Detection of the genotype and biotype variations of bovine viral diarrhea virus from persistently infected dairy cattle in Java, Indonesia

Wuryastuti, Hastari

doi:10.4172/2157-7579-c1-029

Cited by 5 publications

(11 citation statements)

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“…Classification of subgenotypes of five BVDV isolates based on the five regions, 5 UTR, NPro, NS3, NS5B, and E2, resulted in two subgenotypes BVDV1a and BVDV1c, as per previously published studies in Indone sia (Wuryastuti et al 2018). The 5'UTR phylogenetic tree using Australian BVDV reference sequences illus trated that three out of five Indonesian viruses belonged to the BVDV1c.…”

Section: Discussionsupporting

confidence: 71%

“…According to Yeşilbağ et al (2017), the comparison of global BVDV1 distribution showed a greater distribution of BVDV1b followed by BVDV1a, whereas BVDV1c was the most dominant subgenotype in Australia (Reichel et al 2018). Comparison of BVD1a subgenotypes based on 5' UTR is threefold greater than BVD1c in Java, In donesia (Wuryastuti et al 2018).…”

Section: Discussionmentioning

confidence: 96%

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Genetic recombination of bovine viral diarrhea virus subgenotype -1a and -1c in persistently infected dairy cattle

Irianingsih

Poermadjaja

Wuryastuti

et al. 2020

Indones. J. Biotechnol.

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The bovine viral diarrhea virus (BVDV) is a major viral pathogen in cattle worldwide. In Indonesia, diversity in subgenotypes of BVDV-1 has been observed, with the highest proportion of subgenotype -1a, followed by -1c, -1b, and -1d. So far, phylogenetic analysis of BVDV-1 is based on nucleotide sequences of the 5′ UTR and partial NS5B regions. Accuracy in identifying the subgenotype and antigenic type is critical for vaccine development and effective vaccination. The aim of this study was to determine genetic recombination of BVDV through phylogenetic analysis of five different regions (5′ UTR, NPro, E2, NS3, and NS5B) of BVDV in persistently infected dairy cattle. Five isolates were sequenced using next-generation sequencing, and data were analyzed with the CLC Genomic Workbench 9.0 and MEGA-X programs. Phylogenetic analysis based on the 5′ UTR (275 nt), NPro (504 nt), E2 (1,122 nt), NS3 (2,049 nt), and NS5B (2,157 nt) regions indicated that one BVDV isolate from Banyumas, Central Java, could be classified into different subgenotypes based on the E2 region (-1c), but the same subgenotype based on the other four regions (-1a), suggesting the presence of genetic recombination of the BVDV subgenotypes -1a and -1c in persistently infected dairy cattle.

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Section: Discussionsupporting

confidence: 71%

Section: Discussionmentioning

confidence: 96%

Genetic recombination of bovine viral diarrhea virus subgenotype -1a and -1c in persistently infected dairy cattle

Irianingsih

Poermadjaja

Wuryastuti

et al. 2020

Indones. J. Biotechnol.

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“…Here, BVDV-1 was detected using nested-multiplex PCR in 46 out of 61 (75%) field samples from Central Java and East Java between 2013 and 2017, but BVDV-2 was not detected from all samples tested. Based on previous studies in Indonesia, the reported BVDV genotype was BVDV-1 [25,33,34]. Based on the BVDV nucleotide sequences that were published, there are considerably more virus isolates described as BVDV-1 (88.2%) than BVDV-2 (11.8%) [35].…”

Section: Discussionmentioning

confidence: 99%

“…Based on previous studies in Indonesia, the reported BVDV genotype was BVDV-1 [25,33,34]. Based on the BVDV nucleotide sequences that were published, there are considerably more virus isolates described as BVDV-1 (88.2%) than BVDV-2 (11.8%) [35].…”

