Detection of the Effective DNA Protection by Quinazolines Using a DNA‐Based Electrochemical Biosensor

Ovádeková, Renáta; Jantová, Soňa; Labuda, Ján

doi:10.1080/00032710500371071

Cited by 10 publications

(6 citation statements)

References 27 publications

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“…Both procedures used showed a rapid decrease of surviving DNA with an increase of the AO concentration. A correlation of the results with our previous AO investigation 32 can be considered as a validation of the CV and EIS procedures reported in this paper.…”

Section: Detection Of Deep Dna Damagementioning

confidence: 97%

Disposable Electrochemical Biosensor with Multiwalled Carbon Nanotubes-Chitosan Composite Layer for the Detection of Deep DNA Damage

2008

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A novel electrochemical DNA-based biosensor for the detection of deep DNA damage was designed employing the bionanocomposite layer of multiwalled carbon nanotubes (MWNT) in chitosan (CHIT) deposited on a screen printed carbon electrode (SPCE). The biocomponent represented by double-stranded (ds) herring sperm DNA was immobilized on this composite using layer-by-layer coverage to form a robust film. Individual and complex electrode modifiers are characterized by a differential pulse voltammetry (DPV) with the DNA redox marker [Co(phen)3] 3+ , cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) with [Fe(CN)6] 3-as a redox probe in a phosphate buffer solution (PBS). A good correlation between the CV and EIS parameters has been found, thus confirming a strong effect of MWNT on the enhancement of the electroconductivity of the electrode surface and that of CHIT on the MWNT distribution at the electrode surface. Differences between the CV and EIS signals of the electrodes without and with DNA are used to detect deep damage to DNA, advantageously using simple working procedures in the same experiment.

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Section: Detection Of Deep Dna Damagementioning

confidence: 97%

Disposable Electrochemical Biosensor with Multiwalled Carbon Nanotubes-Chitosan Composite Layer for the Detection of Deep DNA Damage

2008

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“…Labuda and co-workers have developed a redox indicator-based method for detection of DNA double helix disruption using intercalators binding specifically to immobilized dsDNA and producing a redox signal at carbon electrodes. Upon DNA degradation, the indicator signal decreased due to its lower affinity toward ssDNA. − The method was applied for studying DNA damage induced by nitrofluorenes, tin(II) or arsenic(III) compounds, or hydroxyl radicals, or for evaluation of antioxidative properties of various flavonoids …”

Section: Detection Of Dna Damagementioning

confidence: 99%

Electrochemistry of Nucleic Acids

2012

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“…This accelerates the DNA oxidation in the presence of dissolved oxygen as a source for catalytic formation of superoxide anion radical. Such reactions were described for bipyridyl 20–22 and phenanthroline 23–25 complexes of Ru, Co and some other transient metals. Changes in the DNA structure caused by its damage influence the intercalation of redox active species, e.g., Methylene blue that changes their redox activity on the electrode 26–28.…”

Section: Introductionmentioning

confidence: 99%

Impedimetric Detection of DNA Damage with the Sensor Based on Silver Nanoparticles and Neutral Red

et al. 2015

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1IntroductionSince 1990, deoxyribonucleica cid (DNA) has been utilized as biorecognition element in the assembly of electrochemical biosensors.T he interest to DNAa satarget and tool of biochemical assayi sr elated to critical importance of this biomolecule in the storage and transfero f genetici nformation [1,2].T he investigation of molecular recognition with participation of DNAi sd emanded in molecular biology,t oxicology,m edicine,p harmacy and many other areas of research activity considering human health problems.I na ddition to detection of DNAÀana-lyte interactionsi ncluding genetic polymorphism,h ybridization events etc.,t he DNAd amage,w hich potentially results in many diseases like cancer, is recentlyi nt he focus of many research efforts [3,4].D ifferentt ypes of the DNAd amage induced by reactiveo xygen species [5], UV exposure[ 6],a nd mutagenic species [7][8][9] were successfully detectedbyb iosensor approaches.Fast screening of DNAd amage is vital for understanding the mechanisms and establishing necessary preventive measures.T he electrophoresis mainly applied for detection of the DNAs trand breaks [10] is rather sensitiveb ut cannot be used in field conditions. Electrochemical DNAbased biosensors are portable devices integrating DNAa s biorecognitionelementand an electrode as physicochemical transducer [ 11].T he progress in detection of the DNAd amage by biosensors is caused by undisputed advantages that involves imple design and measurement protocol, sufficient sensitivity,c ompatibility with conventional electrochemical equipment and analogy to real redox-processes that take place in living cells under oxidative stress.I ts hould be also mentioned that many of the protocols of the DNAd amage detection are applied for the estimationo fp rotecting effecte xerted by antioxidants.A ssessment of the antioxidant activity is required in agriculture and food quality analysis [6,[12][13][14].Electrochemical detection of the oxidativeD NA damage is mainly based on recording signals referred to selected nucleic basesa nd productso ft heir transformation [15][16][17][18]. Thus,t he accessibility of guaninef or direct oxidation on the electrode increased withD NA helix distortion. Fort he samer eason, increased peak of guanine oxidation is often followed by appearance of the peak of biomarker of its oxidation, 8-oxo-guanine [19].G uanine oxidation can be promoted by redox mediators either directly or via formation of oxygen radical species.T his acceleratest he DNAo xidationi nt he presence of dissolved oxygen as as ource for catalytic formationo fs uperoxide anionr adical. Such reactionsw ere describedf or bipyridyl [20][21][22] and phenanthroline [23][24][25] complexes of Ru, Co and some other transient metals.C hanges in the DNA structurec ausedb yi ts damage influence the intercalation of redox active species,e .g.,M ethyleneb lue thatc hanges Abstract:N ovele lectrochemical DNA-sensor based on glassy carbon electrode( GCE) modified with Ag nanoparticles,N eutral red covalently attached to its surfa...

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Detection of the Effective DNA Protection by Quinazolines Using a DNA‐Based Electrochemical Biosensor

Cited by 10 publications

References 27 publications

Disposable Electrochemical Biosensor with Multiwalled Carbon Nanotubes-Chitosan Composite Layer for the Detection of Deep DNA Damage

Disposable Electrochemical Biosensor with Multiwalled Carbon Nanotubes-Chitosan Composite Layer for the Detection of Deep DNA Damage

Electrochemistry of Nucleic Acids

Impedimetric Detection of DNA Damage with the Sensor Based on Silver Nanoparticles and Neutral Red

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