Deteksi Virus pada Bawang Merah (Allium cepa var. ascalonicum) dengan Metode Dot Immuno Binding Assay

Wulandari, Astri; Hidayat, Sri Hendrastuti; Sobir, Sobir

doi:10.21082/jhort.v25n4.2015.p350-356

Cited by 12 publications

(13 citation statements)

References 2 publications

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“…The seed bulbs used in Enrekang are cv Katumi which were purchased from traders in Nganjuk, while farmers in Brebes and Probolinggo planted cv Bima and cv Biru Lancur which were produced by themselves. It was reported before that virus infection was very high in Enrekang, Brebes, and Probolinggo (Gunaeni et al 2011;Kurniawan and Suastika 2013;Swari et al 2015;Wulandari et al 2015).…”

Section: Virus Detection Based On Dibamentioning

confidence: 88%

“…Infection of Potyviruses (OYDV/Onion yellow dwarf virus, SYSV/Shallot yellow stripe virus, and LYSV/Leek yellow stripe virus) and Carlaviruses (SLV/Shallot latent virus dan GarCLV/Garlic common latent virus) has been reported previously, and the incidence reached 11.2% to 78% (Sutarya et al 1993;Arisuryanti et al 2008;Kadwati and Hidayat 2015;Swari et al 2015;Gunaeni et al 2018). Among those viruses, infection of OYDV was always recorded very high and becomes a major concern for several regions (Sutarya et al 1993;Bagi et al 2012;Kadwati and Hidayat 2015;Wulandari et al 2015;Gunaeni et al 2018;Saputri et al 2018).…”

Section: Introductionmentioning

confidence: 99%

“…Shallot viruses, including OYDV, SYSV, SLV, and GarCLV have been detected from bulbs collected from Java island, i.e. East Java (Brebes) and West Java (Cirebon, Majalengka, Kuningan and Bandung) (Wulandari et al 2015;Saputri et al 2018). The list is hypothetical will increase since the growing area of shallot production has spread widely in Indonesia.…”

Section: Introductionmentioning

confidence: 99%

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Detection of major viruses infecting shallot and molecular characterization of Onion yellow dwarf virus from several locations in Indonesia

et al. 2020

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Abstract. Harti H, Hidayat SH, Sobir, Wiyono S. 2020. Detection of major viruses infecting shallot and molecular characterization of Onion yellow dwarf virus from several locations in Indonesia. Biodiversitas 21: 1697-1701. Research was conducted to identify main viruses infecting shallot in several regions in Indonesia and to further characterize genetic variation of Onion yellow dwarf virus (OYDV). Field survey was conducted in Central Java (Brebes), East Java (Probolinggo), West Sumatera (Alahan Panjang), West Nusa Tenggara (Bima), and South Sulawesi (Enrekang). Virus detection from field samples was conducted by dot immunobinding assay. This detection confirmed that infection of OYDV, Shallot yellow stunt virus (SYSV), Shallot latent virus (SLV), and Garlic common latent virus (GarCLV) has occurred in all field with incidence ranged from 20 to 93.5%, 2 to 93%, 21.5 to 80%, and 2 to 80.5%, respectively. The specific primers of Nib gene successfully amplified DNA fragments of OYDV from all locations. Sequencing of DNA fragments revealed that the amplified product was 351 bp. Sequence analysis indicated that the present OYDV isolates from Indonesia shared homology from 82 to 95%; and they had homology from 81 to 95% with OYDV isolates from other countries. The similarity of OYDV isolates from different geographical locations reflected the movements of seed bulbs among and within countries. The phylogenetic tree also revealed that OYDV isolates from different countries did not group together indicating their diverse origin.

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Section: Virus Detection Based On Dibamentioning

confidence: 88%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Detection of major viruses infecting shallot and molecular characterization of Onion yellow dwarf virus from several locations in Indonesia

et al. 2020

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“…Selain F. oxysporum, infeksi virus juga banyak ditemukan pada pertanaman bawang merah walaupun belum dianggap sebagai masalah penting oleh petani bawang merah di Indonesia. Infeksi Onion yellow dwarf virus (OYDV), Shallot yellow stripe virus (SYSV), Garlic common latent virus (GCLV) pada beberapa kultivar bawang merah di Indonesia dilaporkan oleh Gunaeni et al (2011) dan Wulandari et al (2015) dan berpotensi menyebabkan kehilangan hasil.…”

Section: Pendahuluanunclassified

Insidensi Virus dan Cendawan pada Biji dan Umbi Bawang Merah

Saputri

Tondok

Hidayat

2019

J Fitopatol Indones

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Incidence of Viruses and Fungi on True Shallot Seed and Shallot Bulb Shallot is an important vegetable in Indonesia. Shallot farmers generally use bulbs as planting material even though there is an option to use true shallot seed (TSS). One important criteria for a good seed quality as planting material is pathogen free. Research was conducted to detect and identify viruses and fungi from TSS and shallot seed bulbs cultivars Bima, Bauji, Thailand, and Tuk-Tuk. Virus detection was carried out by DIBA (dot immunobinding assay) method using specific antiserum to OYDV, SLV, SYSV, and GCLV. Detection and identification of fungi was carried out by blotter test method and polymerase chain reaction. Infection of OYDV, GCLV, SYSV, and SLV were only detected in shallot bulbs with infection rates ranging from 66% to 100%. Four species of fungi were detected in TSS and bulbs, i.e. Aspergillus niger, A.flavus, F. solani, and Rhizopus sp; whereas F.oxysporum was only found in bulbs. All isolates of F. oxysporum was pathogenic and cause disease incidence up to 55%. Pathogenic isolate of F. oxysporum had 100% homology to those isolate from China and USA on the spesies level and 91.2% to those isolate from India and USA to the forma spesies level.

