Detection of Virulence Factors in Uropathogenic Escherichia Coli Isolated from Humans, Dogs and Cats in Portugal

Féria, Constança P.; Correia, José Duarte; Gonçalves, José Francisco de Carvalho; Machado, Jorge

doi:10.1007/0-306-46840-9_40

Cited by 12 publications

(9 citation statements)

References 6 publications

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“…SAT has a toxic activity against cell lines of bladder or kidney origin and, thus, may be important for pathogenesis of UTIs [99, 100]. Moreover, the cytolethal distending toxin (CDT) could be also considered as a virulence factor in UTIs caused by E. coli [101, 102]. Toll/interleukin (IL-1) receptor (TIR) domain-containing protein (Tcp) represents a novel class of virulence factors, which are able to subvert TLR signaling to gain a survival advantage during UTIs.…”

Section: Virulence Factors Of Uropathogenic E Colimentioning

confidence: 99%

Role of UropathogenicEscherichia coliVirulence Factors in Development of Urinary Tract Infection and Kidney Damage

Bień

Sokolova

Bożko

2012

International Journal of Nephrology

318

287

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UropathogenicEscherichia coli(UPEC) is a causative agent in the vast majority of urinary tract infections (UTIs), including cystitis and pyelonephritis, and infectious complications, which may result in acute renal failure in healthy individuals as well as in renal transplant patients. UPEC expresses a multitude of virulence factors to break the inertia of the mucosal barrier. In response to the breach by UPEC into the normally sterile urinary tract, host inflammatory responses are triggered leading to cytokine production, neutrophil influx, and the exfoliation of infected bladder epithelial cells. Several signaling pathways activated during UPEC infection, including the pathways known to activate the innate immune response, interact with calcium-dependent signaling pathways. Some UPEC isolates, however, might possess strategies to delay or suppress the activation of components of the innate host response in the urinary tract. Studies published in the recent past provide new information regarding how virulence factors of uropathogenicE. coliare involved in activation of the innate host response. Despite numerous host defense mechanisms, UPEC can persist within the urinary tract and may serve as a reservoir for recurrent infections and serious complications. Presentation of the molecular details of these events is essential for development of successful strategies for prevention of human UTIs and urological complications associated with UTIs.

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Section: Virulence Factors Of Uropathogenic E Colimentioning

confidence: 99%

Role of UropathogenicEscherichia coliVirulence Factors in Development of Urinary Tract Infection and Kidney Damage

Bień

Sokolova

Bożko

2012

International Journal of Nephrology

318

287

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“…One hundred and six uropathogenic E. coli strains shown to possess a P ¢mbrial operon were selected from three previously characterized strain collections [14,15]. These collections comprised 208 uropathogenic E. coli strains isolated from humans, dogs and cats with signi¢cant bacteriuria and clinical symptoms of UTI.…”

Section: Bacterial Strainsmentioning

confidence: 99%

“…The third collection comprised 25 dog and 25 cat hemolytic E. coli strains isolated at the Veterinary Microbiological Diagnostic Center of Utrecht University [15]. The detection of the P ¢mbrial operon in the 208 uropathogenic E. coli strains was done by PCR ampli¢cation of a 336-bp fragment between the papE and papF genes [14,15]. Fiftyone dog, 33 human and 22 cat papEF E. coli strains isolated from human, canine and feline patients with UTI were examined in this study (Table 1).…”

Section: Bacterial Strainsmentioning

confidence: 99%

Distribution of papG alleles among uropathogenic Escherichia coli isolated from different species

Féria¹

2001

FEMS Microbiology Letters

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The distribution of alleles I, II and III of the P adhesin gene papG among Escherichia coli isolated from urinary tract infections in humans, dogs and cats was studied by PCR. Allele I was present in 6% and 5% of the human and cat isolates. Allele II as such was present in 30% and 22%, or in association with allele III in 12% and 2% of the human and canine isolates, respectively. Allele III was present in 33% of the human strains and predominated largely over allele II in E. coli isolates from cystitis of animal origin (72% in dog and 95% in cat strains). The three different classes of the PapG adhesin have been suggested to play a role in host specificity, for example human versus canine specificity. Recent studies, however, showed papG III positive human and dog cystitis isolates to be largely indistinguishable. We found the Class II allele in animal isolates and detected for the first time in Europe the Class I allele in a different genetic background than the J96-like clonal group. Our findings show that uropathogenic E. coli isolates from different species can have the same papG alleles and thus may have zoonotic potential. ß

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“…UTI occurs not only in humans but also in companion animals such as dogs and cats, and has been associated with urogenital disease such as cystitis, nephritis, and prostatitis [25]. The main virulence determinants of UPEC isolated from dogs and cats include type 1 fimbriae ( fim ), pilus associated with pyelonephritis ( pap ), S fimbriae ( sfa ), afimbrial adhesion ( afa ), α-hemolysin ( hly ), aerobactin, cytotoxic necrotizing factor 1 ( cnf1 ), and cytolethal distending toxin ( cdt ) [7]. In particular, the combination of virulence genes that appears with high frequency includes papC , sfa , hlyA , and cnf 1 [8,27].…”

Section: Introductionmentioning

confidence: 99%

Virulence factors and genetic variability of uropathogenicEscherichia coliisolated from dogs and cats in Italy

et al. 2011

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In this study, the association between virulence genotypes and phylogenetic groups among Escherichia (E.) coli isolates obtained from pet dogs and cats with cystitis was detected, and fingerprinting methods were used to explore the relationship among strains. Forty uropathogenic E. coli (UPEC) isolated from dogs (n = 30) and cats (n = 10) in Italy were analysed by polymerase chain reaction (PCR) for the presence of virulence factors and their classification into phylogenetic groups. The same strains were characterized by repetitive extragenic palindromic (REP)- and enterobacterial repetitive intergenic consensus (ERIC)-PCR techniques. We found a high number of virulence factors such as fimbriae A, S fimbriae (sfa) and cytotoxic necrotizing factor 1 (cnf1) significantly associated with phylogenetic group B2. We demonstrated a high correlation between α-hemolysin A and pyelonephritis C, sfa, and cnf1 operons, confirming the presence of pathogenicity islands in these strains. In addition, UPEC belonging to group B2 harboured a greater number of virulence factors than strains from phylogenetic groups A, B1, and D. REP- and ERIC-PCR grouped the UPEC isolates into two major clusters, the former grouping E. coli strains belonging to phylogenetic group B2 and D, the latter grouping those belonging to groups A and B1. Given the significant genetic variability among the UPEC strains found in our study, it can be hypothesized that no specific genotype is responsible for cystitis in cats or dogs.

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Detection of Virulence Factors in Uropathogenic Escherichia Coli Isolated from Humans, Dogs and Cats in Portugal

Cited by 12 publications

References 6 publications

Role of UropathogenicEscherichia coliVirulence Factors in Development of Urinary Tract Infection and Kidney Damage

Role of UropathogenicEscherichia coliVirulence Factors in Development of Urinary Tract Infection and Kidney Damage

Distribution of papG alleles among uropathogenic Escherichia coli isolated from different species

Virulence factors and genetic variability of uropathogenicEscherichia coliisolated from dogs and cats in Italy

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