Detection of water-borne E. coli O157 using the integrating waveguide biosensor

Abstract

Escherichia coli O157:H7, the most common serotype of enterohemorrhagic E. coli (EHEC), is responsible for numerous food-borne and water-borne infections worldwide. An integrating waveguide biosensor is described for the detection of water-borne E. coli O157, based on a fluorescent sandwich immunoassay performed inside a glass capillary waveguide. The genomic DNA of captured E. coli O157 cells was extracted and quantitative real-time PCR subsequently performed to assess biosensor-capture efficiency. In vitro microbial growth in capillary waveguide is also documented. The biosensor allows for quantitative detection of as few as 10 cells per capillary (0.075 ml volume) and can be used in conjunction with cell amplification, PCR and microarray technologies to positively identify a pathogen.

The Detection Method ofEscherichia coliin Water Resources: A Review

“…The demand for high throughput label-free multiplexed biosensors for biological sensing has increased in the last decades. Therefore, the circumstances have led to the development of fiber optics [38][39][40][41], planar waveguides [42][43][44] and surface plasmon resonance [45][46][47][48][49][50] in the area of optical biosensor. These techniques are modified by the interaction of bio-recognition element, such as enzymes, antibodies, antigens, or receptors of E. coli used on it to make it highly selective.…”

Bio-recognition and detection using liquid crystals

“…An Optical waveguide biosensor was used to identify as few as 10 cells of E. coli (Zhu et al, 2005). Optical waveguide sensor methods allow for a high level of accuracy and offer relatively lower detection limits.…”

Ultra-sensitive fluorescence detection using integrated waveguide technology

“…We have previously reported applications using solid-phase IWT, where analytes are captured on the inner surface of an open ended capillary tube and analyzed using an instrument called Signalyte TM (Li, 2005a). This format has been used to detect and quantify Escherichia coli O157 (Zhu et al, 2005) and Bacillus anthracis spores (Hang et al, 2008), where dection limits were 10 cells and 1000 spores, respectively. Based on these previous studies, a new generation intrument was developed to optimize assays based on liquid phase IWT, called Signalyte TM -II; the instrument is shown in Figure 2.…”