Detection of single-base mismatch at distal end of DNA duplex by electrochemical impedance spectroscopy

Ito, Toshiyuki; Hosokawa, Kazuo; Maeda, Mizuo

doi:10.1016/j.bios.2006.08.008

Cited by 54 publications

(41 citation statements)

References 17 publications

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“…Nevertheless, other investigators have reported contrasting results, i.e., a decrease in R CT after hybridization [27][28][29][30][31]. According to these groups, steric repulsion of the surface-bound DNA is the principal barrier between marker ions and the surface, decreasing this effect by duplex formation.…”

Section: Effect Of Ionic Strength Using 6-mercapto-1-hexanolmentioning

confidence: 86%

Effects of Ionic Strength and Probe DNA Length on the Electrochemical Impedance Spectroscopic Response of Biosensors

et al. 2010

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Impedance spectroscopy (EIS) has been proposed as the transduction principle for detecting the hybridization of DNA complementary strands. The resistance offered to the electrochemical reaction acts as the working signal, allowing an unlabelled gene assay. In our experiments, atypically large DNA oligonucleotides (110 base pairs) were used to determine the influence of ionic strength on the response of the DNA biosensor. In addition, the EIS response that was obtained by the hybridization process in the presence or absence of 6-mercapto-1-hexanol (MCH) was also evaluated. Finally, we extended these studies to probes and duplexes of various lengths observing a linear relationship between duplex length and the response (expressed as the R CT value).

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Section: Effect Of Ionic Strength Using 6-mercapto-1-hexanolmentioning

confidence: 86%

Effects of Ionic Strength and Probe DNA Length on the Electrochemical Impedance Spectroscopic Response of Biosensors

et al. 2010

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“…Upon hybridization, the rigidity of the double helix structure causes the probes to release from the substrate and stand upright. This change in the DNA orientation can create more ion paths for the redox compound to approach the electrode surface and consequently result in a reduction of the measured impedance (Gooding et al, 2003;Ito et al, 2007;Pan and Rothberg, 2005).…”

Section: Microfluidic Valved Loc For Dna Hybridization Analysismentioning

confidence: 99%

“…DNA hybridization detection is used extensively to diagnose genetic disorders (Chee et al, 1996;Ma et al, 2006) and various forms of cancer (Ito et al, 2007). For example, it is estimated that approximately 235,000 people will be diagnosed for breast cancer using DNA hybridization measurements in 2014 alone (Siegel et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

A controlled microfluidic electrochemical lab-on-a-chip for label-free diffusion-restricted DNA hybridization analysis

Ben‐Yoav

Dykstra

Bentley

et al. 2015

Biosensors and Bioelectronics

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“…Further investigation of these effects will result in guidelines for an accurate fabrication and operation of these devices in a reproducible manner that would improve upon their overall performance. DNA hybridization detection is used extensively to diagnose genetic disorders 24,25 and various forms of cancer 26 . Every year, multiple strains of influenza are identified in patients using results from DNA hybridization techniques 27 .…”

Section: Introductionmentioning

confidence: 99%

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis

Ben‐Yoav

Dykstra

Gordonov

et al. 2014

JoVE

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Miniaturization of analytical benchtop procedures into the micro-scale provides significant advantages in regards to reaction time, cost, and integration of pre-processing steps. Utilizing these devices towards the analysis of DNA hybridization events is important because it offers a technology for real time assessment of biomarkers at the point-of-care for various diseases. However, when the device footprint decreases the dominance of various physical phenomena increases. These phenomena influence the fabrication precision and operation reliability of the device. Therefore, there is a great need to accurately fabricate and operate these devices in a reproducible manner in order to improve the overall performance. Here, we describe the protocols and the methods used for the fabrication and the operation of a microfluidic-based electrochemical biochip for accurate analysis of DNA hybridization events. The biochip is composed of two parts: a microfluidic chip with three parallel micro-channels made of polydimethylsiloxane (PDMS), and a 3 x 3 arrayed electrochemical micro-chip. The DNA hybridization events are detected using electrochemical impedance spectroscopy (EIS) analysis. The EIS analysis enables monitoring variations of the properties of the electrochemical system that are dominant at these length scales. With the ability to monitor changes of both charge transfer and diffusional resistance with the biosensor, we demonstrate the selectivity to complementary ssDNA targets, a calculated detection limit of 3.8 nM, and a 13% cross-reactivity with other non-complementary ssDNA following 20 min of incubation. This methodology can improve the performance of miniaturized devices by elucidating on the behavior of diffusion at the micro-scale regime and by enabling the study of DNA hybridization events. Video LinkThe video component of this article can be found at

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Detection of single-base mismatch at distal end of DNA duplex by electrochemical impedance spectroscopy

Cited by 54 publications

References 17 publications

Effects of Ionic Strength and Probe DNA Length on the Electrochemical Impedance Spectroscopic Response of Biosensors

Effects of Ionic Strength and Probe DNA Length on the Electrochemical Impedance Spectroscopic Response of Biosensors

A controlled microfluidic electrochemical lab-on-a-chip for label-free diffusion-restricted DNA hybridization analysis

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis

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