Detection of Visna Maedi virus in mesenteric lymph nodes and in other lymphoid tissues of sheep three years after respiratory infection

Abstract

Visna/Maedi virus (VMV), a small ruminant lentivirus responsible for lymphoproliferative pneumonia, encephalitis, arthritis and/or mastitis in sheep, has been detected in different non-lymphoid organs. However, only a few investigations have been carried out in lymphoid tissues. In this study, some lymphoid tissues and lymph node draining or non-draining VMV target organs from five sheep infected experimentally by the respiratory route three years previously were investigated. Archival samples of spleen, red bone marrow, caudal mediastinal lymph nodes, mammary lymph nodes, popliteal lymph nodes and mesenteric lymph nodes were tested by PCR for the presence of proviral DNA. Popliteal and mesenteric lymph node samples were tested also by immunohistochemical staining of the viral capsid antigen p28. The proviral DNA was detected by PCR in all the lymphoid tissue samples from the infected sheep. The viral antigen was stained in mononuclear cells in popliteal and mesenteric lymph nodes of the infected sheep. Although the lymph nodes draining the classical target organs seem to be more infected than the others, both the viral capsid antigen and the proviral DNA were present also in lymph nodes draining non-target organs, such as the mesenteric lymph nodes. These findings show the presence of VMV in different lymphoid tissues in the late stages of infection and suggest a potential role of these tissues as a site for viral reservoir and replication, even three years after infection.

Biological characterization of Pasteurella multocida present in the Saiga population

“…The detection of viruses, bacteria and parasites in PCR was carried out using the primers described in the literature for pasteurella [35], mycoplasma spp. [36], Clostridium toxins [37], Coxiella [38], Visna Maedi viruses [39], Akabane viruses [40], MCF viruses [41], Theileria [42], Babezii [43], Anaplasma [44].…”

Pathological, molecular, and serological study of small ruminant lentiviruses in Jordan

“…Two consequent steps of PCR reactions were performed to detect the presence of MVV proviral particles by amplifying the consistent LTR region. Primers sets and thermocycler regimen were obtained as described by [22]. Products of the second PCR reactions were electrophoresed through a 1.5% TBE diluted agarose gel for 40 min at 110V.…”

Detection of Maedi Visna Virus (MVV) in milk samples of Indian goats

“…Published PCR primers (specific LTR region) pairs for MVV external /T)CAGCTGATGCTT-3', Rin: 5'-TTGCACGGAATTAGTAACG-3') were used for the detection of MVV nucleic acid in the milk samples. The primers were expected to amplify the specific viral DNA template of sizes 276 and 199 bp for MVV -Fex & Rex and MVV -Fin & Rin, respectively(Preziuso et al, 2013) …”