Detection of Toxoplasma gondii genotypes in abortion women by RFLP-PCR in Al-Najaf Al-Ashraf province

Abstract

The study conducted on 400 aborted women and 30 healthy women as control groups. All these cases were defined as suspected with Toxoplasma gondii and examined by measuring IgG & IgM serum level when attended to Public Health Laboratory, in An-Najaf province from November 2015 to May 2016.seventy eight out of 400 (19.5 %) women were found infected with T. gondii by this test, only 23 positive samples were used in polymerase chain reaction – restriction fragment length polymorphism for detection genotypes of T. gondii strains By use three restriction enzymes MseI to nested PCR products GRA6 and BsiE1 & TaqI to nested PCR BTUB products, to detected strain types of Toxoplasma gondii. The results are : 13 of type I (60%), 6 of type II (25 %), and 4 of type III (15%). This can provide possible additional studies of high importance, as they will provide better understanding of the different strains of parasite genotypes is present in samples and how different genotypes are circulating nature. All the evidence provided might be helpful for showing the association between strains type of T. gondii and toxoplasmosis in infected women and aid in providing preventive actions.

Genotyping and DNA Sequencing of Toxoplasma gondii from Women and Domestic Chickens in the Wasit Province Iraq

“…For placental tissue the results of the present research disagreed with (1) in Iraq for detecting the T. gondii strain genotypes, by using 3 enzymes of restriction to the nested products of the PCR GRA-6 and TaqI and BsiE1 to the products of the nested PCR BTUB, for the detection of the T. gondii strain types. Results are: of type I1 3 (60%), of type II 6 (25 %), and of type III 4 (15%).…”

“…The results in the Table (1) shows the percentage of infections in the placenta and domestic chicken tissue, a result of the placental tissue is (62.5%) and for domestic chicken was (56.9%). The difference between the two infection rates was not significant, The infection rates in the brain, liver, and muscles, were (62.5%), (70.8%) and (58.3%) respectively, however, the differences among these percentages were not significant (p=0.65).as show in table (2).…”

“…Toxoplasma gondii has been defined as a very important obligate intra-cellular protozoa Parasite in the human and veterinary areas, categorized in phylum Apicomplexan, a pathogen [1] . A human may get the infection through the ingesting underdone meat that contains the bradyzoite form in the cyst of the tissue or through drinking water that is contaminated by the oocyst which is shed from the infected felines [2] .…”

Restriction fragment length polymorphism analysis of genes of virulent strain isolate of Toxoplasma gondii using enzyme DdeI

“…The GRA protein continues to be secreted along with the enlargement of the VP and the increase in T. gondii so that the gene encoding the GRA1 protein is often used as a marker for identifying the life of T. gondii both in the tachyzoite and bradyzoite phases [23]. Most investigators have used these genes for detecting strain types of T. gondii [39,40].…”

Mucin2 protein (MUC2), ghrelin (GHRL) and obestatin (OB) as physiological biomarkers in patients infected with Giardia lamblia parasite

“…This decrease may be due to the hypermotility of the intestine in humans infected with the Giardia parasite [24] . because its pathogen colonizes the small intestine and can attach to the epithelium but does not invade the mucosa [25] . In addition, gastrointestinal infections inhibited ghrelin production from gastric endocrine cells, leading to lower plasma and gastric mucosal ghrelin levels [26] .…”