Detection of VDR gene ApaI and TaqI polymorphisms in patients with type 2 diabetes mellitus using PCR-RFLP method in a Turkish population

Dilmeç, Fuat; Uzer, Elmas; Akkafa, Feridun; Köse, Elif; Kuilenburg, André B. P.

doi:10.1016/j.jdiacomp.2008.12.002

Cited by 65 publications

(51 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our results are consistent with data reported by Dilmec F et al [21], who suggests that VDR plays an important role in the regulation of lipids, possibly mediated by its activity in adipocyte calcium metabolism; therefore, there would be an association between bone metabolism and energy homeostasis. The clinical relevance of the association of lipid profile and the OC is not entirely clear.…”

Section: Discussionsupporting

confidence: 93%

Polimorfizmy genu receptora witaminy D (TaqI oraz ApaI) oraz cyrkulacja osteokalcyny u pacjentów chorujących na cukrzycę typu 2 i osób zdrowych

Rivera-León

Palmeros-Sánchez

Llamas-Covarrubias

et al. 2015

Endokrynologia Polska

View full text Add to dashboard Cite

Section: Discussionsupporting

confidence: 93%

Polimorfizmy genu receptora witaminy D (TaqI oraz ApaI) oraz cyrkulacja osteokalcyny u pacjentów chorujących na cukrzycę typu 2 i osób zdrowych

Rivera-León

Palmeros-Sánchez

Llamas-Covarrubias

et al. 2015

Endokrynologia Polska

View full text Add to dashboard Cite

“…Therefore, the present study is the first to do this. In accordance with our results on allele frequencies and genotype distributions of the VDR gene TaqI and ApaI polymorphisms in patients with OA, several authors had also previously reported that allele frequencies and genotype distributions of the VDR gene TaqI and ApaI polymorphisms were not significantly different in patients with other diseases, such as diabetes mellitus, [15,16] osteoporosis, [17] chronic periodontitis, [18] and urolithiasis. [19] This was also true for postmenopausal women [20] compared with control subjects in the Turkish population.…”

Section: Discussionsupporting

confidence: 93%

An Investigation Into the Relationship Between Taq1 and Apa1 Polymorphisms of the Vitamin D Receptor Gene and the Development of Osteoarthritis

et al. 2011

View full text Add to dashboard Cite

Amaç: Bu çalışmada vitamin D reseptör (VDR) geniObjectives: This study aims to determine whether Vitamin D receptor (VDR) gene TaqI and ApaI polymorphisms are involved in the development of osteoarthritis in the Turkish population. Patients and methods:In this study, genomic DNA was isolated from 140 subjects (95 patients with osteoarthritis and 45 healthy controls). The DNA was amplified with specific primers by polymerase chain reaction and the restriction fragment length polymorphism (RFLP) technique was used to analyze the VDR gene TaqI and ApaI polymorphism genotypes. Polymerase chain reaction-RFLP products were assessed with and ultraviolet transilluminator by being exposed to agarose gel electrophoresis. Results:In terms of the genotype distributions and the allele frequencies of VDR gene TaqI and ApaI polymorphisms, there was no statistically significant difference (p>0.05) between the osteoarthritis patients and the controls. In addition, there was also no statistically significant difference between the genotypes and clinical characteristics of the patients or controls. Conclusion:In conclusion, the results of the present study suggest that VDR gene TaqI and ApaI polymorphisms are not involved in the development of osteoarthritis in the studied Turkish population.

show abstract

“…Malecki et al (2003) similarly studied the association of FokI, ApaI, BsmI, and TaqI polymorphisms of VDR gene with T2DM in a Polish population, but they too did not find any correlation between VDR gene polymorphisms and diabetes at any of the four polymorphic sites. Similarly, studies conducted by Dilmec et al (2010), did not provide any evidence for the association of VDR polymorphisms at ApaI and FokI sites with T2DM in a Turkish population.…”

Section: Discussionmentioning

confidence: 82%

“…Numerous studies have been carried out worldwide to study the genes that attribute to susceptibility to (Malecki et al, 2003;Herder et al, 2008;Wu et al, 2008;Bid et al, 2009;Dilmec et al, 2010;Anouti, 2013). Deficiency of vitamin D is a worldwide public health issue.…”

Section: Introductionmentioning

confidence: 99%

Association of Apa I polymorphism of vitamin d receptor gene with type 2 diabetes mellitus in Saudi population

Iyer¹,

New²,

Khoja³

et al. 2017

JEBAS

View full text Add to dashboard Cite

KEYWORDS VDR ApaI ABSTRACTVitamin D deficiency has been associated with the acceleration of the onset of T2DM. The biological effects of vitamin D are mediated by binding to the vitamin D receptor (VDR) which belongs to the steroid receptor superfamily. Although many polymorphisms exist in the VDR gene, their effect on VDR protein function and signalling is unknown. An association between VDR polymorphism and T2DM has been reported in some studies; however, it appears to vary across different populations around the world. Hence this study was carried out to investigate the relationship between VDR gene polymorphisms at three restriction sites ApaI, BsmI, and TaqI and the risk of T2DM in Saudi population. The volunteers were classified according to Fasting Blood Glucose (FBG) test as two groups, T2DM and normal group. The VDR gene was amplified by polymerase chain reaction (PCR). PCR products were digested using restriction enzymes: ApaI, TaqI, and BsmI and the bands were visualised by agarose gel electrophoresis and ethidium bromide dye under UV. The results of the current study showed a significant difference in genotypes and allele frequencies of the VDR gene polymorphisms at the ApaI site between T2DM patients and control groups.

show abstract

Detection of VDR gene ApaI and TaqI polymorphisms in patients with type 2 diabetes mellitus using PCR-RFLP method in a Turkish population

Cited by 65 publications

References 18 publications

Polimorfizmy genu receptora witaminy D (TaqI oraz ApaI) oraz cyrkulacja osteokalcyny u pacjentów chorujących na cukrzycę typu 2 i osób zdrowych

Polimorfizmy genu receptora witaminy D (TaqI oraz ApaI) oraz cyrkulacja osteokalcyny u pacjentów chorujących na cukrzycę typu 2 i osób zdrowych

An Investigation Into the Relationship Between Taq1 and Apa1 Polymorphisms of the Vitamin D Receptor Gene and the Development of Osteoarthritis

Association of Apa I polymorphism of vitamin d receptor gene with type 2 diabetes mellitus in Saudi population

Contact Info

Product

Resources

About