considered to be nonpathogenic, but it is now known to be responsible for outbreaks of nosocomial infection (5,6,11). Urinary and respiratory tract infections are the main diseases from which S. marcescens is frequently isolated.…”

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“…Patients with significant Serratia infection have a variety of debilitating diseases such as diabetes mellitus, heart disease, renal insufficiency or malignant diseases. In addition, long-term treatment with antibiotics and the presence of an in-dwelling urethral catheter are thought to be associated with Serratia infection (5,6,8,10).…”

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Pili Mediated Agglutination of Serratia marcescens in Human Urine

Amako

Ohshima

Yasunaka

et al. 1981

Microbiology and Immunology

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Of 51 strains of Serratia marcescens isolated from patients with urinary or respiratory tract infections, 35 agglutinated in human urine. The agglutinating strains possessed numerous pili which were morphologically distinct from common pili or type I pili. The diameter of the pili was 3 nm and the average length was 0.3 um. Electron microscopic examination showed that 80% or more of the cells of the agglutinating strains and°to 8% of the cells of the nonagglutinating strains were piliated. When an agglutinating strain was heated at 55 C for 10 min, it lost its agglutinating capacity and concomitantly its pili. These results suggest that the agglutination might occur because of interactions between the pili and some factors in human urine. The urinary slime appears to contain these agglutinating factors.

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Detection of Serratia Outbreaks in Hospital

Cited by 79 publications

References 20 publications

Cloning and sequence of the gene encoding the major structural component of mannose-resistant fimbriae of Serratia marcescens

Cloning and sequence of the gene encoding the major structural component of mannose-resistant fimbriae of Serratia marcescens

Transfusion-related bacterial sepsis

Pili Mediated Agglutination of Serratia marcescens in Human Urine

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