Detection of subgingival periodontal pathogens—comparison of two sampling strategies

Nickles, Katrin; Scharf, Susanne; Röllke, Lasse; Mayer, Irina; Mayer, Michael; Eickholz, Peter

doi:10.1007/s00784-015-1530-4

Cited by 13 publications

(26 citation statements)

References 39 publications

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“…This study protocol has been used previously to compare different sampling strategies in different cohorts of patients 7,24,25,27 …”

Section: Methodsmentioning

confidence: 99%

“…The following criteria led to exclusion from the study: 24 , 25 1) need for antibiotic prophylaxis before periodontal diagnosis or treatment; 2) antibiotic therapy within last 6 months or subgingival debridement (non‐surgical or surgical) within last 12 months before microbiologic sampling; and 3) application of commercial mouthwash on day of sample collection.…”

Section: Methodsmentioning

confidence: 99%

“…For this study, diagnoses of AgP was defined as: 7 , 24 , 25 1) clinically healthy patient (i.e., systemic diseases predisposing for periodontitis are not reported) (e.g., diabetes mellitus); 2) radiographic bone loss ≥50% at a minimum of two different teeth; and 3) aged ≤35 years. Diagnosis of CP was defined as: 1) vertical clinical attachment level (CAL‐V) ≥5 mm at >30% of sites; and 2) aged >35 years.…”

Section: Methodsmentioning

confidence: 99%

“…If CEJ was destroyed by restorative treatment, the margin of the restoration was taken as reference. Bleeding on probing was recorded 30 seconds after probing 24 , 25 …”

Section: Methodsmentioning

confidence: 99%

“…The main candidates have been patients showing further attachment loss even after adequate conventional therapy, patients with AgP or severe forms of periodontitis, or those with predisposing medical conditions 20 , 21 . In some studies detection of A. actinomycetemcomitans is one significant factor contributing to the decision whether mechanical anti‐infective therapy should be used in conjunction with systemic antibiotics 22–25 . As consumption of antimicrobials and spread of antimicrobial resistance in human and veterinary medicine is an increasing problem not only in Germany, 26 systemic antimicrobials should be prescribed in a more restrictive way.…”

mentioning

confidence: 99%

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Comparison of Two Different Sampling Methods for Subgingival Plaque: Subgingival Paper Points or Mouthrinse Sample?

Nickles

Scharf

Röllke

et al. 2017

Journal of Periodontology

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“…This study protocol has been used previously to compare different sampling strategies in different cohorts of patients 7,24,25,27 …”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

“…If CEJ was destroyed by restorative treatment, the margin of the restoration was taken as reference. Bleeding on probing was recorded 30 seconds after probing 24 , 25 …”

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

See 3 more Smart Citations

Comparison of Two Different Sampling Methods for Subgingival Plaque: Subgingival Paper Points or Mouthrinse Sample?

Nickles

Scharf

Röllke

et al. 2017

Journal of Periodontology

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Microbial signatures of oral dysbiosis, periodontitis and edentulism revealed by Gene Meter methodology

Hunter

Pozhitkov

Noble

2016

Journal of Microbiological Methods

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Conceptual models suggest that certain microorganisms (e.g., the "red" complex) are indicative of a specific disease state (e.g., periodontitis); however, recent studies have questioned the validity of these models. Here, the abundances of 500+ microbial species were determined in 16 patients with clinical signs of one of the following oral conditions: periodontitis, established caries, edentulism, and oral health. Our goal was to determine if the abundances of certain microorganisms reflect dysbiosis or a specific clinical condition that could be used as a 'signature' for dental research. Microbial abundances were determined by the analysis of 138,718 calibrated probes using Gene Meter methodology. Each 16S rRNA gene was targeted by an average of 194 unique probes (n=25nt). The calibration involved diluting pooled gene target samples, hybridizing each dilution to a DNA microarray, and fitting the probe intensities to adsorption models. The fit of the model to the experimental data was used to assess individual and aggregate probe behavior; good fits (R>0.90) were retained for back-calculating microbial abundances from patient samples. The abundance of a gene was determined from the median of all calibrated individual probes or from the calibrated abundance of all aggregated probes. With the exception of genes with low abundances (<2 arbitrary units), the abundances determined by the different calibrations were highly correlated (r~1.0). Seventeen genera were classified as 'signatures of dysbiosis' because they had significantly higher abundances in patients with periodontitis and edentulism when contrasted with health. Similarly, 13 genera were classified as 'signatures of periodontitis', and 14 genera were classified as 'signatures of edentulism'. The signatures could be used, individually or in combination, to assess the clinical status of a patient (e.g., evaluating treatments such as antibiotic therapies). Comparisons of the same patient samples revealed high false negatives (45%) for next-generation-sequencing results and low false positives (7%) for Gene Meter results.

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A Study on the Use of Phase Transition Lysozyme-Loaded Minocycline Hydrochloride in the Local Treatment of Chronic Periodontitis

Wang

Deng

Zhang

et al. 2022

ACS Appl. Bio Mater.

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Periodontitis is the most important oral disease causing human tooth loss. Although supragingival and subgingival scaling is the main strategy of periodontitis clinical treatments, drug treatment has an indispensable auxiliary role to some degree. Periodontitis medical treatment is divided into systemically administered treatments and local periodontally administered treatments. Compared with systemic administration, local administration can increase local drug concentrations, reduce dosages, and prolong action times while also improving patient compliance and avoiding possible adverse effects due to systemic administration responses. However, some studies show that minocycline ointment, a clinical local drug commonly used in periodontal pockets, has an unstable release rate; 80% of the drug is usually released within 2–3 days after pocket placement. This release is not conducive to controlling periodontal infection and may hinder the periodontal tissue repair and regeneration. Therefore, choosing a suitable carrier for minocycline hydrochloride is necessary to control its local release in periodontal tissue. Phase transition lysozyme (PTL) has been widely used in many studies and the development of macromolecular carrier material, and we selected PTL as the carrier for minocycline hydrochloride drugs because of its good biocompatibility, good drug-carrying capacity, and stable release. Due to its release characteristics and simple preparation, PTL is a promising carrier material.

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Detection of subgingival periodontal pathogens—comparison of two sampling strategies

Abstract: In daily dental practice, the plaque sampling of the deepest pockets per quadrant seems to be sufficient.

Cited by 13 publications

References 39 publications

Comparison of Two Different Sampling Methods for Subgingival Plaque: Subgingival Paper Points or Mouthrinse Sample?

Comparison of Two Different Sampling Methods for Subgingival Plaque: Subgingival Paper Points or Mouthrinse Sample?

Microbial signatures of oral dysbiosis, periodontitis and edentulism revealed by Gene Meter methodology

A Study on the Use of Phase Transition Lysozyme-Loaded Minocycline Hydrochloride in the Local Treatment of Chronic Periodontitis

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