Detection of SHV Gene from Extended-Spectrum Beta-Lactamases (ESBLs) Producing Isolates in a Tertiary Care Hospital

Saha, Mili Rani; Jhora, Sanya Tahmina; Khan, Tarek Mahbub; Paul, SK; Chowdhury, Durdana

doi:10.3329/bjmm.v8i2.31093

Cited by 3 publications

(2 citation statements)

References 13 publications

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“…In this study, K. pneumoniae was the most frequent ESBL producer, Klebsiella ESBL producer was 55.5% that similarity with Al-gamy et al [22] showed that ESBL production was 55% isolates of K. pneumoniae isolated from hospital-acquired infections in Riyadh. However, these contrast with Saha et al, [23] ESBL producers were observed among Klebsiella spp. (75%) and with AL-Subol and Youssef, [24] they were detected (67.5%) of K. pneumoniae, different rates for the prevalence of ESBL-producing K. pneumoniae are detecting in the variation difficult to explain but may be due to differences in antibiotic exhaustion and a variety of specimens handling time.…”

Section: Discussioncontrasting

confidence: 60%

Molecular Detection and Sequencing of SHV Gene Encoding for Extended-Spectrum Β-Lactamases Produced by Multidrug Resistance Some of the Gram-Negative Bacteria

Al-Ouqaili¹

2018

IJGP

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Background and Objective:The increase in extended-spectrum β-lactamases (ESBLs) producing microbes in recent years has led to the great challenge for the clinician in the treatment. The study aims to investigate the molecular basis of ESBL encoding-resistant gene, SHV to modern β-lactams. Also, to detect gene sequencing and to compare their genetic relatedness utilizing phylogenetic analysis. Patients and Methods: A total of 100 clinical isolates are submitting after identification to phenotypic confirmatory double disk synergy technique. Polymerase chain reaction was performed for the determination of SHV gene, and sequencing analysis for the amplified gene is also achieved. Results: A total of 27 isolates, 3 (11.1%), 15 (55.55%), and 9 (33.0%) of Escherichia coli, Klebsiella pneumoniae, and Pseudomonas aeruginosa were positive for a phenotypic and confirmatory test for ESBLs, respectively. SHV gene was detected only in 5 (31.25%) isolates of P. aeruginosa, 2 (12.5 %) E. coli, and 9 (56.25%) in K. pneumoniae. Conclusions: SHV gene plays an essential role in the resistance of ESBL producer isolates to new β-lactams. The sequencing of this gene revealed 98-99% compatibility range with the global standard gene in National Center of Biotechnology Information.

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Section: Discussioncontrasting

confidence: 60%

Molecular Detection and Sequencing of SHV Gene Encoding for Extended-Spectrum Β-Lactamases Produced by Multidrug Resistance Some of the Gram-Negative Bacteria

Al-Ouqaili¹

2018

IJGP

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“…These results are similar to the results of the study of nosocomial Gram-negative bacilli isolates by Singh et al (2012) and Mshana, (2009) who found ESBLs producer was 27% and 29%, respectively. These results were lower than the results conducted by Saha et al (2017) were registered 41.40% of the Gram-negative bacilli isolated from nosocomial infections. The use of clavulanic acid to inhibit an ESBL may induce high level expression of the chromosomal AmpC enzyme and may then antagonize rather than protect the antibacterial activity of the partner lactam, preventing the synergistic effect required to detect ESBL production, which may explain the lower percentage in this method (Apfalter et al, 2007).…”

Section: Discussioncontrasting

confidence: 78%

Phenotypic detection of extended spectrum beta-lactamases (ESBLs) in Gram-negative bacilli isolated from deferent clinical source

Sahel¹,

Abid²

2022

UTJsci

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This study aimed to the detection of extended spectrum beta-lactamases by phenotypic and genotypic methods in nosocomial Gram-negative isolates. A number of 194 samples from hospitalized patients (urine, burn swab, surgical swab, and sputum) were collected These patients were at Al-Hussein and Al-Nasiriyah teaching hospitals in Al-Nasiriyah city. ESBLs enzymes were detected phenotypically by ceftazidime (30 ?g) and cefotaxime (30 ?g) (Screening test) and Double Disk Synergy Test (DDST) (Confirming test). The results showed that out of 109 isolates 89 (81.65%) were Gram negative bacilli. A total of 68 Gram-negative were ?-lactamases producer. Moreover, the results of screening test recorded that 55 (80.88%) of isolates were resistant to cefotaxime and ceftazidime, while 17 (25%) isolates produced ESBLs by (DDST).

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Extended-spectrum beta-lactamase in Escherichia coli isolated from humans, animals, and environments in Bangladesh: A One Health perspective systematic review and meta-analysis

Islam

Rahman²,

Hassan

et al. 2023

One Health

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Detection of SHV Gene from Extended-Spectrum Beta-Lactamases (ESBLs) Producing Isolates in a Tertiary Care Hospital

Cited by 3 publications

References 13 publications

Molecular Detection and Sequencing of SHV Gene Encoding for Extended-Spectrum Β-Lactamases Produced by Multidrug Resistance Some of the Gram-Negative Bacteria

Molecular Detection and Sequencing of SHV Gene Encoding for Extended-Spectrum Β-Lactamases Produced by Multidrug Resistance Some of the Gram-Negative Bacteria

Phenotypic detection of extended spectrum beta-lactamases (ESBLs) in Gram-negative bacilli isolated from deferent clinical source

Extended-spectrum beta-lactamase in Escherichia coli isolated from humans, animals, and environments in Bangladesh: A One Health perspective systematic review and meta-analysis

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