Detection of some virulence factors in Pseudomonas aeruginosa associated with diarrhea in Kirkuk City

AL-Salihi, Siham Sh.; Hasan, Abbas Yaseen

doi:10.32894/kujss.2015.102016

Cited by 5 publications

(3 citation statements)

References 21 publications

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“…[4,25,26] The results of the current study agrees with [27], where they view that the isolates of the Citrobacter they ability product biofilm by the Congo red method. The ability of bacteria to produce extracellular enzymes as shown in table 3 that the isolates of S. aureus, P. aeruginosa and K. pneumonia were shown to produce urease and P. aeruginosa and E. cloacae were able to produce gelatin enzymes , The results also affirm that the isolates of S. aureus and P. aeruginosa were able to hemolysin on blood agar , The DNase test was performed on S. aureus isolates where it produces these enzymes extracellular enzyme is similar to most of the findings in the research [28][29][30].…”

Section: Resultssupporting

confidence: 78%

Isolation, Identification of Bacterial Species Causing Chronic suppurative Otitis Media and Detection Some of Their Virulence Factors

Mahmood¹,

Abed²,

Alwan³

2019

Tikrit J. Pure Sci.

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The study is conducted to diagnose the aerobic bacterial species causing chronic suppurative otitis media (CSOM), reveal the antibiotic susceptibility pattern and detect some of their virulence factors. Samples were collected during the period from June till December 2018. From a total of eighty-two patients admitted to Samarra Hospital and outpatient clinics of both genders with different age groups, 82 bacterial culture are recovered using a cotton swab. Identification of bacterial isolates is performed depending on micro and macroscopic cultural characteristics and biochemical tests. Results of the current work show that the highest infection rates are at the age groups >1 to 5 and 11 to 20 years by (20%). Among eight bacterial species isolated in the current study (S. aureus, P. aeruginosa, K.pneumonia, S.epidermidis, E.coli, P.vulgaris, C. freundii, E. Cloacae), S. aureus had scored the highest rate (41%) of the total infections while the lowest rate was scored by E.Cloacae(1%). The antibiotic sensitivity test suggests that almost all isolates were sensitive to ciprofloxacin and meropenem (96% and 94% respectively) while they were resistant to Cefixime. The ability of bacteria is isolated from CSOM to produce biofilm and some virulence factors (gelatinase, hemolysin, DNase, urease) are investigated the virulence factor results revealed that. S. aureus, P.aeruginosa, K. pneumonia had the ability to produce biofilm and S. aureus, P. aeruginosa have the ability the highest production for the majority of virulence factors.

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Section: Resultssupporting

confidence: 78%

Isolation, Identification of Bacterial Species Causing Chronic suppurative Otitis Media and Detection Some of Their Virulence Factors

Mahmood¹,

Abed²,

Alwan³

2019

Tikrit J. Pure Sci.

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“…Proteus mirabilis is a Gram-negative bacterium that belonging to the Enterobacteriaceae family and have the ability of changing in it is shape and using the flagella for swarming motility. It is causes several infections such as urinary tract infection, a middle ear infection, wounds and burn in addition to other infections [1,6]. It is an important opportunity bacterium that is found in water, soil and in the intestinal tracts of humans and animals.…”

Section: Introductionmentioning

confidence: 99%

Study the Effect of Biofilm Production on Antibiotic Resistance in Proteus mirabilis Isolated from Clinical Samples in Kirkuk City

Aziz Flamerz,

Sh. Obid,

M. Jasim

2023

NTU-JPS

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Proteus mirabilis is one of the most infectious organisms that inhabits the environment and causes a various infections involving those of the skin, respiratory tract, wounds and urinary tract. One of the main virulence features in P. mirabilis that plays a role in the pathogenesis and antibiotic resistance is the ability to form biofilm. The aim of study was to determine the relation between the antibiotic resistance and the biofilm formation by P. mirabilis. A total of (205) samples were collected from different clinical samples (urine, wound and burn swabs) in Kirkuk city hospitals. (32) Isolates were identified as P. mirabilis. Eight antibiotic discs were used for the antibiotic sensitivity test by disc diffusion method. Also, a micro titter plate method used for the detection of biofilm. The results of the antibiotic sensitivity showed variation in the rates of antibiotic resistance, doxycycline 29(90.6%), Trimethoprim- sulfamethaxole 27(84.37%), Ampicillin 22(68.75%) and amoxicillin/clavulanic 21(65.62%) resistance were found in the majority of the isolates. Gentamicin and cefotaxim (37.5%, 40.6%) respectively were moderate resistance, while Imipenem and Ciprofloxacin have low resistance and consider the most effective medication against P. mirabilis. The results showed 8 (25%) of P. mirabilis strong biofilm producer, 13 (41.6%) moderate biofilm producer, 8 (25%) weak biofilm producer and 3 (9.37%) non-biofilm producer. The study concluded that there is a link among biofilm production and antibiotic resistance.

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“…These infections become sever especially in immune compromised individuals (3). It also produces a number of virulence factors which after colonization can cause extensive tissue damage, bloodstream invasion, and dissemination (4).…”

Section: Introductionmentioning

confidence: 99%

Association of Multidrug Resistance With Biofilm Formation in Pseudomonas Aeruginosa Isolated from Clinical Samples in Kirkuk City

Rashad

Obaid

Al-kadhi

2022

NTU-JPS

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Pseudomonas aeruginosa is one of the most prevalent nosocomial pathogenic microorganisms that cause life-threatening infections. The formation of biofilm is one of the important features that lead resistance to antibiotics. The study aimed to clarify the relationship between biofilm formation, antibiotic resistance, and pigment production P. aeruginosa. A total of 33 isolates have been obtained from 220 different clinical samples, 42% of isolates obtained from burn swabs,15% wound swabs,12% from urine samples, 12% ear swabs ,6% sputum samples, and 3% from bronchial washes. The highest prevalence of these bacteria in our study was found in isolates obtained from burn swabs. Pyocyanin and pyoverdine are frequently seen on Cetrimide agar. P. aeruginosa can be completely isolated on Cetrimide agar. The production of biofilms detected by microtiter plate method and the result distributed as, 22% of strongly, 50% moderately, 27% weakly, and 4% were non biofilm producer. The Kirby-Bauer method (disc diffusion susceptibility method) was used to measure antibiotic resistance, and the results revealed a correlation as 86% multidrug resistance were strongly biofilm former, 75% multidrug resistance were moderately biofilm former and 11% multidrug resistance were weakly biofilm producers. Conclusion, P. aeruginosa from clinical isolates has highly proportional capability to form biofilm and recorded a positive correlation with multidrug resistance, while this relation was not significant with pigment production.

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Detection of some virulence factors in Pseudomonas aeruginosa associated with diarrhea in Kirkuk City

Cited by 5 publications

References 21 publications

Isolation, Identification of Bacterial Species Causing Chronic suppurative Otitis Media and Detection Some of Their Virulence Factors

Isolation, Identification of Bacterial Species Causing Chronic suppurative Otitis Media and Detection Some of Their Virulence Factors

Study the Effect of Biofilm Production on Antibiotic Resistance in Proteus mirabilis Isolated from Clinical Samples in Kirkuk City

Association of Multidrug Resistance With Biofilm Formation in Pseudomonas Aeruginosa Isolated from Clinical Samples in Kirkuk City

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