Detection of the enterotoxigenic genes (sei,sej) in Staphylococcus aureus isolates from bovine mastitis milk in the West Azerbaijan of Iran

Ahmady, Malahat; Kazemi, Sima

doi:10.1007/s00580-012-1460-3

Cited by 14 publications

(10 citation statements)

References 49 publications

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“…Bovine mastitis is a major disease affecting the dairy industry worldwide with huge economic loss and decreased animal health (Ahmady and Kazemi, 2013;Deb et al, 2013;Gomes and Henriques, 2016). Although mastitis is often caused by a wide variety of pathogens, such as bacteria, viruses, fungi, mycoplasma, and others, Staphylococcus aureus is still described as one of the most frequently isolated etiological agents associated with bovine intramammary infections (Momtaz et al, 2010;Lundberg et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial susceptibility, virulence genes, and randomly amplified polymorphic DNA analysis of Staphylococcus aureus recovered from bovine mastitis in Ningxia, China

Dong

Zhang

Zhou

et al. 2016

Journal of Dairy Science

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Staphylococcus aureusis the leading pathogen involved inbovine mastitis, but knowledgeabout antimicrobial resistance, virulence factors, and genotypes of Staphylococcus aureus resulting in bovine mastitis in Ningxia, China, is limited. Therefore, antimicrobial susceptibility, virulence gene, and randomly amplified polymorphic DNA (RAPD) analyses of Staph. aureus were carried out. A total of 327 milk samples from cows with clinical and subclinical mastitis in 4 regions of Ningxia were used for the isolation and identification of pathogens according to phenotypic and molecular characteristics. Antimicrobial susceptibility against 22 antimicrobial agents was determined by disk diffusion. The presence of 8 virulence genes in Staph. aureus isolates was tested by PCR. Genotypes of isolates were investigated based on RAPD. Results showed that 35 isolates obtained from mastitis milk samples were identified as Staph. aureus. The isolates were resistant to sulfamethoxazole (100%), penicillin G (94.3%), ampicillin (94.3%), erythromycin (68.6%), azithromycin (68.6%), clindamycin (25.7%), amoxicillin (11.4%), and tetracycline (5.7%). All of the isolates contained one or more virulence genes with average (standard deviation) of 6.6±1.6. The most prevalent virulence genes were hlb (97.1%), followed by fnbpA, hla, coa (94.3% each), nuc (85.7%), fnbpB (80%), clfA (77.1%), and tsst-1 (40%). Nine different gene patterns were found and 3 of them were the dominant gene combinations (77.1%). Staphylococcus aureus isolates (n=35) were divided into 6 genotypes by RAPD tying, the genotypes III and VI were the most prevalent genotypes. There was greatvariation in genotypes of Staph. aureus isolates, not only among different farms, but also within the same herd in Ningxia province. The study showed a high incidence of Staph. aureus with genomic variation of resistance genes, which is matter of great concern in public and animal health in Ningxia province of China.

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Section: Introductionmentioning

confidence: 99%

Antimicrobial susceptibility, virulence genes, and randomly amplified polymorphic DNA analysis of Staphylococcus aureus recovered from bovine mastitis in Ningxia, China

Dong

Zhang

Zhou

et al. 2016

Journal of Dairy Science

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“…It causes a fall in milk production, decreased milk quality, economic diagnostic and it's considered of quite vital importance to the public health due to its association with many zoonotic diseases in which the milk act as a vehicle for some infectious agents [3]. The most common mastitis pathogens are caused by a wide variety of bacteria, which can be classified as environmental (Escherichia coli, Streptococcus dysgalactiae, Streptococcus uberis, Enterococcus spp.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of antibacterial effect of some Sinai medicinal plant extracts on bacteria isolated from bovine mastitis

