Detection of the schistosome circulating cathodic antigen by enzyme immunoassay using biotinylated monoclonal antibodies

Jonge, Niels de; Kremsner, Peter G.; Krijger, F. W.; Schommer, G.; Ffflié, Y.E.; Kornelis, Dieuwke; Zeyl, R. J. M. Van; Dam, Govert J. van; Feldmeier, Hermann; Deelder, André M.

doi:10.1016/0035-9203(90)90094-u

Cited by 87 publications

(41 citation statements)

References 24 publications

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“…Stool examinations were repeated in the same way during follow-up investigations. To exclude false negative stool results due to day-to-day variation of egg excretion, efficacy of chemotherapy and possible reinfection were also controlled by quantification of circulating schistosome antigens in serum (Deelder et al 1989;De Jonge et al 1990). …”

Section: Parasitological and Serological Examinationmentioning

confidence: 99%

Sonographic prediction of variceal bleeding in patients with liver fibrosis due to Schistosoma mansoni

Richter

Dacal²,

Siqueira

et al. 1998

Tropical Med Int Health

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SummarySeveral studies have shown that the characteristic hepatic abnormalities induced by Schistosoma mansoni detectable by ultrasound correlate with the degree of oesophageal varices. So far the value of ultrasound for predicting variceal haemorrhage has not been assessed. Fifty Brazilian patients with schistosomal periportal fibrosis from Alagoas State, 18 of whom had already bled from oesophageal varices, were enrolled in a combined cross-sectional and longitudinal study and investigated clinically, by endoscopy and by ultrasound. Twenty-seven of the patients were monitored until another bleeding episode, death or for a minimum of 28 months. Eight of these patients could be followed up for a further three years. A sonographic score, which accounts for the degree of echogenic periportal thickening and of portal vein dilatation, was calculated for all patients. A highly significant correlation (P Ͻ 0.0001) existed between the sonographic score and the occurrence of previous variceal haemorrhage, paralleled by a similar correlation between the sonographic score and the degree of oesophageal varices (P Ͻ 0.001). In the 27 patients monitored longitudinally, the sonographic score indicated the risk of future variceal bleeding (P Ͻ 0.0001). The sonographic score reliably predicts the risk of variceal bleeding in individual patients with periportal fibrosis. Hence, the application of endoscopy, if available at all in endemic areas, may be restricted to the patients at risk of future variceal bleeding, as determined by ultrasound. Since portable devices can be carried even to remote areas, the application of the proposed score in community surveys could provide a new means for the identification of high-risk patients in S. mansoni-infected populations.keywords S. mansoni, hepatic abnormalities, oesophageal varices, ultrasound correspondence Joachim Richter,

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Section: Parasitological and Serological Examinationmentioning

confidence: 99%

Sonographic prediction of variceal bleeding in patients with liver fibrosis due to Schistosoma mansoni

Richter

Dacal²,

Siqueira

et al. 1998

Tropical Med Int Health

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“…Circulating cathodic antigen (CCA) and circulating anodic antigen (CAA) are Schistosoma markers that can be detected in the serum and urine of infected individuals, and the levels of these antigens represent sensitive and specific biomarkers for the intensity of infection (8) (9) . Furthermore, in a study performed in an endemic area in the State of Minas Gerais, 100% of the individuals evaluated 30 days after schistosomiasis treatment resulted negative for serum CCA (10) .…”

Section: Introductionmentioning

confidence: 99%

Performance of POC-CCA® in diagnosis of schistosomiasis mansoni in individuals with low parasite burden

Siqueira

Couto

Taboada

et al. 2016

Rev. Soc. Bras. Med. Trop.

