Detection of Vascular Endothelial Growth Factor Based on Gold Nanoparticles and Immunoreaction Using Resonance Light Scattering

Chen, Zhanguang; Lei, Yulong; Gao, Wenhua

doi:10.1007/s11468-012-9442-5

Cited by 9 publications

(4 citation statements)

References 44 publications

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“…Although UV–vis measurements provide information regarding its photon extinction activity, decomposing a UV–vis extinction spectrum into its absorption and scattering component spectrum is difficult. Even though it has become common to refer to porphyrin RSS spectrum as its resonance light scattering (RLS), ,− this practice has unfortunately neglected the possibility of fluorescence contribution to the porphyrin RSS spectrum. ,, A series of recent studies showed that fluorescence signal can dominate the RSS spectrum obtained with fluorescence samples. − …”

Section: Introductionmentioning

confidence: 99%

Optical Properties and Kinetics: New Insights to the Porphyrin Assembly and Disassembly by Polarized Resonance Synchronous Spectroscopy

Vithanage

Zhang

2018

J. Phys. Chem. B

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With their unique photochemical properties, porphyrins have remained for decades the most interested chemicals as photonic materials for applications ranging from chemistry, biology, medicine, to photovoltaic. Porphyrins can self-assemble into higher order structures. However, information has been scant on the kinetics and structural evolution during porphyrin assembly and disassembly. Furthermore, quantitative understanding of the porphyrin optical activities is complicated by the complex interplay of photon absorption, scattering, and fluorescence emission that can concurrently occur in porphyrin samples. Using meso-tetrakis(4-sulfonatophenyl)porphyrin as the model molecule, reported herein is a combined UV-vis extinction, polarized Stokes-shifted fluorescence, and polarized resonance synchronous spectroscopic (PRS2) study of porphyrin assembly and disassembly in acidic solutions. Although porphyrin assembly and disassembly occur instantaneously upon the sample preparation, both processes last at least a few months before reaching their approximate equilibrium states. The two processes were monitored in situ by quantifying the porphyrin fluorescence and scattering depolarizations as well as its extinction, absorption, scattering, and fluorescence emission cross sections. In addition to a series of new insights to the porphyrin assembly and disassembly, the methodology described in this work opens the door for the in situ study of the structural and optical properties of photonic materials comprising molecular assembly.

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Section: Introductionmentioning

confidence: 99%

Optical Properties and Kinetics: New Insights to the Porphyrin Assembly and Disassembly by Polarized Resonance Synchronous Spectroscopy

Vithanage

Zhang

2018

J. Phys. Chem. B

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“…Taking into account of the low detection limit, wide linear range, high reproducibility, excellent specificity, good accuracy, suitable practicability and stability of the immuno sensor, it can be attempted to use for highly sensitive detection of VEGF and numerous analytes, for which antibodies are available, in clinical diagnostics. An electrochemical biosensor based on graphene oxide/ssDNA/PLLA nanoparticles~ 50 [68] The AR/H 2 O 2 -Apt-Au NPs/BiOCl nanocomposites probe~ 25,000 [69] Magnetic graphene oxide (MGO)-modified Au electrode~ 31 [70] Aptamer sandwich based chemiluminescence assay~ 1000 [71] Aptamer-gold nanoparticle assembly~ 45,000 [72] Gold nanoparticles and immunoreaction using resonance light scattering~ 60 [73] Single-step nanoplasmonic aptasensor~ 25 [74] CAT-mediated CL immunoassay based on CdTe QD/H 2 O 2 system~ 0.5 This study…”

Section: Resultsmentioning

confidence: 99%

Enhanced sensitivity of VEGF detection using catalase-mediated chemiluminescence immunoassay based on CdTe QD/H2O2 system

Ghavamipour

Rahmani

Shanehsaz³

et al. 2020

J Nanobiotechnol

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Background: Since vascular endothelial growth factor (VEGF) is a significant regulator of cancer angiogenesis, it is essential to develop a technology for its sensitive detection. Herein, we sensitized a chemiluminescence (CL) immunoassay through the combination of H 2 O 2-sensitive TGA-CdTe quantum dot (QD) as signal transduction, dextran as a cross-linker to prepare enzyme-labeled antigen and the ultrahigh bioactivity of catalase (CAT) as reporter enzyme. Results: Under the optimized experimental conditions, the chemiluminescence enzyme-linked immunosorbent assay (CL-ELISA) method can detect VEGF in the excellent linear range of 2-35,000 pg mL −1 , with a detection limit (S/N = 3) of 0.5 pg mL −1 which was approximately ten times lower than the commercial colorimetric immunoassay. This proposed method has been successfully applied to the clinical determination of VEGF in the human serum samples, and the results illustrated an excellent correlation with the conventional ELISA method (R 2 = 0.997). The suitable recovery rate of the method in the serum ranged from 97 to 107%, with a relative standard deviation of 1.2% to 13.4%. Conclusions: The novel immunoassay proposes a highly sensitive, specific, and stable method for very low levels detection of VEGF that can be used in the primary diagnosis of tumors. With the well-designed sensing platform, this approach has a broad potential to be applied for quantitative analysis of numerous disease-related protein biomarkers for which antibodies are available.

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“…Other analytical platforms proposed for the quantification of VEGF include electrical detection of VEGF using Si nanowire field effect transistors (Lee et al, 2009), rolling circle amplification (Cheng et al, 2010a(Cheng et al, , 2010bSuzuki and Yokoyama, 2011), enhanced resonance light scattering (Chen et al 2012), aptasensor based on fluorescence polarization , fluorescent peptide conjugated nanopillar chip (Suzuki and Yokoyama, 2011), porosity induced hydrogel microspheres (Mohammad Al-Ameen, 2013) and optical and electrochemical sensors based on an aptasensor (Abe et al, 2012;Cho et al, 2012;Freeman et al, 2012). However, expensive instrumentation is involved in these methods.…”

Section: Characterizationmentioning

confidence: 99%

Ultrasensitive SERS detection of VEGF based on a self-assembled Ag ornamented–AU pyramid superstructure

Zhao

et al. 2015

Biosensors and Bioelectronics

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Detection of Vascular Endothelial Growth Factor Based on Gold Nanoparticles and Immunoreaction Using Resonance Light Scattering

Cited by 9 publications

References 44 publications

Optical Properties and Kinetics: New Insights to the Porphyrin Assembly and Disassembly by Polarized Resonance Synchronous Spectroscopy

Optical Properties and Kinetics: New Insights to the Porphyrin Assembly and Disassembly by Polarized Resonance Synchronous Spectroscopy

Enhanced sensitivity of VEGF detection using catalase-mediated chemiluminescence immunoassay based on CdTe QD/H2O2 system

Ultrasensitive SERS detection of VEGF based on a self-assembled Ag ornamented–AU pyramid superstructure

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