Detection of Wheat Spindle Streak Mosaic Virus by Serologically Specific Electron Microscopy

Haufler, K. Z.; Fulbright, D. W.

doi:10.1094/pd-67-988

Cited by 10 publications

(4 citation statements)

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“…3). Similar particles lengths have been reported for WSSMV pathotypes isolated in Canada, U.S.A. and Japan (HAUFLER and FULBRIGHT 1983, SLYKHUIS and POLAK 1971, USUGI and SAITO 1979. SBWMV particles, 100 nm to 300 nm long were also observed in these preparates (Fig.…”

Section: Resultssupporting

confidence: 83%

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Observations on a Mixed Soil‐Borne Wheat Mosaic Virus and Wheat Spindle Streak Mosaic Virus Infection in Durum Wheat (Triticum durum Desf.)

Rubies‐Autonell

Vallega

1987

Journal of Phytopathology

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Both Wheat Spindle Streak Mosaic Virus (WSSMV) and Soil‐borne Wheat Mosaic Virus (SBWMV) were found on durum wheat plants (Triticum durum Desf.) grown in a field near Rome (Italy). The simultaneous occurrence of these pathogens was demonstrated by host‐symptomatology, pattern of disease occurrence in the field, mechanical transmission tests, as well as by the morphology of viral particles and of ultrastructural modifications. Negatively stained preparates of diseased leaves collected in early, spring showed WSSMV particles and cytoplasmic cylindrical inclusions. SBWMV particles were found only in samples collected later in the season. Ultrathin sections of infected leaves collected in early spring showed characteristic WSSMV modifications such as pinwheels and membranous bodies, whereas samples collected later in the season contained also SBWMV‐like crystalline aggregates. WSSMV infection appeared to develop and decline earlier than SBWMV in the leaves of durum wheat plants infected by both, viruses. WSSMV had not been reported in Italy before.

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Section: Resultssupporting

confidence: 83%

“…WSSMV also includes streaks and dashes, but these generally become spindle-shaped. WSSMV particles are filamentous, frequently exceeding 2000 nm in length (HAUFLER andFULBRIGHT 1983, SLYKHUIS 1976). Usually, pinwheels and membranous bodies are observed in the mesophyll cells (HOOPER and WIESE 1972).…”

mentioning

confidence: 99%

Observations on a Mixed Soil‐Borne Wheat Mosaic Virus and Wheat Spindle Streak Mosaic Virus Infection in Durum Wheat (Triticum durum Desf.)

Rubies‐Autonell

Vallega

1987

Journal of Phytopathology

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“…For the latter, however, only the cited authors report the mentioned length distribution. In another study (HAUFLER and FULBRIGHT 1983) a modal length of WSSMV was determined to 1775 nm. The preparations for the latter measurements were obtained after grinding of leaves in liquid nitrogen.…”

Section: Discussionmentioning

confidence: 99%

Barley Yellow Mosaic Virus: Purification, Electron Microscopy, Serology, and other Properties of two Types of the Virus

Huth

Lesemann

Paul

1984

Journal of Phytopathology

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From naturally infected barley plants two types of barley yellow mosaic viruses have been isolated in Federal Republic of Germany. Both are identical in morphology, showing a bimodal length distribution (270–289 nm and 568–600 nm), and in symptomatology. Both induce conspicuous cytoplasmic inclusions of the pinwheel type and laminated aggregates, as well as threedimensional crystal‐like arrays of membrane material. The types differ, however, in buoyant density, serology, and transmissibility. One is transmissible by soil as well as mechanically (BaYMV‐M), and does not react with a Japanese antiserum to the Japanese virus (BaYMV‐J). The other type (BaYMV‐NM) is only transmissible by soil and reacts with BaYMV‐J‐antiserum. From mechanically infected plants BaYMV‐M was purified, and an antiserum was produced, from soil‐infected plants only mixtured BaYMV‐NM and ‐M could be obtained. BaYMV‐NM prevailed during winter, but with rising temperatures in spring BaYMV‐M was predominant. BaYMV‐M and the ‐M‐NM mixture had each two species of nucleic acids (2.7–2.8 × 106 and 1.4–1.5 × 106 d) and two major protein subunit bands were found in SDS‐PAGE (35–36 × 103 and c. 29 × 103 d).

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“…Following centrifugation (10 min, 4°C, 4,300 ϫ g), the aqueous phase was recovered, adjusted to 8% polyethylene glycol (PEG) and 0.2 M NaCl, and incubated at 0°C for 1 h. PEG precipitants were concentrated by centrifugation (10 min, 4°C, 7,700 ϫ g) and resuspended in 1 ml 0.01 M phosphate buffer. Following centrifugation (10 min, 4°C, 9,300 ϫ g), the supernatant containing partially purified virions in suspension was retained for serologically specific electron microscopic (SSEM) analysis (23,35).…”

Section: Construction Of Chimeric Wsmv Genomesmentioning

confidence: 99%

Plant Virus HC-Pro Is a Determinant of Eriophyid Mite Transmission

Stenger

Hein²,

Gildow

et al. 2005

J Virol

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Detection of Wheat Spindle Streak Mosaic Virus by Serologically Specific Electron Microscopy

Cited by 10 publications

References 0 publications

Observations on a Mixed Soil‐Borne Wheat Mosaic Virus and Wheat Spindle Streak Mosaic Virus Infection in Durum Wheat (Triticum durum Desf.)

Observations on a Mixed Soil‐Borne Wheat Mosaic Virus and Wheat Spindle Streak Mosaic Virus Infection in Durum Wheat (Triticum durum Desf.)

Barley Yellow Mosaic Virus: Purification, Electron Microscopy, Serology, and other Properties of two Types of the Virus

Plant Virus HC-Pro Is a Determinant of Eriophyid Mite Transmission

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