Detection of Sulfamethazine Residues in Milk by Surface Plasmon Resonance-Based Biosensor Inhibition Immunoassay

Abstract

A method for detection of sulfamethazine（SMT）in milk by coupling indirect inhibition assay with a self-assembled monolayer was developed. The method of immobilization of SMT-BSA on the chip surface was also introuduced. A series of milk samples with spiked 0, 5, 10, 20, 30, 40 ng/mL of SMT were analyzed by the SPR biosensor and the standard calibration curve of SMT in milk was constructed. The intra-assay relative standard deviation (RSD) and recovery were 6.3% and 102.8%, respectively. The inter-assay deviation (7 days) was less than 6 IU, and demonstrated the method’s stability. The high stability of the SPR sensor underlies the potential of the SPR method as a stable and high-precision tool for detection of SMT.

Interfacial Influence of Strontium Niobium Engulfed Reduced Graphene Oxide Composite for Sulfamethazine Detection: Employing an Electrochemical Route in Real Samples

“…9,10 Hence, concerning the harmful impacts, the European Commission and several countries like the USA, China, Canada have given the maximal degree of 100 μg kg −1 as their inhibition level. 11 SMZ bears a p-amino phenyl sulfonamide structure and could cause an imbalance in microscopic organisms surviving in the human body, which primes various infections. 12,13 Growing requisite for SMZ recognition has induced diverse systems as polar integrative sample technique, immunoassays, surface plasma resonance, capillary electrophoresis, biosensors, and so on.…”