Detection of virulence and pathogenicity genes in selected phytopathovars

Rakhashiya, Purvi M.; Patel, Pooja P.; Sheth, Bhavisha P.; Tank, Jigna G.; Thaker, Vrinda S.

doi:10.1080/03235408.2016.1157378

Cited by 5 publications

(5 citation statements)

References 12 publications

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“…Bakteri menimbulkan penyakit salah satunya karena mempunyai faktor patogenesitas dan tingkat patogenesitas dipengaruhi oleh faktor virulensi (Joko et al, 2014). Beberapa faktor penentu patogenesitas bakteri patogen tanaman antara lain sistem sekresi tipe I-VI (Rakhashiya et al, 2016), eksopolisakarida (EPS) dan lipopolisakarida (LPS) (Arwiyanto, 2015). T3SS merupakan mekanisme penting dalam patogenesitas bakteri Gram negatif patogen tanaman (Rakhashiya et al, 2016).…”

Section: Patogenisitas Bakteri Xanthomonas Oryzae Pv Oryzaeunclassified

“…Beberapa faktor penentu patogenesitas bakteri patogen tanaman antara lain sistem sekresi tipe I-VI (Rakhashiya et al, 2016), eksopolisakarida (EPS) dan lipopolisakarida (LPS) (Arwiyanto, 2015). T3SS merupakan mekanisme penting dalam patogenesitas bakteri Gram negatif patogen tanaman (Rakhashiya et al, 2016). Keberadaan salah satu atau beberapa faktor penentu patogenesitas dapat menyebabkan bakteri bersifat patogen atau deleterious terhadap inang.…”

Section: Patogenisitas Bakteri Xanthomonas Oryzae Pv Oryzaeunclassified

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Metode Penyimpanan Bakteri Xanthomonas Oryzae pv. oryzae Penyebab Penyakit Hawar Daun Bakteri Pada Tanaman Padi Menggunakan Glycerol

Sariasih

Widiantini

Widiawati

2020

JPLP

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The existence of Xanthomonas oryzae pv. oryzae, the bacteria which causes Bacterial Leaf Blight of rice, is really necessary for the laboratory to support the research activities of the phytopathology experts. Long-term preservation by using glycerol under -20°C can be conducted to preserve the culture’s availability to stay viable in the laboratory. This research aimed to determine the glycerol concentration that is able to preserve the viability and pathogenicity of Xanthomonas oryzae pv. oryzae bacteria after being preserved for 3 and 6 months. This experiments were performed using Completely Randomized Design which comprised of six treatments and four replications, they were: glycerol 50%, 40%, 30%, 20%, 10%, and control (without glycerol). The results of this study showed that viability of Xanthomonas oryzae pv. oryzae that was preserved within 20% glycerol solution for 6 months was different compared to control group. The number of Xanthomonas oryzae pv. oryzae bacteria on 20% glycerol was 4,49 x 109 CFU/ml, while on control group was 1,76 x 109 CFU/ml. 20% glycerol concentration had significant effect on the Bacterial Leaf Blight symptoms’ length after 6-months preservation that observed on the 7th day after incubation. The Bacterial Leaf Blight symptoms’ length on 20% glycerol treatment group was 46,2 mm and 22,7 mm on control group. Meanwhile for the 3 months preservation, the administration of glycerol did not show any significant effect on the Xanthomonas oryzae pv. oryzae’s viability and the Bacterial Leaf Blight symptoms’ length.

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Section: Patogenisitas Bakteri Xanthomonas Oryzae Pv Oryzaeunclassified

Metode Penyimpanan Bakteri Xanthomonas Oryzae pv. oryzae Penyebab Penyakit Hawar Daun Bakteri Pada Tanaman Padi Menggunakan Glycerol

Sariasih

Widiantini

Widiawati

2020

JPLP

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“…merupakan bakteri endofit pada kilemo (Litsea cubeba) (Ling et al, 2012). Bakteri dari famili yang sama (Brucellaceae) yaitu Ochrobactrum antropi merupakan patogen pada benih Prunus dulcis (Rakhashiya et al, 2016).…”

Section: Alcaligenes Faecalis L48unclassified

“…Beberapa faktor penentu patogenesitas bakteri patogen tanaman antara lain sistem sekresi tipe I-VI (Rakhashiya et al, 2016), eksopolisakarisa (EPS) dan lipopolisakarida (LPS) (Arwiyanto, 2014). T3SS merupakan mekanisme penting dalam patogenesitas bakteri Gram negatif patogen tanaman (Rakhashiya et al, 2016). Keberadaan salah satu atau beberapa faktor penentu patogenesitas dapat menyebabkan bakteri bersifat patogen atau deleterious terhadap inang.…”

Section: Salmonella Bongori Sl9unclassified

Deteksi Bakteri Patogen Terbawa Benih Akor (Acacia Auriculiformis A. Cunn. Ex Benth.)