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“…Baru-baru ini Kadwati dan Hidayat (2015) melaporkan bahwa rata-rata infeksi virus pada bawang merah di lahan berkisar 11.2% sampai 14.3%, sedangkan pada sampel umbi berkisar 11.2% sampai 13.3%. Wulandari et al (2015) juga mendeteksi infeksi OYDV, SLV, dan GarCLV yang sangat tinggi pada bawang merah, yaitu berturut-turut 97.78%, 92.98%, dan 92.18%. Pengaruh infeksi virus terhadap kehilangan hasil bawang merah di Indonesia belum banyak diketahui.…”

Section: Pendahuluanunclassified

Hot Water Treatment on Shallot (Allium cepa var. ascalonicum) Tuber to Suppress Viruses Infection in The Field

et al. 2019

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High infestation of viruses on shallot's bulb has been reported, although little is known on the effect of virus infection on shallot productivity. The use of virus-free bulbs is assumed to be the key factor to improve productivity. Hot water treatment of bulbs before planting is one of methodologies to eliminate virus from shallot bulbs. Therefore, research was conducted to study the effectiveness of hot water treatment methods of shallots bulbs to reduce virus infections in the field. Field experiment was conducted using split plot randomized block design with two factors. The first factor was the use of netting, i.e. growing shallot in netting house and in open field. The second factor was hot water treatment of shallot bulb at 45 0 C for 15, 30, and 45 min and without treatment. Observations were conducted on the incidence of virus infections, plant growth (number of tillers and plant height) and shallots productivity. Virus infection was confirmed using specific antibodies. Observation of disease symptom indicated that the use of netting house did not significantly suppress the incidence of virus diseases, while hot water treatment significantly reduced the incidence of virus diseases. Hot water treatment for 15, 30 and 45 minutes at 45 0 C was able to suppress virus incidence in the field up to 54.98%, 56.77% and 64.35%, respectively. ABSTRAKInfestasi virus pada umbi bawang merah dilaporkan sangat tinggi, meskipun efek infeksi virus terhadap produktivitas bawang merah masih sedikit diketahui. Penggunaan umbi bebas virus diasumsikan menjadi salah satu faktor utama untuk meningkatkan produktivitas. Perlakuan air panas pada umbi sebelum tanam merupakan metode pilihan untuk mengeliminasi virus. Penelitian dilakukan dengan tujuan mempelajari keefektifan metode perlakuan air panas pada umbi bibit bawang merah terhadap infeksi virus di lapangan. Penelitian disusun menggunakan rancangan acak lengkap petak terbagi dengan dua faktor. Faktor pertama adalah perlakuan rumah kasa dengan dua taraf, yaitu penanaman dalam rumah kasa dan penanaman di lahan terbuka. Faktor kedua adalah perlakuan air panas suhu 45 0 C dengan 4 taraf waktu perendaman, yaitu 15 menit, 30 menit, 45 menit dan kontrol (tanpa perlakuan). Pengamatan dilakukan terhadap insidensi penyakit, parameter pertumbuhan tanaman (jumlah anakan dan tinggi tanaman), dan produktivitas tanaman. Insidensi virus dikonfirmasi dengan deteksi menggunakan antibodi spesifik. Hasil pengamatan gejala menunjukkan bahwa perlakuan rumah kasa tidak berpengaruh nyata terhadap penekanan insidensi penyakit, sementara perlakuan pemanasan berpengaruh nyata terhadap penekanan insidensi penyakit. Waktu perendaman umbi selama 15, 30 dan 45 menit pada suhu 45 0 C dapat menekan insidensi penyakit virus dilapangan berturut-turut sebesar 54.98%, 56.77% dan 64.35%.Kata kunci: eliminasi virus, insidensi penyakit, rumah kasa, waktu perendaman

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Deteksi Virus pada Bawang Merah (Allium cepa var. ascalonicum) dengan Metode Dot Immuno Binding Assay

Cited by 12 publications

References 2 publications

Detection of major viruses infecting shallot and molecular characterization of Onion yellow dwarf virus from several locations in Indonesia

Detection of major viruses infecting shallot and molecular characterization of Onion yellow dwarf virus from several locations in Indonesia

Insidensi Virus dan Cendawan pada Biji dan Umbi Bawang Merah

Hot Water Treatment on Shallot (Allium cepa var. ascalonicum) Tuber to Suppress Viruses Infection in The Field

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