Detection, purification and efficacy of warnerin produced by Staphylococcus warneri

Prema, P.; Bharathy, Sukumar; Palavesam, A.; Sivasubramanian, Muthusaravanan; Immanuel, G.

doi:10.1007/s11274-005-9116-y

Cited by 15 publications

(7 citation statements)

References 15 publications

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“…However, the present result disaccords with our previous finding that a greater collection of culturable L. pneumophila in DW than TM (Chang and Chou, 2011a;Chang et al, 2010). Different susceptibilities of bacteria to 0.01% of Tween 80 may be one of the explanations for this inconsistency as the literature shows Tween 80 should be limited to 0.001% to avoid its interference on Legionella growth (Mondello et al, 2009), whereas Tween 80 at ≥0.1% facilitates Staphylococcus growth and its enzyme activity (Gould et al, 2009;Prema et al, 2006).…”

Section: Discussioncontrasting

confidence: 55%

“…This finding is probably attributed to the peptone and Tween 80 in TM. The peptone, including a variety of polypeptides, has been identified as the pivotal nutrients for S. aureus growth (Gray et al, 2008), while the Tween 80 (C 64 H 124 O 26 ) can be degraded by the lipase of S. aureus (Gould et al, 2009) and has been demonstrated to support the growth of Staphylococcus warneri (Prema et al, 2006). Interestingly, such beneficial effects of TM occurred not only during the sample storage but also at the air sampling, evident by increased airborne cell concentrations after 6-and 15-min sampling into TM of AGI-30 even though the cell counts in the nebulizer were continuously decreased (Figure 3b) and 16.6% of TM was lost at the end of 15-min sampling (Table 1).…”

Section: Discussionmentioning

confidence: 99%

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Methods for quantifyingStaphylococcus aureusin indoor air

Chang

Wang

2014

Indoor Air

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Staphylococcus aureus has been detected in indoor air and linked to human infection. Quantifying S. aureus by efficient sampling methods followed by appropriate sample storage treatments is essential to characterize the exposure risk of humans. This laboratory study evaluated the effects of sampler type (all-glass impinger (AGI-30), BioSampler, and Andersen one-stage sampler (Andersen 1-STG)), collection fluid (deionized water (DW), phosphate-buffered saline (PBS), and Tween mixture (TM)), and sampling time (3-60 min) on cell recovery. Effects of storage settings on bacterial concentration were also assessed over 48 h. Results showed BioSampler performed better than Andersen 1-STG and AGI-30 (P < 0.05) and TM was superior to PBS and DW (P < 0.05). An increase in sampling time negatively affected the recoveries of cells in PBS of BioSampler and AGI-30 (P < 0.05), whereas cell recoveries in TM were increased at sampling of 6-15 min compared with 3 min. Concentrations of cells collected in PBS were decreased with storage time at 4 and 23 °C (P < 0.05), while cells stored in TM showed stable concentrations at 4 °C (P > 0.05) and increased cell counts at 23 °C (P < 0.05). Overall, sampling by BioSampler with TM followed by sample transportation and storage at 4 °C is recommended.

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Section: Discussioncontrasting

confidence: 55%

Section: Discussionmentioning

confidence: 99%

Methods for quantifyingStaphylococcus aureusin indoor air

Chang

Wang

2014

Indoor Air

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“…These results suggest that the optimum pH for maximum bacteriocin activity and total viable cell production is 6.5. The results are in accord with the production of the bacteriocins warnerin in S. warneri [15], thermophilin 1277 in S. thermophilus SBT1277 [16], bacteriocin in L. pentosus ST71BZ [17], antibacterial proteins in B. licheniformis AnBa9 [12] and antibacterial compounds in B. coagulans [18], in which high levels of bacteriocin and total viable cell production were achieved at a suboptimal temperature and pH. Based on these results, the optimum incubation temperature, culture pH for bacteriocin production and maximal viable cell count for E. faecium MC13 were determined to be at 35 o C and 6.5 ( Fig.…”

Section: Effect Of Temperature Ph Nacl On Bacteriocin and Total Viasupporting

confidence: 87%

The role of environmental factors and medium composition on bacteriocin production by an aquaculture probiotic Enterococcus faecium MC13 isolated from fish intestine

Kanmani

Satishkumar

Yuvaraj

et al. 2011

Korean J. Chem. Eng.