Zeedan¹,

Abdalhamed²,

Abdeen³

et al. 2014

Vet World

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Evaluation of antibacterial effect of some Sinai medicinal plant extracts on bacteria isolated from bovine mastitis, Veterinary World, 7(11): 991-998. AbstractAim: Bovine mastitis is the most economically important disease affecting dairy cattle worldwide from an economic, diagnostic and public-health point of view. The present study aimed to isolate and identify of bacteria causes mastitis in dairy cows and to evaluate the antibacterial activities of some selected medicinal plants extracts comparing antibiotics used in the treatment of mastitis in Egypt. Materials and Methods:A total of 203 milk samples of dairy cows were collected during the period from February to June 2013 at different Governorates in Egypt. The use clinical inspection and California mastitis test examination were provided efficient diagnostic tool for detection of clinical, subclinical mastitis and apparently normal health cattle. The collected milk samples were cultured on Nutrient, Blood agar, Mannitol salt, Edward's and MacConkey agar plates supporting the growth of various types of bacteria for their biochemical studies and isolation. The antimicrobial activity of plants extracts (Jasonia montana and Artemisia herb alba)with different solvent (ethanol, petroleum ether, chloroform and acetone)were studied in vitro against isolated bacteria from mastitis by paper desk diffusion and minimum inhibitory concentration method (MIC). Results:The prevalence of clinical, subclinical mastitis and normal healthy animals were 34.50%, 24.7% and 40.8% respectively. The major pathogens isolated from collected milk samples were Escherichia coli The MIC values for the extracts ranged from 0.01 to 1.56 mg/ml. when comparing antibacterial activity of A. herb alba plant extracted with acetone solvent on the same bacteria with zone of inhibition values ± SD, ranging from 00±00 to 5.6±0.60 mm. Both extracts from J. montana and A. herb alba plant extracts with petroleum ether, methanol and chloroform solvent were less antibacterial activities than acetone solvent extract. Conclusion:The present study spot highlight on isolation and identification of mastitis pathogens that are fundamental aspects of milk quality, udder health control programs and public health and food safety issues associated with food borne pathogens. J. montana and A. herb alba plants have antibacterial effects more than antibiotics used in the treatment of mastitis. Finally, the medicinal plant extracts can be used to discover bioactive natural product in the form of antibacterial that may be serve the development of new pharmaceutical products. But still need further research necessary to identify active compounds and research to mechanism and drug interaction.

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“…It is considered as one of the economically important diseases of livestock, since it causes a fall in milk production, decreased milk quality for dairy purposes and results in additional costs for veterinary services and laboratory investigations (Halasa et al, 2007;Ahmady and Kazemi, 2013). In addition to the risk factors such as milking hygiene, management practice, stage of lactation and parity, several prokaryotic and eukaryotic pathogens (especially bacterial agents) have been identified to be associated with the onset of mastitis in animals (Boscos et al, 1996;Bhatt et al, 2011;Keane et al, 2013;Raemy et al, 2013).…”

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confidence: 99%

Isolation and Identification of Bacterial Agents Causing Clinical Mastitis in Cattle in Mymensingh and Their Antibiogram Profile

2014

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A B S T R A C TMastitis is a serious problem for the dairy cattle in many countries of the world including Bangladesh. Among the microbial etiology bacteria plays a major role in the onset of the clinical form of the disease. Many of these bacteria are resistant to one or more antibiotics thus make the mastitis cases difficult to cure. In the present study Staphylococcus spp., Streptococcus spp., Bacillus spp. and E. coli were identified as the dominant bacterial species causing clinical mastitis in cattle in Mymensingh. Antibiogram study revealed chloramphenicol and erythromycin as the most effective antibiotic for the treatment of mastitis in these animals except those caused by Bacillus spp. and E. coli, respectively.

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Detection of the enterotoxigenic genes (sei,sej) in Staphylococcus aureus isolates from bovine mastitis milk in the West Azerbaijan of Iran

Cited by 14 publications

References 49 publications

Antimicrobial susceptibility, virulence genes, and randomly amplified polymorphic DNA analysis of Staphylococcus aureus recovered from bovine mastitis in Ningxia, China

Antimicrobial susceptibility, virulence genes, and randomly amplified polymorphic DNA analysis of Staphylococcus aureus recovered from bovine mastitis in Ningxia, China

Evaluation of antibacterial effect of some Sinai medicinal plant extracts on bacteria isolated from bovine mastitis

Isolation and Identification of Bacterial Agents Causing Clinical Mastitis in Cattle in Mymensingh and Their Antibiogram Profile

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