Detection of Single Nucleotide Polymorphism by RNase H-Cleavage Mediated Allele-Specific Extension Method

Hou, Jingli; Liu, Xipeng; Liu, Jianhua

doi:10.5504/bbeq.2012.0048

Cited by 4 publications

(3 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Factors regulating R-loops ( Figure 2 A) include RNase H, which degrades the annealed RNA transcript in R-loops, thereby resolving these structures. RNA-DNA mismatches prevent RNAse H1-dependent R-loop resolution [ 17 ], and inactive RNAse H can be used to map R-loops [ 18 ]. In addition to specific degradation of the RNA strand, the helicase Senataxin (SETX) unwinds R-loops and enables subsequent cleavage [ 19 ].…”

Section: R-loops—physiological Appearance and Regulationmentioning

confidence: 99%

“…In addition to specific degradation of the RNA strand, the helicase Senataxin (SETX) unwinds R-loops and enables subsequent cleavage [ 19 ]. Other helicases, such as RNA helicase aquarius (AQR) and the DEAD Box helicase also resolve R-loops [ 1 , 5 , 9 , 17 , 20 , 21 ]. Similarly, topoisomerase I resolves supercoils, which prevents the formation of transcription-induced R-loops.…”

Section: R-loops—physiological Appearance and Regulationmentioning

confidence: 99%

See 1 more Smart Citation

R-Loops and R-Loop-Binding Proteins in Cancer Progression and Drug Resistance

Elsakrmy

Cui

2023

IJMS

View full text Add to dashboard Cite

R-loops are three-stranded DNA/RNA hybrids that form by the annealing of the mRNA transcript to its coding template while displacing the non-coding strand. While R-loop formation regulates physiological genomic and mitochondrial transcription and DNA damage response, imbalanced R-loop formation can be a threat to the genomic integrity of the cell. As such, R-loop formation is a double-edged sword in cancer progression, and perturbed R-loop homeostasis is observed across various malignancies. Here, we discuss the interplay between R-loops and tumor suppressors and oncogenes, with a focus on BRCA1/2 and ATR. R-loop imbalances contribute to cancer propagation and the development of chemotherapy drug resistance. We explore how R-loop formation can cause cancer cell death in response to chemotherapeutics and be used to circumvent drug resistance. As R-loop formation is tightly linked to mRNA transcription, their formation is unavoidable in cancer cells and can thus be explored in novel cancer therapeutics.

show abstract

Section: R-loops—physiological Appearance and Regulationmentioning

confidence: 99%

Section: R-loops—physiological Appearance and Regulationmentioning

confidence: 99%

R-Loops and R-Loop-Binding Proteins in Cancer Progression and Drug Resistance

Elsakrmy

Cui

2023

IJMS

View full text Add to dashboard Cite

show abstract

“…[16] Notably, purines (especially guanine, G) quench the fluorescence of most fluorophores by a photoinduced electron-transfer process; hence, turn-on fluorescence detection of purine repeats and mismatches has been quite a challenge. [17][18][19] Therefore, much of the recent effort in the development of new fluorescent nucleoside analogues is directed towards designing environment-sensitive analogues that have excitation and emission maximum in the visible region and high fluorescence efficiency within ONs, with the view of implementing them in both in vitro and in vivo assays. [5,[20][21][22][23] Kool and co-workers have assembled a library of DNA-like chains containing different PAH fluorophores ("oligodeoxyfluorosides") that display large Stokes shifts and a wide array of quantum yields and emission wavelengths.…”

Section: Introductionmentioning

confidence: 99%

Synthesis, Photophysical Properties and Incorporation of a Highly Emissive and Environment‐Sensitive Uridine Analogue Based on the Lucifer Chromophore

Tanpure

Srivatsan

2014

ChemBioChem

View full text Add to dashboard Cite

The majority of fluorescent nucleoside analogues used in nucleic acid studies have excitation maxima in the UV region and show very low fluorescence within oligonucleotides (ONs); hence, they cannot be utilised with certain fluorescence methods and for cell-based analysis. Here, we describe the synthesis, photophysical properties and incorporation of a highly emissive and environment-sensitive uridine analogue, derived by attaching a Lucifer chromophore (1,8-naphthalimide core) at the 5-position of uracil. The emissive nucleoside displays excitation and emission maxima in the visible region and exhibits high quantum yield. Importantly, when incorporated into ON duplexes it retains appreciable fluorescence efficiency and is sensitive to the neighbouring base environment. Notably, the nucleoside signals the presence of purine repeats in ON duplexes with an enhancement in fluorescence intensity, a property rarely displayed by other nucleoside analogues.

show abstract