Detection of Tumor Necrosis Factor α in Normal and Inflamed Human Dental Pulps

Pezelj-Ribarić, Sonja; Anić, Igor; Brekalo, Ivana; Miletić, Ivana; Hasan, Milena; Šimunović-Šoškić, Marica

doi:10.1016/s0188-4409(02)00396-x

Cited by 124 publications

(133 citation statements)

References 11 publications

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“…43,44 MMP family proteins elicit dual role in the pathogenesis of inflammation, stimulating protective innate and/or adaptive immune functions, as well as tissue destruction.…”

Section: Role Of Mmps In Pulpal Inflammationmentioning

confidence: 99%

“…59,60 MMP-2, MMP-3, MMP-8, MMP-9 and MMP-13 are the other matrix metalloproteinases that have been found in periapical lesions. 44,[61][62][63][64] In chronic apical periodontitis, MMP-8 has been immunohistochemically detected in polymorphonuclear neutrophils, macrophages and plasma cells. It is a complex process involving bacteria and their products, immunoglobulins, proinflammatory cytokines and other inflammatory mediators, which tend to affect one another.…”

Section: Role Of Mmps In Periapical Inflammationmentioning

confidence: 99%

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Role of matrix metalloproteinases in dental caries, pulp and periapical inflammation: An overview

Jain¹,

Bahuguna²

2015

Journal of Oral Biology and Craniofacial Research

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“…43,44 MMP family proteins elicit dual role in the pathogenesis of inflammation, stimulating protective innate and/or adaptive immune functions, as well as tissue destruction.…”

Section: Role Of Mmps In Pulpal Inflammationmentioning

confidence: 99%

Section: Role Of Mmps In Periapical Inflammationmentioning

confidence: 99%

Role of matrix metalloproteinases in dental caries, pulp and periapical inflammation: An overview

Jain¹,

Bahuguna²

2015

Journal of Oral Biology and Craniofacial Research

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“…As might be expected, our experiments showed that TNF‐α depressed the expression of Panx3 in a dose‐dependent manner. TNF‐α, both trauma and bacteria could induce its secretion, act as a pro‐inflammatory cytokine and aggravate inflammatory response, then ultimately initiate dental pulp inflammation and pain 10, 11, 12, 13, 24. Previous studies have reported that TNF‐α could suppress the expression of several hemichannels in various cell types 25, 26, 27.…”

Section: Discussionmentioning

confidence: 99%

“…The cases of dental pulpitis were diagnosed by endodontic specialists on the basis of established criterion before the root canal therapy 20, referring to those with a history of spontaneous, intense and prolonged pain without periodontal disease 11. Each group was used for H&E and immunohistochemical staining.…”

Section: Methodsmentioning

confidence: 99%

Pannexin3 inhibits TNF‐α‐induced inflammatory response by suppressing NF‐κB signalling pathway in human dental pulp cells

Song

Sun

Wang

et al. 2016

J Cellular Molecular Medi

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Human dental pulp cells (HDPCs) play a crucial role in dental pulp inflammation. Pannexin 3 (Panx3), a member of Panxs (Pannexins), has been recently found to be involved in inflammation. However, the mechanism of Panx3 in human dental pulp inflammation remains unclear. In this study, the role of Panx3 in inflammatory response was firstly explored, and its potential mechanism was proposed. Immunohistochemical staining showed that Panx3 levels were diminished in inflamed human and rat dental pulp tissues. In vitro, Panx3 expression was significantly down‐regulated in HDPCs following a TNF‐α challenge in a concentration‐dependent way, which reached the lowest level at 10 ng/ml of TNF‐α. Such decrease could be reversed by MG132, a proteasome inhibitor. Unlike MG132, BAY 11‐7082, a NF‐κB inhibitor, even reinforced the inhibitory effect of TNF‐α. Quantitative real‐time PCR (qRT‐PCR) and enzyme‐linked immunosorbent assay (ELISA) were used to investigate the role of Panx3 in inflammatory response of HDPCs. TNF‐α‐induced pro‐inflammatory cytokines, interleukin (IL)‐1β and IL‐6, were significantly lessened when Panx3 was overexpressed in HDPCs. Conversely, Panx3 knockdown exacerbated the expression of pro‐inflammatory cytokines. Moreover, Western blot, dual‐luciferase reporter assay, immunofluorescence staining, qRT‐PCR and ELISA results showed that Panx3 participated in dental pulp inflammation in a NF‐κB‐dependent manner. These findings suggested that Panx3 has a defensive role in dental pulp inflammation, serving as a potential target to be exploited for the intervention of human dental pulp inflammation.

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“…Dental caries often causes pulp inflammation once it reaches the dentine. In deep caries, there are many kinds of bacterial components, such as lipopolysaccharide (LPS) from gram-negative bacteria, that induce the production of proinflammatory mediators, including tumor necrosis factor (TNF)-α, interleukin (IL)-1 and transforming growth factor (TGF)-β1 [1][2][3] .…”

Section: Introductionmentioning

confidence: 99%

TNF-^|^alpha; and LPS Enhance MMP Production in Human Dental Pulp Cells of Deciduous Teeth

Kawai

Harada

Daito

et al. 2012

J. Hard Tissue Biology.

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Dental caries often causes pulp inflammation once it reaches the dentine. In deep caries, there are many kinds of bacterial components, such as lipopolysaccharide (LPS), that induce the production of proinflammatory mediators, including tumor necrosis factor (TNF)-α. Inflammation in dental pulp tissue is associated with tissue degradation, and matrix metalloproteinases (MMPs) are believed to participate in this destruction. In the present study, we examined whether TNF-α and LPS affect the production of MMP-1 and MMP-3 in human dental pulp cells (HDPCs) of deciduous teeth utilizing RT-PCR and ELISA analyses. Exposure of HDPCs to TNF-α at 10 ng/ml for 6 h significantly increased the gene expression of MMP-1 and MMP-3. MMP-1 gene expression was slightly affected by exposure to LPS, while MMP-3 gene expression was significantly elevated by LPS at 0.1, 1 and 5 µg/ml. TNF-α at 10 ng/ml and LPS at 1 and 5 µg/ml potentiated the protein production of MMP-3, but not MMP-1, in HDPCs. The results of this study suggests that the mechanisms by which TNF-α and LPS induce MMP-3 expression may be important links in the pathogenesis of inflammatory diseases and reparative dentin formation in HDPCs of deciduous teeth.

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Detection of Tumor Necrosis Factor α in Normal and Inflamed Human Dental Pulps

Cited by 124 publications

References 11 publications

Role of matrix metalloproteinases in dental caries, pulp and periapical inflammation: An overview

Role of matrix metalloproteinases in dental caries, pulp and periapical inflammation: An overview

Pannexin3 inhibits TNF‐α‐induced inflammatory response by suppressing NF‐κB signalling pathway in human dental pulp cells

TNF-^|^alpha; and LPS Enhance MMP Production in Human Dental Pulp Cells of Deciduous Teeth

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