Detection of supported lipid layers with the acoustic Love waveguide device: application to biosensors

In this work we present a novel pulse mode Love wave biosensor that monitors both changes in amplitude and phase. A series of concentrations of 3350 molecular weight poly(ethylene glycol) (PEG) solutions are used as a calibration sequence for the pulse mode system using a network analyzer and high frequency oscilloscope. The operation of the pulse mode system is then compared to the continuous wave network analyzer by showing a sequence of deposition and removal of a model mass layer of palmitoyl-oleoyl-sn-glycerophosphocholine (POPC) vesicles.This experimental apparatus has the potential for making many hundreds of measurements a minute and so allowing the dynamics of fast interactions to be observed.

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“…The greater attenuation obtained during the first POPC deposition is consistent with previously published work [9][10] .…”

Section: Resultssupporting

confidence: 92%

Pulse mode operation of Love wave devices for biosensing applications

Newton

McHale

Martin

et al. 2001

Analyst

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“…The rise to the initial insertion loss after the detergent treatment suggests that the POPC is fully removed by the Triton. The greater attenuation obtained during the first POPC deposition is consistent with previously published data [9].…”

supporting

confidence: 92%

“…The biological mass layer within the penetration depth thus changes and an 2 acoustic signal response is observed [9]. Here we present data showing a Love wave device operated in a frequency hopping mode utilizing both the fundamental and third harmonic during a biosensing experiment.…”

mentioning

confidence: 84%

Harmonic Love wave devices for biosensing applications

et al. 2001

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“…Different guiding layer materials have been reported such as polymethylmethacrylate (PMMA) [9,10] positive photoresists [11] SiO 2 [12] and multi-layer polymer/SiO 2 structures [13]. Although silica guiding layers display low acoustic losses in the MHz range their fabrication by deposition techniques is lengthy, and thick layers (~6 μm) are required to support a Love wave if quartz substrates are used due to the materials' similar acoustic velocities [14].…”

Section: Introductionmentioning

confidence: 99%

SU-8 Guiding Layer for Love Wave Devices

Roach

Atherton

Doy

et al. 2007

Sensors

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SU-8 is a technologically important photoresist used extensively for the fabrication of microfluidics and MEMS, allowing high aspect ratio structures to be produced. In this work we report the use of SU-8 as a Love wave sensor guiding layer which allows the possibility of integrating a guiding layer with flow cell during fabrication. Devices were fabricated on ST-cut quartz substrates with a single-single finger design such that a surface skimming bulk wave (SSBW) at 97.4 MHz was excited. SU-8 polymer layers were successively built up by spin coating and spectra recorded at each stage; showing a frequency decrease with increasing guiding layer thickness. The insertion loss and frequency dependence as a function of guiding layer thickness was investigated over the first Love wave mode. Mass loading sensitivity of the resultant Love wave devices was investigated by deposition of multiple gold layers. Liquid sensing using these devices was also demonstrated; water-glycerol mixtures were used to demonstrate sensing of densityviscosity and the physical adsorption and removal of protein was also assessed using albumin and fibrinogen as model proteins.

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Detection of supported lipid layers with the acoustic Love waveguide device: application to biosensors

Cited by 47 publications

References 9 publications

Pulse mode operation of Love wave devices for biosensing applications

Pulse mode operation of Love wave devices for biosensing applications

Harmonic Love wave devices for biosensing applications

SU-8 Guiding Layer for Love Wave Devices

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