Ultrasensitive SERS detection of VEGF based on a self-assembled Ag ornamented–AU pyramid superstructure

Zhao, Sen; Ma, Wei; Xu, Liguang; Wu, Xiaoling; Kuang, Hua; Wang, Libing; Xu, Chuanlai

doi:10.1016/j.bios.2015.01.056

Cited by 59 publications

(22 citation statements)

References 42 publications

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“…In order to test the applicability of the method described above onreal samples, we detected spiked hair extract samples. Also, the matrix effects of clenbuterol immunoassay, in which the collected hair sample might possibly contain interferents to the test result, were evaluated. Different concentrations of standard clenbuterol solution were added into hair extract.…”

Section: Resultsmentioning

confidence: 99%

Manual‐slide‐engaged paper chip for parallel SERS‐immunoassay measurement of clenbuterol from swine hair

et al. 2015

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Clenbuterol (CL), as a feed additive, has been banned in many countries due to its potential threat to human health. In detection of CL, a fast, low-cost technique with high accuracy and specificity would be ideal for its administrative on-field inspections. Among the attempts to pursue a reliable detection tool of CL, a technique that combines surface enhanced Raman spectroscopy (SERS) and immunoassay, is close to meet the requirements as above. However, multiple steps of interactions between CL analyte, antibody, and antigen are involved in this method, and under conventional setup, the operation of SERS/immunoassay were unwieldy. In this paper, to facilitate a more manageable sample manipulation for SERS-immunoassay measurement, a 3D paper chip was suggested. A switch-on-chip multilayered (abbreviated as SoCM-) microfluidic paper-based analysis device (μPad) was fabricated to provide operators with manual switches on the interactions between different microfluids. Besides, on a detection slip we made on the main body of our SoCM-μPad, antigen was anchored in pattern. With this architecture, multistep interactions between the CL analyte in swine hair extract and the SERS probe-modified antibody and antigen, were managed for on-chip SERS-immunoassay detection. This would be very attractive for fast, cheap, accurate, and on-site specific detection of CL from real samples.

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Section: Resultsmentioning

confidence: 99%

Manual‐slide‐engaged paper chip for parallel SERS‐immunoassay measurement of clenbuterol from swine hair

et al. 2015

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“…Preparation of Single‐Stranded DNA‐Modified Shell/Au NPs : Single‐stranded DNA‐modified NPs were prepared with reference to the previous article . Specifically, OTA aptamer was added to shell NP solution at a final concentration of 3 × 10 −6 m .…”

Section: Methodsmentioning

confidence: 99%

Chiral Shell Core–Satellite Nanostructures for Ultrasensitive Detection of Mycotoxin

Cai

Hao

Sun

et al. 2018

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Herein, the design of a DNA-based chiral biosensor is described utilizing the self-assembly of shell core-gold (Au) satellite nanostructures for the detection of mycotoxin, ochratoxin A (OTA). The assembly of core-satellite nanostructures based on OTA-aptamer binding exhibits a strong chiral signal with an intense circular dichroism (CD) peak. The integrity of the assembly of core-satellite nanostructures is limited to some extent in the presence of different levels of OTA. Correspondingly, the chiral intensity of assembly is weakened with increasing OTA concentrations, allowing quantitative determination of the target. The developed chiral sensor shows an excellent linear relationship between the CD signal and concentrations of OTA in the range of 0.1-5 pg mL with a limit of detection as low as 0.037 pg mL . The effectiveness of the biosensor in a sample of red wine is verified and a good recovery rate is obtained. These results suggest that the strategy has great potential for practical application.

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“…Up to now, various analytical methods have been reported in the literature for the determination of VEGF 165 , such as optical methods (Freeman et al 2012;Gohring et al 2010;Kopra et al 2014; M.A.A-Ameen and G.Ghosh 2013; Wang et al 2014a;Wang et al 2014;Al-Ameen and Ghosh 2013;Cho et al 2012;Kopra et al 2014;Urmann et al 2015), piezoelectric micro cantilever (Capobianco et al 2011), field-effect transistor (FET) Kwona et al 2010; O.S. Kwon et al 2012;Chen et al 2014a) surface plasmon resonance (SPR) Uludag and Tothill 2012;Y.Li et al 2007;Chen et al 2014b), quartz crystal microbalance (QCM) (Cabric et al 2010;Uludag and Tothill 2012), surface-enhanced Raman scattering (SERS) (Ko et al 2013;Zhao et al 2015), enzyme-linked immunosorbent assay (ELISA) (Hsu et al 2015;Yip-Schneider et al) and electrochemical aptasensors (Li et al 2007;Lin et al 2015;Lv et al 2013;May et al 2005;Sassolas et al 2009;Wang et al 2014b;Zhao et al 2011). …”

Section: Introductionmentioning

confidence: 98%

A high sensitive electrochemical aptasensor for the determination of VEGF165 in serum of lung cancer patient

Tabrizi

Shamsipur

Farzin

2015

Biosensors and Bioelectronics

112

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Ultrasensitive SERS detection of VEGF based on a self-assembled Ag ornamented–AU pyramid superstructure

Cited by 59 publications

References 42 publications

Manual‐slide‐engaged paper chip for parallel SERS‐immunoassay measurement of clenbuterol from swine hair

Manual‐slide‐engaged paper chip for parallel SERS‐immunoassay measurement of clenbuterol from swine hair

Chiral Shell Core–Satellite Nanostructures for Ultrasensitive Detection of Mycotoxin

A high sensitive electrochemical aptasensor for the determination of VEGF165 in serum of lung cancer patient

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