Detection of the Ki‐67 Proliferation Associated Nuclear Epitope in Normal Canine Tissues Using the Monoclonal Antibody MIB‐1

Abstract

This report describes the immunohistochemical detection of the Ki-67 proliferation associated nuclear epitope by means of the MIB-1 monoclonal antibody (mAb) in paraffin tissue sections from nine samples of 16 tissues obtained from nine dogs. Three parameters were considered: the localization of the cells expressing the Ki-67 epitope, the cytological characteristics of the Ki-67 expression, and the proliferative activity of some of the tissues measured by means of the proliferative index (percentage of Ki-67 positive cells measured on 500 and 1000 cells). The MIB-1 mAb reacts with the nuclei of proliferating cells, as in humans. The proliferative index was far from representative, but we were able to give a range of values concerning the proliferative activity of normal tissues. This study serves as a basis for the expression of the Ki-67 antigen by normal canine tissues in order to visualize the proliferative compartments and, thus, allows its application as a proliferative marker in routine veterinarian histopathology.

Lymphocyte Proliferation in Lymphoid Organs of the Dromedary Camel using the Monoclonal Antibody MIB‐5 Against the Proliferation‐Associated Nuclear Epitope Ki‐67

“…In agreement with the studies of Fournel et al. (1994) and Laprie et al. (1998), a strong immune reaction of the MIB‐5 mAb was observed in the germinal centers of all lymphoid organs tested.…”

“…This indicates the presence of proliferating cells. In the dog, Laprie et al. (1998) did not record any expression of Ki‐67 in these compartments, which may be a species variation.…”

“…The present data show that the Ki‐67 proliferation‐associated nuclear epitope could be recognized in normal proliferating cells of camels using the MIB‐5 mAb. This epitope has been studied in several species including humans (Gerdes et al, 1983; Goldblum et al., 1993; Katoh et al., 1995), beef cattle (Wrobel et al., 1993), dogs (Falini et al., 1989; Fournel et al., 1994; Laprie et al., 1998), rabbits, swine, cats, rats, mice, chickens and pigeons (Falini et al., 1989). The present study has added camels to the species expressing Ki‐67, which could be detected using the MIB‐5 mAb.…”

“…(2000) suggested that the Ki‐67 antigen is involved in the progression of mitosis via its interaction with human kinesin‐like protein2 which interacts with the forkhead‐associated domain of Ki‐67. Ki‐67 was detected using the MIB‐1 monoclonal antibody (mAb) in humans (Goldblum et al., 1993; Katoh et al., 1995), dogs (Fournel et al., 1994; Sarli and Benazzi, 1994; Laprie et al., 1998) and beef cattle (Wrobel et al., 1993). Ki‐67 was also detected in human tissues using MIB‐5 mAb, which was also useful in detecting the Ki‐67 equivalent in primates, monkeys, cows, goats, sheep, pigs, dogs, rabbits and rats (MIB‐5 identification sheet, Dianova, Hamburg, Germany).…”

Cell proliferation during the early stages of human eye development

“…It is therefore widely used as a proliferation marker in histopathological research and practice (Kreitz et al 2000). Two distinct forms of Ki-67 appearing during the cell cycle differ in their phosphorylation and subnuclear localisation (Laprie et al 1998;MacCallum and Peter 1999;Endl and Gerdes 2000;Kreitz et al 2000;Yamaguchi et al 2002). Ki-67 was applied to detect proliferating cells in the initial phase of adult rat neurogenesis (Kee et al 2002), in the human fetal and postnatal hippocampal tissue and cerebellar cortex (brahµm et al 2001), and in a 13-week-old human retina (Yang et al 2002).…”