Detection of the pan neuronal marker PGP9.5 by immuno-histochemistry and quantitative PCR in eutopic endometrium from women with and without endometriosis

Zevallos, Hector Barrera-Villa; McKinnon, Brett; Tokushige, Natsuko; Mueller, Christoph; Fraser, Ian S.; Bersinger, Nick A.

doi:10.1007/s00404-014-3379-1

Cited by 17 publications

(13 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Other studies confirmed that the presence of nerve fibers demonstrated by immunohistochemistry was restricted to patients with endometriosis [7,15]. Additionaly, determining the density of nerve fibers can be a reliable marker for diagnosis of endometriosis [13].…”

Section: Resultsmentioning

confidence: 71%

Endometrial Nerve Fibers Detection in Women with Different Gynecological Pathologies: A Cross Sectional Study

Elbohoty¹,

Maeboud²,

Elsaid³

2016

Gynecol Obstet

View full text Add to dashboard Cite

Objective: To show the existence and density of endometrial nerve fibers in endometriosis and other gynecological pathologies and their relation to pain. Methods:This cross-sectional study involved 325 women planned to have gynecologic surgeries at Ain Shams University Maternity Hospital from December 2010 to February 2014. The diagnosis was adenomyosis, uterine fibroid, endometrial hyperplasia or carcinoma, polycystic ovarian syndrome (PCOS), ovarian tumors and endometriosis. Endometrial tissue was obtained during hysterectomy or with a curette and then immunostained with the pan neuronal marker anti PGP 9.5 to detect the endometrial nerve fibers and to quantify their density if present.Results: Endometrial nerve fibers were detected in all cases of endometriosis and 13.7% of other pathologies except PCOS (p<0.001). Pain was the only independent factor associated with the presence of endometrial nerve fibers (OR 14.5; 95%CI: 6.7-31.2). Type of pain was not related to the presence of endometrial nerve fibers (p=0.668). The nerve fiber density is strongly correlated with pain scores (r=0.479, p<0.001).

show abstract

Section: Resultsmentioning

confidence: 71%

Endometrial Nerve Fibers Detection in Women with Different Gynecological Pathologies: A Cross Sectional Study

Elbohoty¹,

Maeboud²,

Elsaid³

2016

Gynecol Obstet

View full text Add to dashboard Cite

show abstract

“…They found that the detection of nerve fibers in the eutopic endometrium with PGP 9.5 and neurofilament was not helpful in the diagnosis of endometriosis. Zevallos et al [12] studied the eutopic endometrial curettage of 29 patients: 16 women with endometriosis and 13 women without endometriosis. They did not find a significant difference in PGP 9.5 demonstrated fibers.…”

Section: Discussionmentioning

confidence: 99%

“…[8][9][10][11][12][13][14][15][16] Progesterone treatment revealed a decrease in endometrial nerve fibers, but no clinical significance was identified. [17] As a result, further studies were recommended in recent publications for these patient groups.…”

Section: Introductionmentioning

confidence: 99%

Diagnostic Value of Endometrial Nerve Endometrial Tissue of Patients with Endometriosis

Bektas¹

2018

South Clin Ist Euras

View full text Add to dashboard Cite

“…However, in women with peritoneal endometriosis no correlation was found between the presence of nerve fibers within the eutopic uterus and symptoms (Leslie et al, 2013; Zevallos et al, 2015). In another study, no statistical differences were found between eutopic uterus innervation density in women with endometriosis and pain verses other conditions, such as adenomyosis or uterine fibroids and pain (Zhang et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

Prostaglandin levels, vaginal innervation, and cyst innervation as peripheral contributors to endometriosis-associated vaginal hyperalgesia in rodents

McAllister

Giourgas

Faircloth

et al. 2016

Molecular and Cellular Endocrinology

View full text Add to dashboard Cite

Endometriosis is a painful condition characterized by growth of endometrial cysts outside the uterus. Here, we tested the hypothesis that peripheral innervation and prostaglandin levels contribute to endometriosis-associated pain. Female Sprague-Dawley rats (n=16) were surgically instrumented by transplanting uterine tissue onto mesenteric arteries within the pelvic cavity to create a model of endometriosis which forms extra-uterine endometrial cysts and vaginal hyperalgesia. Our results describe a significant positive correlation between endometriosis-induced vaginal hyperalgesia and cyst innervation density (sensory, r =0.70, p =0.003; sympathetic, r =0.55, p = 0.03), vaginal canal sympathetic innervation density (r =0.80, p =0.003), and peritoneal fluid levels of the prostaglandins PGE2 (r =0.65, p =0.01) and PGF2α (r =0.63, p =0.02). These results support the involvement of cyst innervation and prostaglandins in endometriosis-associated pain. We also describe how sympathetic innervation density of the vaginal canal is an important predictor of vaginal hyperalgesia.

show abstract

Detection of the pan neuronal marker PGP9.5 by immuno-histochemistry and quantitative PCR in eutopic endometrium from women with and without endometriosis

Cited by 17 publications

References 33 publications

Endometrial Nerve Fibers Detection in Women with Different Gynecological Pathologies: A Cross Sectional Study

Endometrial Nerve Fibers Detection in Women with Different Gynecological Pathologies: A Cross Sectional Study

Diagnostic Value of Endometrial Nerve Endometrial Tissue of Patients with Endometriosis

Prostaglandin levels, vaginal innervation, and cyst innervation as peripheral contributors to endometriosis-associated vaginal hyperalgesia in rodents

Contact Info

Product

Resources

About