Detection of somaclonal variation in micro-propagated Echinacea purpurea using AFLP marker

Chuang, Su Jean; Chen, Chung Li; Chen, Junne Jih; Chou, Wei Yu; Sung, Jih Min

doi:10.1016/j.scienta.2008.09.020

Cited by 38 publications

(24 citation statements)

References 31 publications

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“…The reasons accounting for somaclonal variations remain unclear, although factors such as culture duration, number of subcultures or transfers (Borse et al, 2011), phytoregulators (Biswas et al, 2009), explant type (Chuang et al, 2009), passage through the indirect callus phase (mass of undifferentiated cells with regeneration potential) (Peredo et al, 2006), genotype (Khan et al, 2009), culture medium composition (Lutts et al, 1998), and ploidy and mosaicism levels (Nakano et al, 2006) are considered capable of inducing this variability in vitro.…”

Section: Resultsmentioning

confidence: 99%

Genetic stability of micropropagated plants of Crambe abyssinica Hochst using ISSR markers

Werner¹,

Soares²,

Gontijo³

et al. 2015

Genet. Mol. Res.

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ABSTRACT. Crambe (Crambe abyssinica) is a non-edible annual herb, which was first cultivated to extract oil for industry, and now has great potential for biodiesel production. The objective of this investigation was to evaluate the genetic stability of micropropagated plants of the C. abyssinica Hochst cultivar 'FMS brilhante' using polymerase chain reaction techniques based on inter-simple sequence repeat (ISSR) molecular markers. The aim was to develop a protocol for the in vitro regeneration of these plants with low genetic variation as compared to the donor plant. For micropropagation, shoot tips from in vitro germinated seedlings were used as explants and were initially cultivated for 90 days on MS medium with 5.0 µM 6-benzylaminopurine (BAP), which at 90 days, led to the highest number of shoots per explant (NSE) (12.20 shoots) being detected. After 120 days, the interaction between BAP concentration and naphthalene acetic acid (NAA) was tested, and the highest NSE was observed following exposure to 0.0/0.5 µM BAP/NAA (11.40 shoots) and 1.0/0.0 µM BAP/NAA (11.00 shoots). The highest proportion of rooting phase were observed following exposure to 0.5 µM NAA (30%). The 13 ISSR primers used to analyze genetic stability produced 91 amplification products, of which only eight bands were polymorphic and 83 were monomorphic for all 10 regenerated crambe plants, compared to the donor plant explant. These results indicate that crambe shoot tips are a highly reliable explant that can be used to micropropagate genetically true-to-type plants or to maintain genetic stability, as verified using ISSR markers.

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Section: Resultsmentioning

confidence: 99%

Genetic stability of micropropagated plants of Crambe abyssinica Hochst using ISSR markers

Werner¹,

Soares²,

Gontijo³

et al. 2015

Genet. Mol. Res.

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“…Genomic DNA of each accession was extracted from freshly harvested young leaves by using CTAB (hexadecyltrimethylammonium bromide) procedure described by Chuang et al (2009). The samples of bulked leaf tissues (500 mg) collected from five individual plants were used for DNA extraction.…”

Section: Methodsmentioning

confidence: 99%

Genetic diversity in NaN3-induced common bean mutants and commercial varieties detected by AFLP

Jeng

et al. 2011

Crop Breed. Appl. Biotechnol.

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“…The explant tissue can affect the frequency and nature of somaclonal variation [38,39]. The use of meristematic tissues, such as the pericycle, procambium and cambium, as starting materials for tissue culture reduces the possibility of variation [40].…”

Section: Explant Sourcementioning

confidence: 99%

Somaclonal Variation in Tissue Culture: A Case Study with Olive

Leva¹,

Petruccelli²,

Rinaldi³

2012

Recent Advances in Plant in Vitro Culture

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Detection of somaclonal variation in micro-propagated Echinacea purpurea using AFLP marker

Cited by 38 publications

References 31 publications

Genetic stability of micropropagated plants of Crambe abyssinica Hochst using ISSR markers

Genetic stability of micropropagated plants of Crambe abyssinica Hochst using ISSR markers

Genetic diversity in NaN3-induced common bean mutants and commercial varieties detected by AFLP

Somaclonal Variation in Tissue Culture: A Case Study with Olive

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