Detection of Verocytotoxigenic &amp;lt;i&amp;gt;Escherichia coli&amp;lt;/i&amp;gt; O157 Serotype in Dairy Products in  Abuja, Nigeria

Enem, Simon Ikechukwu; Oboegbulem, S. I.; Nafarnda, Wesley Daniel; Omeiza, Gabriel K.

doi:10.4236/ojvm.2015.511030

Cited by 4 publications

(8 citation statements)

References 15 publications

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“…However, no E. coli O157 was detected among SN-F E. coli isolates from chickens in Jos Plateau State, Northcentral Nigeria [17]. The absence of E. coli O157 in cattle in this study contrasts previous reports in cattle/dairy products sampled in Northcentral Nigeria in which phenotypic E. coli O157 prevalence ranged from 0.43-67.5% [13,19,20,28] and in Southsouth Nigeria where 6.94-28% E. coli O157 prevalence rate was reported [18,29]. In Southwestern Nigeria, phenotypic E. coli O157 prevalence rate reported in cattle/beef ranged from 3.9-28% [23,30].…”

Section: Discussioncontrasting

confidence: 83%

Sorbitol non-fermenting Escherichia coli and E. coli O157: prevalence and antimicrobial resistance profile of strains in slaughtered food animals in Southeast Nigeria

Anyanwu

Ugwu

Okorie-Kanu

et al. 2022

Access Microbiology

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Antimicrobial resistance (AMR) is one of the greatest global health challenges. Reliable monitoring of AMR in bacteria in food animals is critical in order to devise mitigation strategies and empiric treatment of infections associated with the organisms. The aim of this study was to isolate sorbitol non-fermenting (SN-F) Escherichia coli from food animals (broiler chickens, cattle and pigs) slaughtered at Ikpa Market, Nsukka, Enugu State, Southeast Nigeria, detect E. coli O157 and determine the in vitro antimicrobial resistance profile of the E. coli strains. A total of 388 faecal samples were collected from randomly selected broiler chickens (n=155), cattle (n=129) and pigs (n=104). Isolation of SN-F E. coli was done using tryptic soy broth and cefixime- and potassium tellurite-supplemented sorbitol MacConkey agar (CT-SMAC). SN-F E. coli strains were confirmed biochemically and E. coli O157 detected serologically using specific E. coli O157 latex agglutination test kit. The antimicrobial resistance profile of all strains was established using the disc diffusion method. Overall, 52 (13.4 %) SN-F E. coli strains were recovered from the broiler chickens (n=31), cattle (n=12) and pigs (n=9). A significant association (χ2=9.70; P <0.05) was observed between the prevalence of SN-F E. coli and animal species. E. coli O157 was detected in two of the samples, representing 0.51% of the 388 samples processed. All the E. coli strains were resistant to at least one antimicrobial agent tested and 76% were multidrug resistant (MDR). The mean multiple antibiotic resistance indices (MARI) for isolates from chickens, cattle and pig were 0.32, 0.18 and 0.23, respectively. This study showed that a considerable percentage of food animals slaughtered in Nsukka Southeastern Nigeria are potential reservoirs of multiple-drug-resistant SN-F E. coli, including E. coli O157 that could spread to humans and the environment.

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Section: Discussioncontrasting

confidence: 83%

Sorbitol non-fermenting Escherichia coli and E. coli O157: prevalence and antimicrobial resistance profile of strains in slaughtered food animals in Southeast Nigeria

Anyanwu

Ugwu

Okorie-Kanu

et al. 2022

Access Microbiology

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“…This study confirmed the presence of VTEC in raw and fermented milk in Sokoto metropolis. The prevalence of VTEC in raw milk from this study is higher than 0.9% and 2.0% found in Abuja (Enem et al, 2015) and Ogun State (Ivbade et al 2014) respectively. And lower than the 9.4% reported in Sokoto (Yakubu et al 2018).…”

