Detection of Vascular Endothelial Growth Factor Messenger RNA and Vascular Endothelial Growth Factor-like Activity in Proliferative Diabetic Retinopathy

Malecaze, F; Clamens, Simone; Simorre-Pinatel, V; Mathis, A.; Chollet, Pascale; Favard, C.; Bayard, Françis; Plouët, Jean

doi:10.1001/archopht.1994.01090230090028

Cited by 324 publications

(141 citation statements)

References 32 publications

Supporting

Mentioning

129

Contrasting

Unclassified

Order By: Relevance

“…The vitreous concentration at which VEGF begins to induce these changes (12.5 nmol/L) is within the range of vitreous VEGF concentrations observed in human eyes with diabetic retinopathy. [15][16][17] The leukostasis observed in these studies was specific to VEGF because co-injection of a neutralizing antibody abrogated the response. Finally, these findings are consistent with our work showing VEGF-induced ICAM-1 expression in the retinal vasculature.…”

Section: Discussionmentioning

confidence: 87%

Vascular Endothelial Growth Factor (VEGF)-Induced Retinal Vascular Permeability Is Mediated by Intercellular Adhesion Molecule-1 (ICAM-1)

Miyamoto

Khosrof

Bursell

et al. 2000

The American Journal of Pathology

308

194

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 87%

Vascular Endothelial Growth Factor (VEGF)-Induced Retinal Vascular Permeability Is Mediated by Intercellular Adhesion Molecule-1 (ICAM-1)

Miyamoto

Khosrof

Bursell

et al. 2000

The American Journal of Pathology

308

194

View full text Add to dashboard Cite

“…VEGFA expression in retinal cells is increased 3-to 30-fold by hypoxia (16). VEGFA is significantly upregulated in diabetic retinopathy, particularly in retinal pigmented epithelial cells, glial cells, and vitreal fibroblasts (20). Moreover, higher plasma (21) and vitreous VEGFA (21-23) levels were found in individuals with proliferative diabetic retinopathy (PDR) when compared with nondiabetic control subjects (21) or with individuals with nonocular diseases (22).…”

Section: Research Design and Methods-a Total Of 1369 Caucasian Subjementioning

confidence: 99%

Multiple Variants in Vascular Endothelial Growth Factor (VEGFA) Are Risk Factors for Time to Severe Retinopathy in Type 1 Diabetes

et al. 2007

View full text Add to dashboard Cite

OBJECTIVE-We sought to determine if any common variants in the gene for vascular endothelial growth factor (VEGFA) are associated with long-term renal and retinal complications in type 1 diabetes.RESEARCH DESIGN AND METHODS-A total of 1,369 Caucasian subjects with type 1 diabetes from the Diabetes Control and Complications Trial (DCCT)/Epidemiology of Diabetes Interventions and Complications (EDIC) Study had an average of 17 retinal photographs and 10 renal measures over 15 years. In the DCCT/EDIC, we studied 18 single nucleotide polymorphisms (SNPs) in VEGFA that represent all linkage disequilibrium bins (pairwise r 2 Ն 0.64) and tested them for association with time to development of severe retinopathy, three or more step progression of retinopathy, clinically significant macular edema, persistent microalbuminuria, and severe nephropathy.RESULTS-In a global multi-SNP test, there was a highly significant association of VEGFA SNPs with severe retinopathy (P ϭ 6.8 ϫ 10 Ϫ5 )-the four other outcomes were all nonsignificant. In survival analyses controlling for covariate risk factors, eight SNPs showed significant association with severe retinopathy (P Ͻ 0.05). The most significant single SNP association was rs3025021 (hazard ratio 1.37 [95% CI 1.13-1.66], P ϭ 0.0017). Family-based analyses of severe retinopathy provide evidence of excess transmission of C at rs699947 (P ϭ 0.029), T at rs3025021 (P ϭ 0.013), and the C-T haplotype from both SNPs (P ϭ 0.035). Multi-SNP regression analysis including 15 SNPs, and allowing for pairwise interactions, independently selected 6 significant SNPs (P Ͻ 0.05).CONCLUSIONS-These data demonstrate that multiple VEGFA variants are associated with the development of severe retinopathy in type 1 diabetes. Diabetes

show abstract

“…VEGF has been shown to be significantly up-regulated in diabetic retinopathy, particularly in retinal pigmented epithelial cells, glial cells and vitreal fibroblasts [4]. Furthermore, there is mounting functional evidence that inhibition of angiogenesis, both non-specifically with pharmacological anti-angiogenic agents and specifically with anti-VEGF agents, ameliorates neovascularisation in well-characterised animal models of retinopathy [5].…”

Section: Introductionmentioning

confidence: 99%

Diabetic retinopathy is associated with a switch in splicing from anti- to pro-angiogenic isoforms of vascular endothelial growth factor

et al. 2005

View full text Add to dashboard Cite

show abstract

Detection of Vascular Endothelial Growth Factor Messenger RNA and Vascular Endothelial Growth Factor-like Activity in Proliferative Diabetic Retinopathy

Cited by 324 publications

References 32 publications

Vascular Endothelial Growth Factor (VEGF)-Induced Retinal Vascular Permeability Is Mediated by Intercellular Adhesion Molecule-1 (ICAM-1)

Vascular Endothelial Growth Factor (VEGF)-Induced Retinal Vascular Permeability Is Mediated by Intercellular Adhesion Molecule-1 (ICAM-1)

Multiple Variants in Vascular Endothelial Growth Factor (VEGFA) Are Risk Factors for Time to Severe Retinopathy in Type 1 Diabetes

Diabetic retinopathy is associated with a switch in splicing from anti- to pro-angiogenic isoforms of vascular endothelial growth factor

Contact Info

Product

Resources

About