Detection of Whole-Genome Resequencing-Based QTLs Associated with Pre-Harvest Sprouting in Rice (<i>Oryza sativa</i> L.)

Jang, Seong-Ho; Lar, San Mar; Zhang, Hongjia; Lee, Ah-Rim; Lee, Ja-Hong; Kim, Na‐Eun; Park, So‐Yeon; Lee, Joohyun; Ham, Tae-Ho; Kwon, Soon-Wook

doi:10.9787/pbb.2020.8.4.396

Cited by 7 publications

(2 citation statements)

References 39 publications

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“…Recently, ve QTLs were detected using a recombinant inbred line (RILs) derived from a japonica cross on chromosome 3, 4, and 11 (Cheon et al 2020). qPHS1-1 and qPHS1-2 were identi ed repeatedly within the same regions on chromosome 1, under both growth chamber and eld conditions (Jang et al 2020). In addition, qSDR9.1 and qSDR9.2 on chromosome 9 were identi ed using advanced backcross lines with SSR markers (Mizuno et al 2018).…”

Section: Introductionmentioning

confidence: 99%

QTL mapping and improvement of pre-harvest sprouting (PHS) resistance using japonica weedy rice

Lee

Park

Baek

et al. 2022

Preprint

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Background The stability of cultivation and production in terms of crop yield has been threatened by climate change due to global warming. Pre-harvest sprouting (PHS) is a threat to crops, especially staple foods, including rice, because of reductions in yield and quality. To address the problem of precocious germination before harvest, we performed quantitative trait loci (QTL) analysis for PHS using F8 RILs populations derived from japonica weedy rice in Korea. Results QTL analysis revealed that two stable QTLs, qPH7 and qPH2, associated with PHS resistance were identified on chromosomes 7 and 2, respectively, explaining approximately 38% of the phenotypic variation. The QTL effect in the tested lines significantly decreased the degree of PHS, based on the number of QTLs included. Through fine mapping for main QTL qPH7, the region for the PHS was found to be anchored within 23.575–23.785 Mbp on chromosome 7 using 13 cleaved amplified sequence (CAPS) markers. Among 15 open reading frames (ORFs) within the detected region, one ORF, Os07g0584366, exhibited upregulated expression in the resistant donor, which was approximately nine times higher than that of susceptible japonica cultivars under PHS-inducing conditions. Conclusion A QTLs introgression japonica line was developed to improve the characteristics of PHS and design practical PCR-based DNA markers for marker-assisted backcrosses of many other PHS-susceptible japonica cultivars.

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Section: Introductionmentioning

confidence: 99%

QTL mapping and improvement of pre-harvest sprouting (PHS) resistance using japonica weedy rice

Lee

Park

Baek

et al. 2022

Preprint

View full text Add to dashboard Cite

show abstract

“…Recently, five QTLs were detected using a recombinant inbred line (RILs) derived from a japonica cross on chromosome 3, 4, and 11 ( Cheon et al., 2020 ). qPHS1-1 and qPHS1-2 were identified repeatedly within the same regions on chromosome 1, under both growth chamber and field conditions ( Jang et al., 2020 ). In addition, qSDR9.1 and qSDR9.2 on chromosome 9 were identified using advanced backcross lines with SSR markers ( Mizuno et al., 2018 ).…”

Section: Introductionmentioning

confidence: 99%

QTL mapping and improvement of pre-harvest sprouting resistance using japonica weedy rice

et al. 2023

View full text Add to dashboard Cite

The stability of cultivation and production in terms of crop yield has been threatened by climate change due to global warming. Pre-harvest sprouting (PHS) is a threat to crops, especially staple foods, including rice, because of reductions in yield and quality. To address the problem of precocious germination before harvest, we performed quantitative trait loci (QTL) analysis for PHS using F8 RILs populations derived from japonica weedy rice in Korea. QTL analysis revealed that two stable QTLs, qPH7 and qPH2, associated with PHS resistance were identified on chromosomes 7 and 2, respectively, explaining approximately 38% of the phenotypic variation. The QTL effect in the tested lines significantly decreased the degree of PHS, based on the number of QTLs included. Through fine mapping for main QTL qPH7, the region for the PHS was found to be anchored within 23.575–23.785 Mbp on chromosome 7 using 13 cleaved amplified sequence (CAPS) markers. Among 15 open reading frames (ORFs) within the detected region, one ORF, Os07g0584366, exhibited upregulated expression in the resistant donor, which was approximately nine times higher than that of susceptible japonica cultivars under PHS-inducing conditions. Japonica lines with QTLs related to PHS resistance were developed to improve the characteristics of PHS and design practical PCR-based DNA markers for marker-assisted backcrosses of many other PHS-susceptible japonica cultivars.

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Application of CRISPR/Cas9 Genome Editing System to Reduce the Pre- and Post-Harvest Yield Losses in Cereals

Krishna¹,

Maharajan²,

Ceasar³

2022

TOBIOTJ

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Cereals are an important source of food for millions of people across low-middle-income countries. Cereals are considered a staple food for poor people. The majority of the people are depending upon agricultural occupation. Agriculture provides a primary source of income for many farmers in low-middle-income countries. The pre- and post-harvest loss of crop yield affects farmers and is a major problem in achieving food security. Biotic and abiotic factors cause pre- and post-harvest loss of crop yield worldwide. It significantly affects the economic status of farmers as well as low-middle-income countries. Many advanced technologies are available for resolving the pre- and post-harvest loss of crop yield. The past few decades have seen remarkable progress in crop improvement. Especially high-throughput genome sequencing approaches contributed to advancement in the crop improvement. Genome-editing has also been considered a key tool for crop improvement. The clustered, regularly interspaced, short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) system has become a potent genome editing system for modifying key traits in cereal crops. CRISPR/Cas9 system offers new opportunities for addressing pre-and post-harvest constraints affecting cereal grain production and storage. In this review, we discuss the application of the CRISPR/Cas9 genome editing system to reduce pre-and post-harvest yield loss in cereal crops. It may promote the economic status of farmers and reduce food demand in the coming decades.

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Detection of Whole-Genome Resequencing-Based QTLs Associated with Pre-Harvest Sprouting in Rice (Oryza sativa L.)

Cited by 7 publications

References 39 publications

QTL mapping and improvement of pre-harvest sprouting (PHS) resistance using japonica weedy rice

QTL mapping and improvement of pre-harvest sprouting (PHS) resistance using japonica weedy rice

QTL mapping and improvement of pre-harvest sprouting resistance using japonica weedy rice

Application of CRISPR/Cas9 Genome Editing System to Reduce the Pre- and Post-Harvest Yield Losses in Cereals

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