Detection of the SARS-CoV-2 spike protein in saliva with Shrinky-Dink© electrodes

Zakashansky, Julia A.; Imamura, Amanda Hikari; Salgado, Darwin F; Mercieca, Heather C. Romero; Aguas, Raphael F L; Lao, Angelou M.; Pariser, Joseph J.; Arroyo‐Currás, Netzahualcóyotl; Khine, Michelle

doi:10.1101/2020.11.14.20231811

Cited by 9 publications

(9 citation statements)

References 53 publications

(60 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Then, the raw tap water is 1:1 mixed with 0.19× PBS to obtain a mixture with the conductivity of 0.141 S/m, which is as same as that of 0.1× PBS. During all the detection in this work, the solution conductivity is kept to be 0.141 S/m, which makes an equal effect on DEP by solution conductivity 28 , 29 . Then S-protein is added in this mixed background also called as “tap water”.…”

Section: Methodsmentioning

confidence: 99%

“…A SARS-CoV-2 virion binds directly with its receptor through spike (S-) protein on its surface, and the subunit of S1 serves as the receptor-binding domain 24 – 26 . As a result, S-protein (S1 subunit) is selected as a preferred biomarker for SARS-CoV-2 detection 27 – 29 . Because the S-protein existing on the periphery of the virion is exposed on the food surface independent of body infection and viral assembly, at a quantity more than viral RNA itself, it can act as a good biomarker for food contamination of SARS-CoV-2.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Real-time, selective, and low-cost detection of trace level SARS-CoV-2 spike-protein for cold-chain food quarantine

et al. 2021

View full text Add to dashboard Cite

Due to the friendly temperature for virus survival, SARS-CoV-2 is frequently found in cold-chain foods, posing a serious threat to public health. Utilizing an interdigitated microelectrode chip modified with an antibody probe and integrating dielectrophoresis enrichment with interfacial capacitance sensing, a strategy is presented for the detection of trace level spike-protein from SARS-CoV-2. It achieves a limit of detection as low as 2.29 × 10−6 ng/mL in 20 s, with a wide linear range of 10−5–10−1 ng/mL and a selectivity of 234:1. The cost for a single test can be controlled to ~1 dollar. This strategy provides a competitive solution for real-time, sensitive, selective, and large-scale application in cold-chain food quarantine.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Real-time, selective, and low-cost detection of trace level SARS-CoV-2 spike-protein for cold-chain food quarantine

et al. 2021

View full text Add to dashboard Cite

show abstract

“…Aptamers are used for biosensing with electrochemical impedance spectroscopy, colorimetric and fluorescent methods [ 15 ]. and to detect the nucleocapsid [ 16 ] and spike [ 17 ] proteins of the SARS-CoV-2 virus.…”

Section: Introductionmentioning

confidence: 99%

SARS-CoV-2 detection with aptamer-functionalized gold nanoparticles

Aithal

Mishriki

Gupta

et al. 2022

Talanta

View full text Add to dashboard Cite

A rapid detection test for SARS-CoV-2 is urgently required to monitor virus spread and containment. Here, we describe a test that uses nanoprobes, which are gold nanoparticles functionalized with an aptamer specific to the spike membrane protein of SARS-CoV-2. An enzyme-linked immunosorbent assay confirms aptamer binding with the spike protein on gold surfaces. Protein recognition occurs by adding a coagulant, where nanoprobes with no bound protein agglomerate while those with sufficient bound protein do not. Using plasmon absorbance spectra, the nanoprobes detect 16 nM and higher concentrations of spike protein in phosphate-buffered saline. The time-varying light absorbance is examined at 540 nm to determine the critical coagulant concentration required to agglomerates the nanoprobes, which depends on the protein concentration. This approach detects 3540 genome copies/μl of inactivated SARS-CoV-2.

show abstract

“…Thus, aptamers can be used in different applications for in vitro and in vivo diagnoses. Diverse techniques, such as PCR [26], electrochemical methods [27], and fluorescence approach [28], have been combined with aptasensors for analyzing biomarkers of SARS-CoV-2. However, the aforementioned techniques have some disadvantages.…”

Section: Introductionmentioning

confidence: 99%

Surface plasmon resonance aptasensor based on niobium carbide MXene quantum dots for nucleocapsid of SARS-CoV-2 detection

et al. 2021

View full text Add to dashboard Cite

A novel label-free surface plasmon resonance (SPR) aptasensor has been constructed for the detection of N-gene of SARS-CoV-2 by using thiol-modified niobium carbide MXene quantum dots (Nb 2 C-SH QDs) as the bioplatform for anchoring N-genetargeted aptamer. In the presence of SARS-CoV-2 N-gene, the immobilized aptamer strands changed their conformation to specifically bind with N-gene. It thus increased the contact area or enlarged the distance between aptamer and the SPR chip, resulting in a change of the SPR signal irradiated by the laser (He-Ne) with the wavelength (λ) of 633 nm. Nb 2 C QDs were derived from Nb 2 C MXene nanosheets via a solvothermal method, followed by functionalization with octadecanethiol through a self-assembling method. Subsequently, the gold chip for SPR measurements was modified with Nb 2 C-SH QDs via covalent binding of the Au-S bond also by self-assembling interaction. Nb 2 C-SH QDs not only resulted in high bioaffinity toward aptamer but also enhanced the SPR response. Thus, the Nb 2 C-SH QD-based SPR aptasensor had low limit of detection (LOD) of 4.9 pg mL −1 toward N-gene within the concentration range 0.05 to 100 ng mL −1 . The sensor also showed excellent selectivity in the presence of various respiratory viruses and proteins in human serum and high stability. Moreover, the Nb 2 C-SH QD-based SPR aptasensor displayed a vast practical application for the qualitative analysis of N-gene from different samples, including seawater, seafood, and human serum. Thus, this work can provide a deep insight into the construction of the aptasensor for detecting SARS-CoV-2 in complex environments.

show abstract

Detection of the SARS-CoV-2 spike protein in saliva with Shrinky-Dink© electrodes

Cited by 9 publications

References 53 publications

Real-time, selective, and low-cost detection of trace level SARS-CoV-2 spike-protein for cold-chain food quarantine

Real-time, selective, and low-cost detection of trace level SARS-CoV-2 spike-protein for cold-chain food quarantine

SARS-CoV-2 detection with aptamer-functionalized gold nanoparticles

Surface plasmon resonance aptasensor based on niobium carbide MXene quantum dots for nucleocapsid of SARS-CoV-2 detection

Contact Info

Product

Resources

About