Detection of the Feline Leukemia Virus and other Mammalian Oncornaviruses by Immunofluorescence1

Hardy, W. D.; Hirshaut, Yashar; Hess, P

doi:10.1159/000427906

Cited by 73 publications

(61 citation statements)

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“…The immunosuppressive sequelae of viraemic FeLV infections have been shown to be responsible for the increased morbidity and mortality among FeLV-infected cats (Hardy et al, 1973;Grant et al, 1980). The majority of overtly FeLV-infected cats succumb to unrelated opportunistic infections associated with an immunosuppressed state ).…”

Section: Discussionmentioning

confidence: 99%

“…Removal of this population would necessarily leave the affected individual open to infection by opportunistic pathogens. Upon infection with FeLV, a minority of cats become persistently viraemic with low titre humoral responses to FeLV-associated antigens (Hardy et al, 1973;Grant et al, 1980). These cats are highly susceptible to opportunistic infections from the time of diagnosis to the onset of leukaemia with a majority succumbing to opportunistic infections .…”

Section: Introductionmentioning

confidence: 99%

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Suppression of in vitro Neutrophil Function by Feline Leukaemia Virus (FeLV) and Purified FeLV-p15E

Lafrado¹,

Lewis²,

Mathes

et al. 1987

Journal of General Virology

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Suppression of in vitro Neutrophil Function by Feline Leukaemia Virus (FeLV) and Purified FeLV-p15E

Lafrado¹,

Lewis²,

Mathes

et al. 1987

Journal of General Virology

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“…FeLV group-specific antigen (GSA) was detected by an indirect immunofluorescence assay modified from that developed by Hardy et al (1973). The primary reagent was goat antiserum against ether-disrupted FeLV; the antiserum was absorbed extensively with normal feline blood cells to remove heterologous and non-specific reactivity .…”

Section: Methodsmentioning

confidence: 99%

Polymorphonuclear Leukocyte Dysfunction Associated with Feline Leukaemia Virus Infection

Lewis¹,

Duska²,

Stiff³

et al. 1986

Journal of General Virology

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SUMMARYThe chemiluminescent characteristics of enriched (>95%) peripheral blood polymorphonuclear leukocyte populations (PMN) from normal and feline leukaemia virus (FeLV)-infected cats were investigated. FeLV-infected cats demonstrated a significantly lower (P < 0.001) PMN chemiluminescent response when compared to the response of normal age-matched controls. Normal PMN treated with FeLVinfected cat serum exhibited a depressed response in comparison to control cells. A titration of serum from infected cats supplemented with normal serum revealed a titratable suppression of chemiluminescence with increasing concentration of serum from the infected cats. However, PMN from FeLV-infected cats treated with normal serum displayed a slight increase in chemiluminescence over the same cells in autologous serum. The addition of inactivated FeLV to normal PMN caused a titratable decrease in chemiluminescence.

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“…No virus was discernible by this method in the control cells. c3471 cells were also strongly positive for MuLV gs-1, as well as gs-3 antigens, whereas the B559 cells were negative, as assayed by the indirect irnmunofluorescence technique by Dr. W. Hardy (12,13). Preliminary results in this laboratory indicate that the c3471 cells possess the Gross cell-surface antigen, which was not detected in the control cells.…”

Section: Methodsmentioning

confidence: 97%

Tumorigenicity, Immunogenicity, and Virus Production in Mouse Melanoma Cells Treated with 5-Bromodeoxyuridine

Silagi

Beju

Wrathall

et al. 1972

Proc. Natl. Acad. Sci. U.S.A.

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Bromodeoxyuridine (BrdU), whether administered in a 30-hr pulse of 30 Mg/ml or continuously in low concentrations (1-3 ug/ml), significantly increased production of particles with the morphology of murine leukemia virus in a mouse melanoma (B16) Reversible suppression of tumorigenic potential of mouse melanoma cells grown in culture for long periods in medium containing low concentrations of the thymidine analog 5-bromodeoxyuridine (BrdU) has been demonstrated (1, 2). Morphology of these cells changed dramatically during growth in the presence of BrdU, although the growth rate remained essentially normal when the concentration was 1 jig/ml. These effects required DNA synthesis, and BrdU was incorporated into the DNA (1, 2). Further, mice injected with a clonally derived strain grown continuously in the presence of MATERIALS AND METHODSThe pigmented clone B559 was derived (6, 7) from a melanoma (B16), which originated spontaneously in a C57BL/6 mouse (8). B559 clone was the parental line for all cells used in these experiments (Fig. 1)

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Detection of the Feline Leukemia Virus and other Mammalian Oncornaviruses by Immunofluorescence1

Cited by 73 publications

References 0 publications

Suppression of in vitro Neutrophil Function by Feline Leukaemia Virus (FeLV) and Purified FeLV-p15E

Suppression of in vitro Neutrophil Function by Feline Leukaemia Virus (FeLV) and Purified FeLV-p15E

Polymorphonuclear Leukocyte Dysfunction Associated with Feline Leukaemia Virus Infection

Tumorigenicity, Immunogenicity, and Virus Production in Mouse Melanoma Cells Treated with 5-Bromodeoxyuridine

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