Detection of Tast Salt Tolerance Gene in Three Selected Wheat Genotypes for Salinity Stress Under Salinity Conditions

Abstract

The aim of this study is detection of salt tolerant gene (TaST) selected wheat genotypes for salt tolerance (1H, 2H , 3H) through plant breeding program as compared with local cultivars (sensitive) under salinity condition. Quantitative reverse transcription-PCR (q-RT-PCR) was used to detect TaST salt tolerant gene in these selected genotypes. Also chlorophyll and organic compounds contents were estimated in the upper leaves of the selected genotypes and local cultivars. Results of the PCR showed that the gene band of TaST appeared only in the selected genotypes with length 175bp at both salinity conditions, while this band absent in the local cultivars, (sensitive) (Iraq and latefyi), also at both salinity conditions. All the selected genotypes had the same band size (175bp). Chlorophyll content in the upper leaves of selected genotypes increased at salinity treatment, while it decreased in the leaves of local cultivars at this treatment as compared with non-saline conditions. The results also, showed that the selected genotypes had the highest organic compounds in their leaves, especially under salinity condition. It can be concluding that the selected genotypes (1H, 2H, and 3H) were more salt tolerance than local cultivars (Iraq, latefyi).

Evaluation of the Biological Phenomenon of Cadmium Accumulation of Wheat Crop in Salt-Affected Soil

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Carbon-Sequestering Fertilizers Usage to Boost Potassium Efficiency in Wheat Growth Under Saline Conditions

“…Furthermore, a decline in the translocation of assimilated products occurs under salinity stress. An inhibited germination and growth, hormonal imbalance, oxidative stress, and yield reductions are the possible outcomes of plant exposure to saline growth media (Majeed, 2018;Saddiq et al, 2021;Seleiman et al, 2022). Plants need balanced nutrition; therefore, they uptake nutrients from the soil essential for growth; one of these is K, one of the 19 crucial elements.…”

Exploring the tolerance of Iraqi wheat varieties: Evaluating seed germination and early growth of six Iraqi wheat varieties under salinity stress

“…Mishandled irrigation, high evaporation rate and inadequate precipitation increased the size of saline areas in arid and semiarid regions (Dai et al, 2011;Jamil et al, 2011). Environmental conditions lead to water stress in plants, for instance, saline habitats have high salt concentrations that lead to difficulties for roots to absorb water from the soil lead to a reduction in some morphological parameters such as vegetative growth and leaf area and physiological parameters such as photosynthesis and stomatal conductance reduction (Majeed, 2018;Saddiq et al, 2021). Wheat Triticum aestivum is classified as the most important crop globally besides maize and rice which is significantly considered as a part of daily calories and protein and it ranked first for its values in domestication and staple food (Al-Hachami & Frhan, 2018;Iqbal et al, 2021).…”