1998
DOI: 10.1007/s002170050243
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Detection of the genetic modification in heat-treated products of Bt maize by polymerase chain reaction

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Cited by 116 publications
(87 citation statements)
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“…The potatoes were subjected to high temperatures (150 to 175 °C) for short times (minutes), conditions that are commonly used in drying and frying processes. Until now, DNA degradation during food production was either monitored in processes run at temperatures up to 121 °C (Bauer et al, 2003;Gryson et al, 2002;Hupfer et al, 1998Hupfer et al, , 1999Klein et al, 1998) or detected upon investigation of final products for reasons of traceability and labelling of GM food according to the European legislation (European Commission, 1997, 2000. For example, Klein et al (1998) showed a reduction of plasmid DNA in the course of sugar production by three orders of magnitude during heat treatment at 121 °C for 30 min.…”
Section: Discussionmentioning
confidence: 99%
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“…The potatoes were subjected to high temperatures (150 to 175 °C) for short times (minutes), conditions that are commonly used in drying and frying processes. Until now, DNA degradation during food production was either monitored in processes run at temperatures up to 121 °C (Bauer et al, 2003;Gryson et al, 2002;Hupfer et al, 1998Hupfer et al, , 1999Klein et al, 1998) or detected upon investigation of final products for reasons of traceability and labelling of GM food according to the European legislation (European Commission, 1997, 2000. For example, Klein et al (1998) showed a reduction of plasmid DNA in the course of sugar production by three orders of magnitude during heat treatment at 121 °C for 30 min.…”
Section: Discussionmentioning
confidence: 99%
“…Polyamines such as spermine were identified to exhibit protective effects on DNA against irradiation (Douki et al, 2000;Oh and Kim, 1998). Heat treatment and pH were identified as the main degrading parameters in food, with regard to processing condition (Bauer et al, 2003;Hupfer et al, 1998). Klein et al (1998) monitored the degradation of DNA during production of sugar by spiking experiments, and determined the overall efficacy of DNA elimination in this process to >10 14 .…”
Section: Introductionmentioning
confidence: 99%
“…Restriction analysis 29,36,[38][39][40][41] and Southern Blot 26,27,42,43 have also been used as confirmation methods.…”
Section: Confirmation Methodsmentioning
confidence: 99%
“…All food samples were tested with CP4-EPSPS, CryIAb and PAT/pat PST; the feed samples were tested with CP4-EPSPS PST from Envirologix and Neogene. DNA was extracted from homogenised material using a CTAB extraction kit [13] or commercially available DNA extraction kits (Wizard r Magnetic DNA Purification System for Food from Promega, ChargeSwith gDNA Plant 1:1,000 na na na na 10.9 (2.6) S e e d m a i z e G A 2 1 n d n a n a n a n a na Leaf soy GTS-40- Kit from Invitrogen, GeneSpin Extraction Kit from GeneScan). The extraction was performed according to the manufacturer's recommendations.…”
Section: Side-to-side Pst and Pcr Analysis Of Gm Plant-derived Matricesmentioning
confidence: 99%