Detection of β-glucosidase as saprotrophic ability from an ectomycorrhizal mushroom, Tricholoma matsutake

Kusuda, Mizuho; Ueda, Mitsuhiro; Nakazawa, Masami; Miyatake, Kazutaka; Konishi, Yasuhito; Araki, Yoshihito; Terashita, Takao; Yamanaka, Katsuji

doi:10.1007/s10267-005-0289-x

Cited by 29 publications

(32 citation statements)

References 13 publications

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“…This study detected significantly higher activities of ␤-glucosidase and xylosidase in Shiro ϩ soil than in nearby Shiro Ϫ soil sampled immediately after sporocarps were harvested. Many root tips in Shiro ϩ soil showed signs of necrosis, which is a typical response of the host plant to colonization by T. matsutake (8,26) (45) showed that T. matsutake mycelium invaded the xylem cell walls of pine sawdust and how this fungus was able to produce ␤-glucosidase when pine bark was used as a substrate in vitro (19,44). Within Shiro ϩ soil, the products degraded from cellulose or hemicellulose by matsutake itself and other Shiro ϩ microbes would be important carbon sources for subsequent growth.…”

Section: Discussionmentioning

confidence: 99%

“…Hemicellulotic carbohydrates decrease rapidly during the early stages of decomposition (35). Moreover, matsutake mycelium produces ␤-glucosidase, an enzyme that hydrolyzes oligosaccharides having a ␤-1,4 linkage when incubated on pine bark or even in pure cultures (19,44). These in vitro studies indicate that T. matsutake is able to directly degrade and mobilize certain sources of organic carbon.…”

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confidence: 91%

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Tricholoma matsutake Dominates Diverse Microbial Communities in Different Forest Soils

Vaario¹,

Fritze²,

Spetz³

et al. 2011

Appl Environ Microbiol

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Fungal and actinobacterial communities were analyzed together with soil chemistry and enzyme activities in order to profile the microbial diversity associated with the economically important mushroom Tricholoma matsutake. Samples of mycelium-soil aggregation (shiro) were collected from three experimental sites where sporocarps naturally formed. PCR was used to confirm the presence and absence of matsutake in soil samples. PCR-denaturing gradient gel electrophoresis (DGGE) fingerprinting and direct sequencing were used to identify fungi and actinobacteria in the mineral and organic soil layers separately. Soil enzyme activities and hemicellulotic carbohydrates were analyzed in a productive experimental site. Soil chemistry was investigated in both organic and mineral soil layers at all three experimental sites. Matsutake dominated in the shiro but also coexisted with a high diversity of fungi and actinobacteria. Tomentollopsis sp. in the organic layer above the shiro and Piloderma sp. in the shiro correlated positively with the presence of T. matsutake in all experimental sites. A Thermomonosporaceae bacterium and Nocardia sp. correlated positively with the presence of T. matsutake, and Streptomyces sp. was a common cohabitant in the shiro, although these operational taxonomic units (OTUs) did not occur at all sites. Significantly higher enzyme activity levels were detected in shiro soil. These enzymes are involved in the mobilization of carbon from organic matter decomposition. Matsutake was not associated with a particular soil chemistry compared to that of nearby sites where the fungus does not occur. The presence of a significant hemicellulose pool and the enzymes to degrade it indicates the potential for obtaining carbon from the soil rather than tree roots.

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 91%

Tricholoma matsutake Dominates Diverse Microbial Communities in Different Forest Soils

Vaario¹,

Fritze²,

Spetz³

et al. 2011

Appl Environ Microbiol

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“…Then, we studied carbohydrase production from T. matsutake on a partially modifi ed matsutake liquid medium (PMML medium) and showed that T. matsutake produced α-amylase, α-glucosidase, and potent β-glucosidase activity in static culture fi ltrate. The three kinds of enzymes from T. matsutake were already purifi ed (α-glucosidase is a partially purifi ed enzyme), and their enzymatic properties were characterized (Kusuda et al 2003(Kusuda et al , 2006. These results suggest that T. matsutake has saprotrophic abilities (Kusuda et al 2006).…”