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The aim of this study was to optimize medium composition for higher yield of total viable cells and bacteriocin by Enterococcus faecium MC13. The factors such as peptone, meat extract, yeast extract, lactose, glycerol, tween 80, triammonium citrate and K 2 HPO 4 were selected based on the Lactobacillus MRS medium composition. Two level factorial designs (FD) and steepest ascent path were performed to identify vital factors among the variables. Through the 2 −8 FD, peptone, yeast extract and lactose were found to be significant factors involved in the enhanced production of viable cells and bacteriocin. Therefore, these three foremost factors were further optimized by central composite design to achieve efficient yield. The optimum MRS composition was found to be peptone (40.0 g/L), meat extract (30.0 g/L), yeast extract (40.0 g/L), lactose (24.0 g/L), glycerol (5.8 g/L), Tween 80 (3.0 g/L), triammonium citrate (1.0 g/L), K 2 HPO 4 (2.5 g/L), MgSO 4 ·7H 2 O (0.10 g/L), MnSO 4 ·7H 2 O (0.05 g/L) and dipotassium PO 4 (2.0 g/L). The optimized growth medium allowed higher amount of bacteriocin activity (36,100 AUml −1 ) and total viable cells (14.22 LogCFUml −1 ) production which were two-times higher than the commercial MRS medium.

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“…In . In total, the literature on the inhibitory spectrum of staphylococcins indicates their efficiency against B. cereus and L. monocytogenes (Villani et al, 1997;Carnio et al, 2000;dos Santos Nascimento et al, 2005;Prema et al, 2006;Saeed et al, 2006;Ceotto et al, 2009Ceotto et al, , 2010aBrito et al, 2011;Fagundes et al, 2011;de Souza Duarte et al, 2013;Fagundes et al, 2016). Compared with the partially purified staphylococcin from Staph.…”

Section: Discussionmentioning

confidence: 99%

“…However, a less antilisterial action has been reported for warnerin, produced by Staph. warneri (Prema et al, 2006), and aureocin A70, produced by Staph. aureus A70 (inhibition zone diameters of 1.75 to 2.05 cm; Fagundes et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial spectrum activity of bacteriocinogenic Staphylococcus strains isolated from goat and sheep milk

Rahmdel

Shekarforoush

Hosseinzadeh

et al. 2019

Journal of Dairy Science

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Bacteriocins have attracted great attention as potential alternatives to antibiotics and chemical food additives. In the present study, 243 Staphylococcus isolates from milk samples (n = 110) of goat and sheep herds located in Fars province, Iran, were screened for antimicrobial substance production. Twenty-eight isolates showed an antagonistic activity against the indicator strain Micrococcus luteus ATCC 4698. The susceptibility of all antimicrobial substances to proteolytic enzymes allowed us to consider them as bacteriocin-like inhibitory substances (BLIS). The term BLIS is applied to uncharacterized proteinaceous antimicrobials produced by gram-positive bacteria. Based on molecular identification methods, the isolates belonged to the species Staphylococcus chromogenes, Staphylococcus epidermidis, Staphylococcus haemolyticus, Staphylococcus pseudintermedius, Staphylococcus aureus, and Staphylococcus agnetis. Pulsed-field gel electrophoresis revealed a high level of genotype diversity among the Staph. chromogenes isolates. All of the isolates harbored nukA or bsaA2 genes, suggesting that their BLIS were related to nukacin or Bsa. The antimicrobial compounds from test strains were not effective against gram-negative pathogens, including Salmonella enterica serovar Typhimurium, Escherichia coli O157:H7, and Klebsiella pneumonia as well as the indicator mold Aspergillus fumigatus. All the gram-positive targets, including Bacillus cereus, Listeria monocytogenes, Enterococcus faecalis Ef37 (a tyramine-producer strain), Lactobacillus saerimneri 30a (a histamine-producer strain), and methicillin-resistant Staph. epidermidis, were inhibited by the Staph. chromogenes isolates. Staphylococcus haemolyticus 4S12 was able to inhibit the majority of gram-positive bacteria. Listeria monocytogenes strains were the only indicators sensitive to the antimicrobial agents produced by Staph. agnetis 4S97B. The other Staphylococcus strains were ineffective on all the organisms tested. Based on their inhibitory capacities, the BLIS produced by the Staph. chromogenes isolates seem to be interesting candidates for developing novel antimicrobial agents.

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Detection, purification and efficacy of warnerin produced by Staphylococcus warneri

Cited by 15 publications

References 15 publications

Methods for quantifyingStaphylococcus aureusin indoor air

Methods for quantifyingStaphylococcus aureusin indoor air

The role of environmental factors and medium composition on bacteriocin production by an aquaculture probiotic Enterococcus faecium MC13 isolated from fish intestine

Antimicrobial spectrum activity of bacteriocinogenic Staphylococcus strains isolated from goat and sheep milk

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