A method for detection of sulfamethazine（SMT）in milk by coupling indirect inhibition assay with a self-assembled monolayer was developed. The method of immobilization of SMT-BSA on the chip surface was also introuduced. A series of milk samples with spiked 0, 5, 10, 20, 30, 40 ng/mL of SMT were analyzed by the SPR biosensor and the standard calibration curve of SMT in milk was constructed. The intra-assay relative standard deviation (RSD) and recovery were 6.3% and 102.8%, respectively. The inter-assay deviation (7 days) was less than 6 IU, and demonstrated the method’s stability. The high stability of the SPR sensor underlies the potential of the SPR method as a stable and high-precision tool for detection of SMT.

To gain new insights into the underlying mechanisms responsible for farmland microclimate and yield of winter wheat under different row spacings [row spacing: 7 cm (RS7); 14 cm (RS14); 24.5 cm (RS24.5); 49 cm (RS49)], field experiments were conducted at Taian, China. Between the hours 13:00 and 14:00, low values of relative humidity (RH) and air temperature at 5 cm above the ground for the various row spacings were obtained. The average RH of RS7, RS14, RS24.5, and RS49 was 91.0%, 90.3%, 85.7%, and 76.4%, respectively; the average air temperature were 22.6°C, 23.2°C, 24.3°C and 25.3°C, respectively. The soil temperature of RS49 was higher than those of RS7 and RS14. The maximum values of eddy thermal diffusivity was 0.07 m 2 s -1 , as well as sensible and soil heat fluxes, were obtained at 12:00 o'clock. The order of these parameters was similar to that of the air temperature, but opposite to that of the latent heat flux. The yield of RS49 was significantly lower than those of the other treatments (P < 0.05). The present study indicates that excessive row spacing may result in severe dissipation of energy. RS14 is the optimum condition under uniform planting density. 2 akışının yanı sıra en yüksek termal difüzyon katsayısı 0.07 m 2 s -1 olup bu değer saat 12'de ölçülmüştür. Bu parametrelerin sıralaması hava sıcaklığı parametrelerinin sıralamasına benzer olmuş ancak gizli ısı akışı parametrelerinin sıralamasının tam tersi bir durum göstermiştir. Sıra arası 49 cm olduğu durumdaki uygulamanın diğerlerinden belirgin bir şekilde daha düşük olmuştur (P < 0.05). Araştırma sonuçlarına göre, sıra arası mesafesinin artması, enerji kaybında büyük kayıplara yol açtığını göstermiştir. Düzgün bir bitki dağılımı için en uygun koşullar açısndan sıra arası mesafenin 14 cm olduğu belirlenmiştir.

To obtain optimal medical ceramic film with bioactivity, Microarc oxidized TiO2-based films containing Ca and P on titanium alloy were formed in electrolytes containing hydroxyapatite and β-tricalcium phosphate salts. After that the biocompatibility tests including invitro soaking test and invivo implant experiment were carried out, and component and phases of films were analysed by energy dispersive spectrometer and X-ray diffraction, respectively.The obtained result shows the porosity biomedical ceramic films with different proportional of Hydroxyapatite/β-tricalcium are fabricated through changing the electrolyte composions,respectively.The bioactivity of biphasic calcium phosphate is enhanced comparing to the pure HA ones.So the biphasic calcium phosphate film material can be satified with medical application.

The fatigue limit of parts and components that have the multi-notches is important data for the design and manufacture of machinery and traffic equipment which are operated under the high speed or pressure. In this paper the rotating bending fatigue tests have been carried out to investigate the fatigue limit of specimen with double-notch that is constructed of step and blind hole, and analyzed the effect of stress concentrations at the double-notched bottoms on the fatigue limits, using three-dimensional elastic finite element method. Firstly, the fatigue tests of 8 group specimens have been performed for examining the of fatigue limits of the single-notched specimen and double-notched specimen, respectively. Additionally, the stress field interactions between two stress fields by the blind hole notch and step are discussed using three-dimensional elastic finite element method. The main results obtained in this study are as follows: The fatigue limit of the double-notched specimen are down comparison with the fatigue limit of the single-notched specimen; the fatigue limit of the double-notch specimen is insensitive to distance between the blind hole and step for the low carbon structure steel with better ductility; for the high-strength steel, superposition and intensification of the stress concentration by the blind hole and step mutually may be avoided so that their adverse effects on the fatigue strength may be become to minimize, as take appropriate distance between the blind hole and step. The results are significant for the design of engineering design of the multi-notched parts, and the study of fatigue strength.