Section: Discussionmentioning

confidence: 99%

Genetic analysis of NS5B gene from bovine viral diarrhea virus-infected cattle in Central and East Java, Indonesia

et al. 2019

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Background and Aim: A previous study divided Indonesian bovine viral diarrhea virus (BVDV)-1 into subgenotypes BVDV-1a to BVDV-1d based on the partial NS5B gene using strain Bega as reference for BVDV-1a. In fact, it is clustered into BVDV-1c with strain Bega-like Australia. BVDV genotyping has been done on isolates from Jakarta, West and Central Java, but East Java isolates have not been genotyped. This study aimed to analyze genetic variability and amino acid residues in the nucleotide-binding pocket of the NS5B gene from infected cattle. Materials and Methods: Samples were obtained from the Sera Bank originating from active and passive surveillance of cattle that had been tested for BVDV antigen from 2013 to 2017. Detection of the p80 antibody and BVDV genotyping was carried out using ELISA and nested-multiplex-polymerase chain reaction (PCR), respectively. We defined 15 nested PCR products for partial sequencing of NS5B. Those field samples were selected from each location and year using proportional calculation as a representative sample. Homological and phylogenetic analyses of the partial NS5B gene were performed using BLAST and MEGA version 6. Results: Based on the phylogenetic tree analysis using 360 nucleotides as the partial NS5B gene, Indonesian BVDV-1 isolates from Central and East Java were subdivided to BVDV-1a (n=9), BVDV-1b (n=1), and BVDV-1c (n=5). In the present study, the homology of BVDV subgenotype -1a, -1b, and -1c was compared to the BVDV GenBank data and found 90-93%, 93%, and 92-95% respectively with the average pairwise distance of 0.207. A point mutation was shown at R283K of all BVDV isolates based on the sequence of three amino acid residues R283, R285, and I287 in the nucleotide-binding pocket as a part of the encoded RNA-dependent RNA polymerase. Conclusion: This study revealed the genetic variability of BVDV infecting cattle in Central Java and East Java, Indonesia, the subtypes BVDV-1a, BVDV-1b, BVDV-1c, and a point mutation at the R283K residue.

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“…Apart from the national production, Indonesia imports skimmed milk powder from abroad, including from countries such as Australia, Belgium, Canada, Denmark, France, Germany, The Netherlands and New Zealand [56]. Currently, all cattle used for dairy production in the country are of the Friesian Holstein (FH) breed, and a few studies have reported the infection with BVDV [42,43,49]. The characteristics of dairy farming in Indonesia are similar to those of beef cattle farms, where replacement heifers are purchased at auction and only 22.1% of farmers produce their own replacement heifers [38].…”

Section: Dairy Industrymentioning

confidence: 99%

The Epidemiology and Control of Bovine Viral Diarrhoea Virus in Tropical Indonesian Cattle

Nugroho

Silitonga²,

Reichel

et al. 2022

Pathogens

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This review aims to update the knowledge of the epidemiology of Bovine viral diarrhoea virus (BVDV) in Indonesia and Southeast Asia and provide a perspective on the control options for BVDV in the Indonesian cattle population in the future. Studies on BVDV in Indonesia, since its first report in that country, and the updated beef and dairy cattle industries are reviewed. In ten of 34 provinces, BVDV is endemic. The subgenotypes of BVDV-1a and BVDV-1c are predominant in Indonesian cattle. However, BVDV is currently not a priority disease to control in Indonesia. Cattle imports from Australia appear to be potentially the most significant source of transmission of BVDV into native cattle, but the control of BVDV conducted in the local quarantine facilities is currently not achieving the aim of controlling BVDV; thus, complementary measures are needed. With the small-scale nature of the vast majority of cattle breeding in the country, the control of BVDV in provinces in which cattle breeding is economically essential may need to be organised by regional and provincial governments. Gaps in our knowledge of BVDV are identified in this review, and strategies for the control of BVDV in Indonesia are discussed.

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Detection of the genotype and biotype variations of bovine viral diarrhea virus from persistently infected dairy cattle in Java, Indonesia

Cited by 5 publications

References 0 publications

Genetic recombination of bovine viral diarrhea virus subgenotype -1a and -1c in persistently infected dairy cattle

Genetic recombination of bovine viral diarrhea virus subgenotype -1a and -1c in persistently infected dairy cattle

Genetic analysis of NS5B gene from bovine viral diarrhea virus-infected cattle in Central and East Java, Indonesia

The Epidemiology and Control of Bovine Viral Diarrhoea Virus in Tropical Indonesian Cattle

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