Detection of virulence and multidrug resistance operons in Pseudomonas aeruginosa isolated from Egyptian Baladi sheep and goat

Abstract

Background: Pseudomonas aeruginosa is a pit of an enormous group of free-living bacteria that are able to live everywhere and suggested to be the causative agent of great scope of acute and chronic animal infections. Aim: The current study was carried out to illustrate the prevalence of P. aeruginosa in small ruminants and existence of some virulence operons as well as its antimicrobial resistance. Materials and Methods: A total of 155 samples from sheep and 105 samples from goats (mouth abscesses, fecal swabs, nasal, tracheal swabs, and lung tissue) were collected for bacteriological study, existence of some virulence expression operons with the study of their sensitivity to the antimicrobials using disc diffusion and presence of mexR operon which is responsible for multidrug resistance (MDR). Results: The bacteriological examination revealed that P. aeruginosa was isolated from nine out of 155 samples from sheep (5.8%) and four isolates out of 105 samples from goat (3.8%). It is found that 12 (92.3%), 10 (76.9 %), and 8 (61.5%) of P. aeruginosa isolates harbored hemolysin phospholipase gene (pclH), gene (exoS), and enterotoxin gene (toxA), respectively. The results of antibiotic sensitivity test showed that all tested isolates were resistant to ampicillin, bacitracin, erythromycin, streptomycin, tetracycline, trimethoprim-sulfamethoxazole, and tobramycin but sensitive to ciprofloxacin and norfloxacin. The MDR (mexR) operon was existed in all isolates. Conclusion: There is a growing risk for isolation of virulent MDR P. aeruginosa from sheep and goat illness cases, and this should be regarded in the efficient control programs.

Occurrence, virulence, carbapenem resistance, susceptibility to disinfectants and public health hazard of Pseudomonas aeruginosa isolated from animals, humans and environment in intensive farms

“…The detection rate of tox A and exo S genes in P . aeruginosa from sheep (Dapgh et al, 2019) coincided with our results. The low frequency of tox A and exo S genes in P .…”

“…The frequency of toxA gene in our tested P. aeruginosa isolates from cattle, livestock drinking water and farm workers agreed with Elshafiee et al (2019). The detection rate of toxA and exoS genes in P. aeruginosa from sheep (Dapgh et al, 2019) coincided with our results. The low frequency of toxA and exoS genes in P. aeruginosa from clinically diseased cows (Younis et al, 2015) contradicted the finding of this study.…”

“…Previous studies reported nearly similar isolation rate for P . aeruginosa in cattle (Odumosu et al, 2016), feed in cattle farms (Ma et al, 2006), sheep (Dapgh et al, 2019; Ruiz‐Roldán et al, 2020) and livestock drinking water (Elshafiee et al, 2019). However, a higher frequency of P .…”

Occurrence, antimicrobial susceptibility, and pathogenic factors of Pseudomonas aeruginosa in canine clinical samples

“…The extracellular pathogenic factors play a key role in P. aeruginosa infection and the most investigated are represented by exotoxin A, phospholipase C, elastase, alkaline protease, and type III secretion system [61]. Few data are available in veterinary literature about the occurrence of these factors in P. aeruginosa clinical isolates from companion [62] and farm animals [63][64][65]. In the present study, a high occurrence was found for all the pathogenic markers that we investigated, suggesting the elevated virulence potential of the isolates, as well as reported in human clinical isolates [66][67][68].…”

Mixed infection of Mycoplasma and bacteria of the respiratory tract of sheep with reference to the histopathological picture in Sharkia Governorate

“…Pseudomonasaeruginosa induces various disease conditions in sheep and goats with respiratory illness considered as one of the major issues mainly represented by pneumonia leading to significant mortalities and increased economic losses (Dapgh et al, 2019).…”