Section: Introductionmentioning

confidence: 94%

“…The three kinds of enzymes from T. matsutake were already purifi ed (α-glucosidase is a partially purifi ed enzyme), and their enzymatic properties were characterized (Kusuda et al 2003(Kusuda et al , 2006. These results suggest that T. matsutake has saprotrophic abilities (Kusuda et al 2006).…”

Section: Introductionmentioning

confidence: 94%

Effects of carbohydrate substrate on the vegetative mycelial growth of an ectomycorrhizal mushroom, Tricholoma matsutake, isolated from Quercus

et al. 2007

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We studied the characteristics of the utilization of carbohydrate substrates and the production of those hydrolyzing enzymes of the Tricholoma matsutake J-1 strain isolated from hardwood (Quercus sp.). In the culture medium, 5% glucose inhibited mycelial growth. The growth inhibition rate was remarkable in the Z-1 strain from softwood (Pinus densifl ora) compared with that of the J-1 strain from hardwood. α-Amylase production varied with starches from different origins in contrast to mycelial growth. The range of the effect of 0.5%-15% soluble starch on vegetative mycelial growth was also investigated. The optimal concentration for mycelial growth was 15% for the J-1 strain but 10% for the Z-1 strain. Mycelial growth of the J-1 strain was strongly inhibited in PMML medium containing Sunpeal-CP prepared from sulfi te pulp softwood waste, but that of the Z-1 strain was not inhibited by Sunpeal-CP. Moreover, mycelial growth of the J-1 strain from Quercus sp. dramatically decreased with the addition of CNF-HWSF (hot watersoluble fractions from corn fi ber) to the PMML and PDL medium. However, inhibition by CNF-HWSF was not shown in the Z-1 strain from P. densifl ora.

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“…Courty et al 2005Courty et al , 2006 impart the ability to degrade complex ligno-cellulosic compounds. For example, Tricholoma matsutake is able to secrete -glucosidase, which hydrolyses oligosaccharides having a -1,4-linkage such as cellobiose, but is not able to hydrolyse polysaccharides such as Avicel and carboxymethyl-cellulose (Kusuda et al 2006).…”

Section: Ecological Relevance Of Secreted Enzymes For Em Fungimentioning

confidence: 99%

Saprotrophic capabilities as functional traits to study functional diversity and resilience of ectomycorrhizal community

Cullings

Courty

2009

Oecologia

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In an accompanying editorial Dr Petr Baldrian made a case casting doubt on our recent work addressing the saprophytic potential of ectomycorrhizal (EM) fungi. Dr Baldrian's statements illustrate a very valid truth: the book is still very much open on this subject. The point he raised that the only logical reason for these fungi to be responding to high carbon demand or decreased host photosynthetic capacity by up-regulating enzymes is for the purpose of carbon acquisition is valid as well. Despite this, he makes the case that there is no compelling evidence that EM fungi exhibit saprophytic activity. The concept central to Dr Baldrian's conclusion is that even though some EM fungi possess the genes necessary for saprophytic behaviour and may even express these genes, EM fungi do not inhabit a position in the soil column that provides access to usable substrate. In this paper we present both previously published and newly obtained data that demonstrate that this assumption is erroneous, and we present arguments that place the saprophytic potential of EM fungi within a broad ecological context.

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Detection of β-glucosidase as saprotrophic ability from an ectomycorrhizal mushroom, Tricholoma matsutake

Cited by 29 publications

References 13 publications

Tricholoma matsutake Dominates Diverse Microbial Communities in Different Forest Soils

Tricholoma matsutake Dominates Diverse Microbial Communities in Different Forest Soils

Effects of carbohydrate substrate on the vegetative mycelial growth of an ectomycorrhizal mushroom, Tricholoma matsutake, isolated from Quercus

Saprotrophic capabilities as functional traits to study functional diversity and resilience of ectomycorrhizal community

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