Suharti

Joko

Arwiyanto

2017

JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA

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Detection of seed-borne pathogenic bacteria of northern black wattle (Acacia auriculiformis A. Cunn. ex Benth.). Intensive research of seed-borne pathogen of A. mangium and A. crassicarpa which have been established in industrial timber estate (HTI), while plantings development of northern black wattle in Indonesia 1990 have not recent been established in the 1990s. Very limited information available on northern black wattle diseases especially seed-borne diseases. The objectives of this study were to identify seed-borne pathogenic bacteria of northern black wattle and the effects on seed germination. Methods for the isolation of bacteria were by seed soaking, seed griding, blotter test, growing-on test on paper and soil. Identification of bacteria by PCR used 63F/1387R primer. The results showed that seed-borne bacteria of northern black wattle were Paenochrobactrum sp., Ralstonia sp., Burkholderia cepacia complex, Pseudomonas stutzeri, Acinetobacter sp., Alcaligenes faecalis, Salmonella bongori, Escherichia hermannii while pathogenic bacteria cause seedling leafspot were Micrococcus luteus and Burkholderia cepacia complex. Burkholderia cepacia complex, A. faecalis, Acinetobater sp., P. stutzeri, S. bongori and Ralstonia sp. reduced seed germination and increased rotten seed, suggested that they were the pathogenic bacteria of northern black wattle seed. Ralstonia sp. significantly increased the percentage of rotten seed and decreased shoot length and root length. P. stutzeri and S. bongori significantly inhibited the root growth. Paenochrobactrum sp. and E. hermannii were assumed as pathogen with weak virulence due to seed germination, the percentage of rotten seed and vigour index were relatively similar to untreated seed.

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“…Mechanisms of pathogenicity of phytopathogens are being extensively studied in Gram-negative and some Gram-positive bacteria (Baker et al , 2010; Venturi and Fuqua, 2013). These studies revealed that pathogens contain suits of genes including various transporter systems (Chen et al , 2010; Rakhashiya et al , 2016; Wilkens, 2015), secretion systems (TSS), virulence and associated metabolism (Abby et al , 2016; Chang et al , 2014; Green and Mecsas, 2016). In pathogens, from the cytoplasm through the inner membrane or the outer membrane to the extracellular space, the secretion of proteins requires dedicated types of machinery, i.e.…”

Section: Introductionmentioning

confidence: 99%

Does plant –microbe’s common Inter Simple Sequence Repeat lead to a new recombination? A novel mechanism of pathogenicity

Rakhashiya

Bhatt

Thaker

2021

Preprint

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A total of eight varieties of the mango from an orchard were studied using molecular markers to understand the host-pathogen interaction. From the infected leaves of the plant, a total of the 8 bacterial pathogens (Exiguobacterium arabatum, Pseudomonas mendocina, Pantoea dispersa, Bacillus sp. Pantoea ananatis, Micrococcous luteus, Microbacterium_sp., Enterobacter cloacae) were isolated and identified. All the host varieties of mango were distinguished for the genetic diversity using the Inter Simple Sequence Repeat (ISSR) DNA markers. This set of ISSR marker primers were also used for the mango pathogens. PCR amplification of the ISSR primers showed polymorphic and monomorphic band patterns in the host plants and in their pathogens. The monomorphic band generated by PCR amplification in the host and in the pathogen, by the common primer, is selected and used for PCR hybridization technique. PCR products obtained from the host, pathogen and hybridization were cloned, sequenced and compared. A multiple sequence alignment of these sequences revealed that the product of hybridization PCR was mixture of host and pathogen sequences. On this basis, we hypothesize a possibility for the recombination of host-microbes DNA as one of the mechanisms of pathogenicity for the plant pathogens using hybrid PCR technique. The possible mechanism of recombination for plant host and its pathogen is discussed.HighlightsInter Simple Sequence Repeat markers used to (i) Fingerprint the pathogens and their host (mango) and (ii) for study of the possibilities for the recombination as mechanism of pathogenicity.

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Detection of virulence and pathogenicity genes in selected phytopathovars

Cited by 5 publications

References 12 publications

Metode Penyimpanan Bakteri Xanthomonas Oryzae pv. oryzae Penyebab Penyakit Hawar Daun Bakteri Pada Tanaman Padi Menggunakan Glycerol

Metode Penyimpanan Bakteri Xanthomonas Oryzae pv. oryzae Penyebab Penyakit Hawar Daun Bakteri Pada Tanaman Padi Menggunakan Glycerol

Deteksi Bakteri Patogen Terbawa Benih Akor (Acacia Auriculiformis A. Cunn. Ex Benth.)

Does plant –microbe’s common Inter Simple Sequence Repeat lead to a new recombination? A novel mechanism of pathogenicity

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