Section: Discussioncontrasting

confidence: 59%

“…Cattle are known to be the major reservoirs of the organism and can actively shed the organism in faeces without manifestation of clinical signs (Leedom, 2006;Neher et al, 2015);Thus, there is high tendency of milk contamination following unsanitary collection from disease animals or herds with carrier of the organism. Predominance of stx2 (94.4%) in this study is related to the studies conducted in Ogun State Nigeria, India and Switzerland, where frequency of stx2 was more against other gene (Stephan et al, 2008;Vendramin et al, 2014;Enem et al, 2015). Higher frequencies of stx2 positive isolate can be of a public health concern as the strains that express stx2 were most likely associated with HUS in human (Enabulele & Nwankiti, 2016).…”

Section: Discussionmentioning

confidence: 51%

“…N=Z2p(1-q) /d2 (Thrushfield; 2007) Where, N=Sample Z= the standard normal deviate corresponding to a two-sided level of significant of 5% (1.96) p=Expected prevalence q= 1-prevalence d= is the desired level of precision (usually at 5% for single proportion) = 0.05 Based on previous prevalence of 9.6% for fermented milk (Ivbade et al 2014) and 7.3% for raw milk (Enem et al, 2015); the estimated sample size for fermented milk was 134 samples and for raw milk was 104 samples.…”

Section: Sample Size Determinationmentioning

confidence: 99%

“…Non ruminant animals such as pig and pigeons are also reported to becomes reservoirs of VTEC, and these may harbour and shed VTEC while asymptomatic (Ateba & Bezuidenhout., 2008). Raw milk can be contaminated with animal faeces and become good source of human infection if consumed without proper pasteurization (Enem et al, 2015). However, people are vulnerable to HUS after direct contact with faeces or consumption of animal products that are contaminated such as meat, milk and milk products (Lupindu et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

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Detection of Verocytotoxin-Producing Escherichia Coli in Raw and Fermented (Nono) Milk in Sokoto Metropolis, Sokoto State, Nigeria.

Shagari¹,

Bello²,

Lawan³

2022

Nig. Vet. J.

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Verocytotoxin-producing Escherichia coli (VTEC) also called Shiga toxin producing Escherichia coli (STEC), are food borne organisms which cause fatal disease in human. The bacteria are frequently found in cattle gastrointestinal tract with high potential of contaminating animal products such as milk, meat, and cheese. A cross-sectional study was conducted to investigate the prevalence of VTEC and to detect the presence of stx1, stx2, and eaeA genes in raw and fermented milk sold within sokoto metropolis.Milk samples were analysed using bacterial culture, biochemical test and PCR for molecular identification. Bacteriological culture and biochemical characterization produced Escherichia coli with detection rate 0f 16.0% (38/238). Molecular identification of isolates by Polymerase chain reaction revealed 47.4% (18/38) detection rate of VTEC. The total prevalence of VTEC in the study was 7.6% (18/238), with proportion of raw and fermented milk were 12.5% (13/104) and 3.7% (5/134). There was no association (P≥ 0.05) between VTEC and different milk types. Amplification of isolate by mPCR using specific primers stx1, stx2, and eaeA gene confirmed that (94.4% (17) harboured stx2 and 1 (5.6%) harboured stx1 and eaeA genes.The study established prevalence of VTEC in raw and fermented milk consumed within sokoto metropolis, and presence of stx1, stx2 and eaeA gene. These findings indicate potential faecal contamination of the milk with VTEC in raw and fermented milk. Veterinary services in the state should educate farmers on the importance of farm hygiene and enlighten the Hausa/Fulani nomad and public on the danger associated with the consumption of contaminated milk.

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Prevalence of Escherichia coli O157 isolated from marketed raw cow milk in Kwara State, Nigeria

et al. 2023

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Detection of Verocytotoxigenic <i>Escherichia coli</i> O157 Serotype in Dairy Products in Abuja, Nigeria

Cited by 4 publications

References 15 publications

Sorbitol non-fermenting Escherichia coli and E. coli O157: prevalence and antimicrobial resistance profile of strains in slaughtered food animals in Southeast Nigeria

Sorbitol non-fermenting Escherichia coli and E. coli O157: prevalence and antimicrobial resistance profile of strains in slaughtered food animals in Southeast Nigeria

Detection of Verocytotoxin-Producing Escherichia Coli in Raw and Fermented (Nono) Milk in Sokoto Metropolis, Sokoto State, Nigeria.

Prevalence of Escherichia coli O157 isolated from marketed raw cow milk in Kwara State, Nigeria

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