Detection of triglyceride using an iridium nano-particle catalyst based amperometric biosensor

Abstract

The detection and quantification of triglyceride (TG) using an iridium nano-particle modified carbon based biosensor was successfully carried out in this study. The detection procedures were based on the electrochemical detection of enzymatically produced NADH. TG was hydrolyzed by lipase and the glycerol produced was catalytically oxidized by NAD-dependent glycerol dehydrogenase producing NADH in a solution containing NAD(+). Glyceryl tributyrate, a short chain triglyceride, was chosen as the substrate for the evaluation of this TG biosensor in bovine serum and human serum. A linear response to glyceryl tributyrate in the concentration range of 0 to 10 mM and a sensitivity of 7.5 nA mM(-1) in bovine serum and 7.0 nA mM(-1) in human serum were observed experimentally. The potential interference of species such as uric acid (UA) and ascorbic acid (AA) was assessed. The incorporation of a selected surfactant and an increase in the incubation temperature appeared to enhance the performance of this biosensor. The conditions for the determination of TG levels in bovine serum using this biosensor were optimized, with sunflower seed oil being used as an analyte to simulate the detection of TG in blood. The experimental results demonstrated that this iridium nano-particle modified working electrode based biosensor provided a relatively simple means for the accurate determination of TG in serum.

Screen-Printed Electrodes Modified with Metal Nanoparticles for Small Molecule Sensing

“…Being the first approach explored, original papers appear in the early 1990s. These publications employed conductive material attached directly to metal particles, so the modification consists of metallized carbon with platinum [8], palladium [9], or iridium [10,11] mixed with conventional inks used for screen printing to obtain second generation enzymatic sensors, where metallic particles are used as a catalyst. The main advantage of this methodology is in the fact that commercially available materials were simply mixed with printing binders and solvents, so only the mixing recipe was optimized.…”

Construction of a triglyceride amperometric biosensor based on chitosan–ZnO nanocomposite film

“…Recently considerable attention has been paid on the use of nanoparticles for improving biosensor, e.g. Prussian blue modified screen printed electrode [14], iridium nano-particle modified carbon paste electrode [15] polyaniline (PANI)/single-walled carbon nanotubes (SWCNTs) ITO coated glass plate [16].…”

Recent trends and perspectives in enzyme based biosensor development for the screening of triglycerides: a comprehensive review

“…In human sera, a linear concentration range from 0 to 10 mM for glyceryl tributyrate and high sensitivity was observed. The inclusion of a selected surfactant and raising the incubation temperature resulted in better performance of the proposed sensor [55]. In this amperometric TG biosensor, lipase was covalently immobilized onto the nanocomposite films electrochemically deposited on the surface of ITO coated glass plate.…”