Covid19 is an acute respiratory disease which is caused by a novel coronavirus. This virus has been found in January 7, 2020 from China. The virus was previously known as 2019-nCOV. This particular virus is high chance of spread. Preventive measures are very essential for the health care workers. Proper preventive measures can only be applied to stop the spread of this virus. Bangladesh Journal of Infectious Diseases, April 2020;7(suppl_1):S41-S44

Most ESBL Extended-Spectrum beta-lactamases (ESBLs) producing isolates carry TEM and SHV type betalactamases. Among these two types beta-lactamases SHV type was reported nosocomial infection outbreak in many countries in the world. The goal of this study was to see the rate of ESBLs production and also to detect SHV gene in those isolates recovered from a tertiary care hospital.

The aim of this study was to evaluate the efficacy of MRSA Chrome agar to detect methicillin resistant Staphylococcus aureus (MRSA) and compare it with 1µg oxacillin disc diffusion tests and detection of mecA gene by PCR. A total 116 Staphylococcus aureus (S. aureus), isolated from various clinical samples, were obtained from three tertiary care hospitals of Dhaka city. S. aureus was identified by colony characters, Gram stain and standard biochemical procedures. MRSA was detected by susceptibility to 1µg oxacillin disc, growth of denim blue color colonies of S. aureus on the Brilliance MRSA Chrome agar at 24 and 48 hours of incubation. PCR was performed for amplification of mecA gene as a gold standard method. Out of 116 isolated S. aureus, 33 (28.44%) were MRSA by oxacillin disc diffusion test where mecA gene was detected in 28 strains. On MRSA Chrome agar, 29 (25.0%) S. aureus produced denim blue colonies at 24 hours, of which 28 isolates possessed mecA gene. At 48 hours incubation, an additional 4 isolates yielded denim blue colonies from which mecA gene could not be identified. All the strains of S. aureus that produced denim blue colonies at 24 and 48 hours were resistant to oxacillin. The sensitivity, specificity and accuracy of oxacillin disc diffusion test were 100%, 94.31% and 95.68% and Chrome agar at 24 hours were 100%, 98.86% and 99.13% respectively. Thus MRSA Chrome agar could be good choice in clinical microbiology laboratory for rapid and accurate identification of MRSA.Ibrahim Med. Coll. J. 2013; 7(1): 1-4

This study has been undertaken to investigate the isolation and identification of EPEC strains from paediatric diarrhoeal patients.Total 300 samples were studied.Two hundred and seventy two samples from patients with diarrhoea and  28 samples from control children were collected from two tertiary care hospital. Esch. coli was isolated and identified from all the 300 samples including patient and control using standard microbiological techniques. EPEC strains were identified on the basis of  presence of bundle forming pilus (bfpA) gene. Out of 272 samples from diarrhoeal patient only Esch. coli was isolated from 240 (88.2%) samples. Shigella spp. with Esch. coli were isolated from 27 (10%) specimens and Salmonella spp. with Esch. coli were isolated from 5 (1.8%) samples. Among 272 samples 20 (7.35%) isolates were identified as EPEC on the basis of presence of bfpA gene detected by polymerase chain reaction. EPEC strains were identified from those 240 samples, from which Esch. coli had been isolated only. No EPEC strain was identified  from control children. Rapid and reliable detection of EPEC is required for successful microbiological surveillance and for treatment of EPEC mediated diarrhoeal disease. bfpA gene detection by polymerase chain reaction can be a appropriate method where facilities for polymerase chain reaction are available.DOI: http://dx.doi.org/10.3329/bjmm.v7i2.19324 Bangladesh J Med Microbiol 2013; 07(02): 2-5

Background: Wound infection is a major health problem that results in prolong hospital stay, increased treatment cost and are responsible for significant mortality and morbidity worldwide. The aim of the present study was to isolate and identify the bacterial pathogens causing wound infection and to determine their antimicrobial susceptibility profile.Methods: This cross sectional study was conducted at the Department of Microbiology, Sir Salimullah Medical College, Dhaka from January 2016 to December 2016. Wound swab samples were collected and inoculated into appropriate media. The bacterial pathogens were identified by using standard microbiological methods. Antimicrobial susceptibility test were performed using disc diffusion technique following Kirby-Bauer method.Results: Out of 239 wound swab samples analyzed 173 (72.4%) were culture positive. Majority (35.3%) of culture positive cases were in age group 16-30 years and 60.1% were male. Staphylococcus aureus (36.9%) was the predominant isolate, followed by Escherichia coli (35.8%), Pseudomonas spp. (17.3%) and Proteus spp. (5.8%). Bacterial isolates were highly resistant Amoxicillin (89-100%), Cephalosporin (60-100%), Ciprofloxacin (53-71%), while they were least resistant to Imipenem (0-14%) and Amikacin (17-30%).  Conclusions: In the present study most of the isolates showed high rate of resistance to commonly used antibiotics. Therefore regular monitoring and rational use of antibiotic should be practiced.