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Introduction: Schistosomiasis, caused by Schistosoma mansoni, is a public health concern in Brazil. However, the most popular diagnostic method, the Kato-Katz technique, exhibits low sensitivity in low-endemicity areas. We aimed to compare the performance of an immunological assay, the point-of-care circulating cathodic antigen (POC-CCA®) test, with that of two parasitological techniques in a low-endemicity population. Methods: Our study included 141 individuals living in Estreito de Miralta, Minas Gerais, Brazil. Fecal samples were obtained from all participants and analyzed for schistosomiasis using two parasitological techniques: the Kato-Katz technique and the saline gradient technique. Additionally, POC-CCA® strips were utilized for testing urine samples. The results obtained by the different techniques were compared. Results: Analysis of two or 24 slides using the Kato-Katz technique resulted in a positivity rate of 10.6% (15/141) or 19.1% (27/141), respectively. The saline gradient technique yielded a positivity rate of 17.0% (24/141). The prevalence according to both parasitological techniques was 24.1% (34/141). The POC-CCA® test yielded a positivity rate of 22.7% (32/141); however, the positivity rate was merely 2.1% if trace results were considered negative. The agreements observed between POC-CCA® and the parasitological techniques were good (Kappa indexes > 0.64). The POC-CCA® test was more sensitive than the two-slide Kato-Katz technique (p < 0.05) in detecting cases of S. mansoni infection when trace results were considered positive. Conclusions: These findings reinforce the importance of using multiple diagnostic techniques in low-endemicity areas for effective control of disease.

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“…In the last few years, many diagnostic techniques have been developed, including ultrasonography, 4,5 detection of circulating antibodies to semi-purified or fractionated antigens, [6][7][8][9][10] and detection of parasite circulating antigens in different host body fluids. [11][12][13][14] Detection of specific antibodies to S. mansoni and S. haematobium adult worm microsomal antigens (MAMA and HAMA, respectively) was found to be 100% specific for both species when used in the FAST-ELISA and immunoblot assays for diagnosis of schistosomiasis. 10,15 However, results of antibody assays, in general, do not correlate well with worm burden, as measured by the egg output, nor do they discriminate between previous exposure and current infection.…”

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confidence: 99%

“…20 The ELISA techniques for quantitative determination of CAA and CCA were performed as described by Deelder and others 11 and De Jonge and others. 13 In the CAA assay, the anti-CAA monoclonal antibody (MAb) 120-1B10-A served both as the capture antibody and as the alkaline phosphataseconjugated second antibody. 11 Maxi-sorb microtitration plates (Nunc, Roskilde, Denmark) were coated with CAA mouse MAb (ascitic fluid diluted 1:1,000) and, after washing, blocked with 0.033% bovine serum albumin.…”

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confidence: 99%

Application of immunodiagnostic assays: detection of antibodies and circulating antigens in human schistosomiasis and correlation with clinical findings.

Al-Sherbiny

Osman²,

Hancock³

et al. 1999

The American Journal of Tropical Medicine and Hygiene

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Abstract. In an initial cross-sectional survey, serum, urine, and stool samples were collected from 370 participants representing about 10% of the population (n ϭ 4,438) in Behbeet village, 50 km south of Cairo, Egypt, an area well known to be endemic solely for Schistosoma haematobium. Diagnosis was approached in two parallel ways. The first approach, which simulated actual conditions in many endemic areas in Egypt, was based on physical examination and urine and stool microscopic analysis. The second approach was based on two advanced immunodiagnostic assay systems. One system detected antibodies to species-specific microsomal antigens, the other detected circulating schistosomal antigens. Microsomal antigens from S. haematobium and S. mansoni were used to detect antibodies in the Falcon assay screening test (FAST)-ELISA and the enzyme-linked immunoelectrotransfer blot (EITB). Circulating anodic antigen (CAA) and circulating cathodic antigen (CCA) were quantified in serum and urine samples in a sandwich ELISA using monoclonal antibodies. Parasitologically, the prevalence of S. haematobium was 7.01% in females and 25.82% in males, giving an overall prevalence of 15.8%. The combination of urine CCA and serum CAA for detecting circulating antigens and the combination of the S. haematobium adult worm microsomal antigens (HAMA) FAST-ELISA and the HAMA EITB for detecting antibodies significantly improved the sensitivity of detecting S. haematobium circulating antigens and antibodies. Also, including a medical examination as an integral part of field studies and correlating immunodiagnostic results with other clinical and investigational data allowed us to calculate an accurate estimation of S. haematobium prevalence in this area of low endemicity.

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Detection of the schistosome circulating cathodic antigen by enzyme immunoassay using biotinylated monoclonal antibodies

Cited by 87 publications

References 24 publications

Sonographic prediction of variceal bleeding in patients with liver fibrosis due to Schistosoma mansoni

Sonographic prediction of variceal bleeding in patients with liver fibrosis due to Schistosoma mansoni

Performance of POC-CCA® in diagnosis of schistosomiasis mansoni in individuals with low parasite burden

Application of immunodiagnostic assays: detection of antibodies and circulating antigens in human schistosomiasis and correlation with clinical findings.

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