Detection of the extended spectrum β-lactamase produced by Escherichia coli from dairy cows by using the Vitek-2 method in Tulungagung regency, Indonesia

Abstract

The use of antibiotics in inappropriate on food producing animals can lead to resistance many of the pathogenic bacteria to the various types of antibiotics, one of which is the Escherichia coli (E. coli) which produces extended spectrum β-lactamase (ESBL). Antibiotic resistance in animals and humans has become a global problem that needs attention and immediate management by using specific antibiotics that used for therapeutic the infected animals. The aim of this study was to isolate and detect E. coli producing ESBL. All E. coli from the surface of dairy cow rectal swabs in Sendang District, Tulungagung Regency, Indonesia using the Vitek-2 method. The number of rectal swab samples used in the present study was 50. The results of this study showed that all the samples were suspected of being E. coli, based on the morphological growth of colonies on the EMBA media. The isolates were identified by using the biochemical tests. All the samples were positive. In this study the double disc synergy test (DDST) method was using to confirm the ESBL. The antibiotics were used amoxicylyn-clavulanate, ceftazidime and cefotaxime for DDST. In additional ESBL confirmation test was used the Vitek-2 method. The presence of ESBL producing by E. coli isolated from rectal dairy swabs in tulungagung was 6% (3/50).

Opportunistic Bacteria Isolated from Trypauchen Vagina Fish that Infected with Protozoan from Iraq Marine Water

“…Some bacteria appears to be resistant to antibiotics, even with maximum doses, according to Janda and Abbot (2010). The resistance of microorganisms to antibiotics develops as resistance to them occurs (Putra et al, 2019). The results showed that A. hydrophila is resistant to gentamicin, streptomycin, tetracycline and amoxicn, which is similar to the results observed by Hazen et al (1978).…”

Presence of multidrug resistance (MDR) and extended-spectrum beta-lactamase (ESBL) of Escherichia coli isolated from cloacal swab of broilers in several wet markets in Surabaya, Indonesia

“…Samples were cultured on Mac Conkey Agar (MCA) media for 24 hours at 37ºC. The bacterial colonies on MCA media were observed for color and texture and processed for identification of E. coli Putra et al 2020).…”

Short Communication: Pattern of antibiotic resistance on extended-spectrum beta-lactamases genes producing Escherichia coli on laying hens in Blitar, Indonesia

“…The antibiotic discs of amoxicillin-clavulanic 30μg (Oxoid, England), cefotaxime 30μg (Oxoid, England), ceftazidime 30μg (Becton Dickinson, USA), and aztreonam 30μg (Oxoid, England) were used in this method. Culture was incubated at 35-37ºC for 18-24 hours (Putra et al 2020;CLSI 2017). The inhibition zone that appeared on the cup after incubation was measured based on CLSI guidelines showing an inhibition zone ≤ 27 mm in cefotaxime, inhibition zone ≤ 22 mm in ceftazidime, inhibition zone ≤ 27 mm in aztreonam, and there was a synergy between cefotaxime/ceftazidime with a combination of ≤ 22 mm in ceftazidime, inhibition zone ≤ 27 mm in aztreonam, and there was a synergy between cefotaxime/ceftazidime with amoxicillin-clavulanic in the form of synergy expansion of the inhibition zone between the two disks and there is an increase in the inhibition zone ≥ 5 mm between the diameter of the cephalosporin disk and the cephalosporinclavulanate disk assert that the E. coli bacteria is positive ESBL (CLSI 2017).…”

“…Antimicrobial susceptibility and phenotypic detection of ESBL generators were used AST N280 cards (bioMérieux, Marcy-L'Étoile, France). Results were analyzed automatically by the system and interpreted as sensitive, intermediate, and resistant (Brower et al 2017;Biomerioux 2017;Putra et al 2020).…”