Plant Virus HC-Pro Is a Determinant of Eriophyid Mite Transmission

Stenger, Drake C.; Hein, Gary L.; Gildow, F. E.; Horken, Kempton M.; French, Ron

doi:10.1128/jvi.79.14.9054-9061.2005

Cited by 59 publications

(39 citation statements)

References 49 publications

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“…Choi et al (2001) reported 97.6% nucleotide and 98.7% amino acid similarity between Sidney 81 and Type, with amino acid substitutions distributed along the entire genome, though variability mostly occurred in the P1, HC-Pro, and coat protein cistrons. Virus transmission by WCM was abolished by replacement of WSMV Sidney 81 HC-Pro with homologs of Turnip mosaic virus, Agropyron mosaic virus, and Oat necrotic mottle virus (ONMV; 72.9% nucleotide and 80% amino acid identity to WSMV) (Stenger et al 2005). WSMV Sidney 81 chimeras with ONMV partial genome replacements in the 5¢-proximal half of HC-Pro failed to be transmitted by WCM.…”

Section: Discussionmentioning

confidence: 96%

“…The differences in transmission of Sidney 81 and Type could be associated with genetic variability in the helper component proteinase (HC-Pro) region, which is the determinant of WSMV transmission by WCM (Stenger et al 2005). Choi et al (2001) reported 97.6% nucleotide and 98.7% amino acid similarity between Sidney 81 and Type, with amino acid substitutions distributed along the entire genome, though variability mostly occurred in the P1, HC-Pro, and coat protein cistrons.…”

Section: Discussionmentioning

confidence: 99%

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Differential Transmission of Two Isolates ofWheat streak mosaic virusby Five Wheat Curl Mite Populations

Wosula¹,

McMechan²,

Oliveira-Hofman³

et al. 2016

Plant Disease

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Wheat streak mosaic virus (WSMV), type member of the genus Tritimovirus in the family Potyviridae, is an economically important virus causing annual average yield losses of approximately 2 to 3% in winter wheat across the Great Plains. The wheat curl mite (WCM), Aceria tosichella, transmits WSMV along with two other viruses found throughout the Great Plains of the United States. Two common genotypes of WSMV (Sidney 81 and Type) in the United States share 97.6% nucleotide sequence identity but their transmission relationships with the WCM are unknown. The objective of this study was to determine transmission of these two isolates of WSMV by five WCM populations (‘Nebraska’, ‘Montana’, ‘South Dakota’, ‘Type 1’, and ‘Type 2’). Nonviruliferous mites from each population were reared on wheat source plants mechanically inoculated with either Sidney 81 or Type WSMV isolates. For each source plant, individual mites were transferred to 10 separate test plants and virus transmission was determined by a double-antibody sandwich enzyme-linked immunosorbent assay. Source plants were replicated nine times for each treatment (90 individual mite transfers). Results indicate that three mite populations transmitted Sidney 81 at higher rates compared with Type. Two mite populations (Nebraska and Type 2) transmitted Sidney 81 and Type at higher rates compared with the other three populations. Results from this study demonstrate that interactions between virus isolates and mite populations influence the epidemiology of WSMV.

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Section: Discussionmentioning

confidence: 96%

Section: Discussionmentioning

confidence: 99%

Differential Transmission of Two Isolates ofWheat streak mosaic virusby Five Wheat Curl Mite Populations

Wosula¹,

McMechan²,

Oliveira-Hofman³

et al. 2016

Plant Disease

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“…Whereas viruses of the genus Potyvirus are transmitted non-persistently by aphids, WSMV is transmitted semi-persistently by the wheat curl mite (Aceria tosichella Keifer). Both tritimoviruses [14,17] and potyviruses [7,19] encode HC-Pro homologues required for vector transmission. Although HC-Pro sequence conservation among the two genera is limited [6], functional domains of WSMV HC-Pro [16,18] involved in vector transmission (amino-proximal) and polyprotein maturation (carboxy-proximal) are positioned similarly to that of potyvirus HC-Pro.…”

Section: Wheat Streak Mosaic Virus (Wsmv) Is the Type Species Of The mentioning

confidence: 99%

“…Wheat curl mite transmission assays were conducted essentially as described [17]. Wheat seedlings to be used as source plants for vector transmission assays were inoculated with transcripts derived from each of the ten mutant constructs.…”

Section: Wheat Streak Mosaic Virus (Wsmv) Is the Type Species Of The mentioning

confidence: 99%

Substitution of conserved cysteine residues in wheat streak mosaic virus HC-Pro abolishes virus transmission by the wheat curl mite

Young

Hein²,

French

et al. 2007

Arch Virol

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“…The polyprotein is cleaved into at least ten mature proteins by the three virus-encoded proteinases P1, HC-Pro (helper component proteinase), and NIa-Pro. WSMV is transmitted by the wheat curl mite (Aceria tosichella Keifer) (Slykhuis 1955), and both HC-Pro and coat protein (CP) have been implicated as viral determinants for mite transmission (Stenger et al 2005a(Stenger et al , 2006S. Tatineni unpublished data).…”

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confidence: 99%

Wheat streak mosaic virus Coat Protein Deletion Mutants Elicit More Severe Symptoms Than Wild-Type Virus in Multiple Cereal Hosts

Satyanarayana

Elowsky

Graybosch

2017

MPMI

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Previously, we reported that coat protein (CP) of Wheat streak mosaic virus (WSMV) (genus Tritimovirus, family Potyviridae) tolerates deletion of amino acids 36 to 84 for efficient systemic infection of wheat. In this study, we demonstrated that WSMV mutants with deletion of CP amino acids 58 to 84 but not of 36 to 57 induced severe chlorotic streaks and spots, followed by acute chlorosis in wheat, maize, barley, and rye compared with mild to moderate chlorotic streaks and mosaic symptoms by wild-type virus. Deletion of CP amino acids 58 to 84 from the WSMV genome accelerated cell-to-cell movement, with increased accumulation of genomic RNAs and CP, compared with the wild-type virus. Microscopic examination of wheat tissues infected by green fluorescent protein–tagged mutants revealed that infection by mutants lacking CP amino acids 58 to 84 caused degradation of chloroplasts, resulting in acute macroscopic chlorosis. The profile of CP-specific proteins was altered in wheat infected by mutants causing acute chlorosis, compared with mutants eliciting wild-type symptoms. All deletion mutants accumulated CP-specific major protein similarly to that in wild-type virus; however, mutants that elicit acute chlorosis failed to accumulate a 31-kDa minor protein compared with wild-type virus or mutants lacking amino acids 36 to 57. Taken together, these data suggest that deletion of CP amino acids 58 to 84 from the WSMV genome enhanced accumulation of CP and genomic RNA, altered CP-specific protein profiles, and caused severe symptom phenotypes in multiple cereal hosts.

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Plant Virus HC-Pro Is a Determinant of Eriophyid Mite Transmission

Cited by 59 publications

References 49 publications

Differential Transmission of Two Isolates ofWheat streak mosaic virusby Five Wheat Curl Mite Populations

Differential Transmission of Two Isolates ofWheat streak mosaic virusby Five Wheat Curl Mite Populations

Substitution of conserved cysteine residues in wheat streak mosaic virus HC-Pro abolishes virus transmission by the wheat curl mite

Wheat streak mosaic virus Coat Protein Deletion Mutants Elicit More Severe Symptoms Than Wild-Type Virus in Multiple Cereal Hosts

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