Detection of tetanus toxoid antibodies in human sera in New Zealand by ELISA

Abstract

SUMMARYAn enzyme-linked immunosorbent assay (ELISA) incorporating the sensitive biotin-streptavidin system was developed to detect IgG antibodies to tetanus toxoid in human serum. Serum samples obtained from 557 normal persons aged 1-65 years from different areas in New Zealand were tested. The proportion of those immune ranged from 60-93 % in males, and from 46-86 % in females. In the 1-9 years age group 85 % were immune. The indirect ELISA is suitable for serological surveys as it is simple to perform, economical and reproducible.

Tetanus incidence and immunity in Poland

“…A higher immunity status is seen in many countries where men have a better chance to be immunized than women (Fig. 5) (1, 7,8,10,21,24,29). In Denmark, military recruits are immunized or re-immunized with the child-type DT vaccine containing 12.5 Lf of diphtheria and 12 Lf of tetanus toxoid (22,23).…”

“…The overall reported incidence of tetanus has declined gradually since 1960; in the last three years less than 100 cases were reported (Table 2). Poland 1960, 1965, 1975-1987Age groups 1960196519751987 (years) Although the incidence rate continues to be higher in rural than in urban areas, an increasing number of provinces are recording zero cases; over the last 4 years the proportion of tetanus-free provinces has increased from 22% to 39%.…”

The Present Status of Tetanus and Tetanus Vaccination

“…Studies show an increase in immunity among Indian women (MISRA and RAO 1988) and Burmese Poland 1960-1987. (From GALAZKA and KARDYMOWICZ 1989 male military staff (MYA et al 1985).…”