Detection of Serum Anti-ganglioside Antibodies by Latex Agglutination Assay in Guillain-Barre Syndrome: Comparison with ELISA

Irie, Seiji; Saito, Toyokazu; Kanazawa, Naomi; Ogino, Mieko; Ogino, Yutaka; Sakai, Fumihiko

doi:10.2169/internalmedicine.42.490

Cited by 3 publications

(3 citation statements)

References 33 publications

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“…Besides, the profile of different ganglioside autoantibodies in GBS was reported to vary depending upon the technique used. [ 29 30 31 32 ] Various studies have employed different types of immunoassays in profiling antiganglioside autoantibodies. Therefore, more precise information is required to explain the methodical and ethnic differences in anti-ganglioside and anti-GSC antibodies in GBS patients.…”

Section: Discussionmentioning

confidence: 99%

Impact of Antecedent Infections on the Antibodies against Gangliosides and Ganglioside Complexes in Guillain-Barré Syndrome

Nagappa¹,

Dutta²,

Debnath³

et al. 2022

Annals of Indian Academy of Neurology

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Background and Aims: Guillain-Barré Syndrome (GBS), an immune-mediated neuropathy, is characterized by antibodies against gangliosides/ganglioside complexes (GSCs) of peripheral nerves. Antecedent infections have been reported to induce antibodies that cross-react with the host gangliosides and thereby have a pivotal role in conferring an increased risk for developing GBS. Data pertaining to the impact of various antecedent infections, particularly those prevalent in tropical countries like India on the ganglioside/GSC antibodies is sparse. We aimed at exploring the association between six antecedent infections and the profile of ganglioside/GSC antibodies in GBS. Methods: Patients with GBS (n = 150) and healthy controls (n = 50) were examined for the serum profile of antibodies against GM1, GM2, GD1a, GD1b, GT1b, and GQ1b and their GSCs by ELISA. These antibodies were correlated with immunoreactivities against Campylobacter jejuni , Japanese encephalitis (JE), dengue, influenza, zika, and chikungunya infections. Results: The frequencies of antibodies against six single gangliosides ( P < 0.001) and their GSCs ( P = 0.039) were significantly higher in patients as compared to controls. Except for GT1b-antibody which was more frequent in axonal GBS, none of the other ganglioside/GSC antibodies correlated with the electrophysiological subtypes of GBS. Antecedent JE infection was significantly associated with increased frequency of antibodies against GD1a, GD1b, GT1b, and GQ1b. Antibodies against GSCs were not influenced by the antecedent infections. Interpretation: This study for the first time shows an association between antecedent JE infection and ganglioside antibodies in GBS. This finding reinforces the determining role of antecedent infections on ganglioside antibody responses and the subsequent immunological processes in GBS.

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Section: Discussionmentioning

confidence: 99%

Impact of Antecedent Infections on the Antibodies against Gangliosides and Ganglioside Complexes in Guillain-Barré Syndrome

Nagappa¹,

Dutta²,

Debnath³

et al. 2022

Annals of Indian Academy of Neurology

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“…Other methods include line immunoassay, agglutination immunoassay, and glycol-array (12), and so on. The positive rate for agglutination immunoassay was 24% (13). Thin layer chromatography is considered to be the gold standard, but it is often unavailable for routine diagnostics (14).…”

Section: Discussionmentioning

confidence: 99%

Anti-ganglioside Antibodies in Guillain-Barre Syndrome: A Novel Immunoblotting-Panel Assay

Zhu

Zhang

et al. 2021

Front. Neurol.

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Objective: This study aimed to determine the diagnostic efficiency of a novel immunoblotting detection assay for anti-ganglioside antibodies (AGAs) in the Guillain–Barre syndrome (GBS).Method: Serum immunoglobulin (IgG and IgM) of AGAs were measured in 121 participants from a registered cohort study of immune-mediated neuropathies and 29 healthy controls by immunoblotting panel assay. Sensitivity, specificity, and positive predictive value (PPV) of the assay were compared to calculate the diagnostic accuracy.Result: In our cohort, any of the AGAs were positive in 42.4% of the GBS patients. The sensitivity and specificity of AGAs (both IgG and IgM) in the diagnosis of GSB were 42 and 76% while for IgG-AGAs were 35 and 87%. AGAs positivity had a significant association with the AMAN subtype (P = 0.0004), and the sensitivity, specificity of AGAs in AMAN were 86, 69%, respectively with high (AUC = 0.78, p = 0.002) discriminative powers. GM1-IgG AGA was more common and specific to AMAN patients than other GBS forms (p = 0.008).Conclusion: Our novel immunoblotting detection assay could complement GBS diagnosis. IgG-AGAs were more likely to be detected in GBS, and GM1-IgG AGA could assist AMAN diagnosis.

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“…Alaedini and Latov reported the latex agglutination assay for detection of anti-ganglioside antibodies (15). In this issue, Irie et al reported the results of antiganglioside antibody assay by the use of the above method with some modifications and compared the results with those of ELISA (16).…”

mentioning

confidence: 99%

Anti-ganglioside Antibodies in Guillain-Barré Syndrome; Useful Diagnostic Markers as Well as Possible Pathogenetic Factors

Kusunoki

2003

Intern. Med.

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Detection of Serum Anti-ganglioside Antibodies by Latex Agglutination Assay in Guillain-Barre Syndrome: Comparison with ELISA

Cited by 3 publications

References 33 publications

Impact of Antecedent Infections on the Antibodies against Gangliosides and Ganglioside Complexes in Guillain-Barré Syndrome

Impact of Antecedent Infections on the Antibodies against Gangliosides and Ganglioside Complexes in Guillain-Barré Syndrome

Anti-ganglioside Antibodies in Guillain-Barre Syndrome: A Novel Immunoblotting-Panel Assay

Anti-ganglioside Antibodies in Guillain-Barré Syndrome; Useful Diagnostic Markers as Well as Possible Pathogenetic Factors

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