Determinants of AhR-mediated transcriptional activity induced by plasma extracts from Nunavik Inuit adults

Medehouenou, Thierry Comlan Marc; Larochelle, Christian; Dumas, Pierre; Dewailly, Éric; Ayotte, Pierre

doi:10.1016/j.chemosphere.2010.04.017

Cited by 17 publications

(17 citation statements)

References 29 publications

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“…A semi-automated solid-phase extraction method was used to prepare purified extracts for AhR-mediated transcriptional activity analyses from a single 5-ml plasma sample as described in Medehouenou et al [54]. In short, the plasma sample was mixed with equal parts of formic acid and deionised water and extracted using a methanol: dichloromethane mixture (1/9).…”

Section: Methodsmentioning

confidence: 99%

“…Then the extract was cleaned up on activated silica/acidic silica column and the compounds were eluted with dichloromethane. The solvent was evaporated to dryness and the resulting fraction reconstituted in 5 μl of dimethylsulfoxide for the measurement of AhR-mediated transcriptional activity [54]. …”

Section: Methodsmentioning

confidence: 99%

“…Detail procedure of AhR-mediated transactivity measurement was described elsewhere [54,55]. In short, the bioassay used to measure the AhR mediated transactivity was based on the expression of the firefly luciferase in H4IIE.Luc cells resulting from the activation of the AhR pathway by AhR activating compounds.…”

Section: Methodsmentioning

confidence: 99%

“…AhR-mediated transcriptional activity of cleaned plasma extracts was interpolated onto the TCDD dose-response curve of AhR mediated luciferase activity and expressed as TCDD equivalents (AhR-TEQ), which represents the total TCDD-toxic potency of a mixture of dioxin-like compounds. The limit of detection was 30 pg TEQ/L, corresponding to approximately 5 pg TEQ/g lipids [54]. Measurement of AhR-mediated transactivity was performed at the Centre de Toxicologie (CTQ) of the Institut National de Sante Publique du Quebec (Quebec, Canada).…”

Section: Methodsmentioning

confidence: 99%

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Perfluorinated compounds are related to breast cancer risk in greenlandic inuit: A case control study

et al. 2011

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BackgroundBreast cancer (BC) is the most common cancer for women in the western world. From very few cases an extraordinary increase in BC was observed in the Inuit population of Greenland and Canada although still lower than in western populations. Previous data suggest that exposure to persistent organic pollutants (POPs) might contribute to the risk of BC. Rat studies showed that perfluorinated compounds (PFCs) cause significantly increase in mammary fibroadenomas. This study aimed at evaluating the association between serum levels of POPs/PFCs in Greenlandic Inuit BC cases and their controls, and whether the combined POP related effect on nuclear hormone receptors affect BC risk.MethodsThirty-one BC cases and 115 controls were sampled during 2000-2003 from various Greenlandic districts. The serum levels of POPs, PFCs, some metals and the combined serum POP related effect on estrogen- (ER), androgen- (AR) and Ah-receptor (AhR) transactivity were determined. Independent student t-test was used to compare the differences and the odds ratios were estimated by unconditional logistic regression models.ResultsWe observed for the very first time a significant association between serum PFC levels and the risk of BC. The BC cases also showed a significantly higher concentration of polychlorinated biphenyls at the highest quartile. Also for the combined serum POP induced agonistic AR transactivity significant association to BC risk was found, and cases elicited a higher frequency of samples with significant POP related hormone-like agonistic ER transactivity. The AhR toxic equivalent was lowest in cases.ConclusionsThe level of serum POPs, particularly PFCs, might be risk factors in the development of BC in Inuit. Hormone disruption by the combined serum POP related xenoestrogenic and xenoandrogenic activities may contribute to the risk of developing breast cancer in Inuit. Further investigations are needed to document these study conclusions.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Perfluorinated compounds are related to breast cancer risk in greenlandic inuit: A case control study

et al. 2011

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“…PCB153 concentration (Pearson's r=0.53, p<0.001), age and n-3 fatty acids in erythrocyte membranes (p<0.001) correlated positively with AhR-mediated activity, but negatively for body fat mass (p=0.037). The AhR-mediated transcriptional activity was suggested as linked to plasma OC body burden, dioxin-like PCBs, polychlorinated dibenzo- p -dioxins and polychlorodibenzofurans (104). …”

Section: Determinants and Effects Of Ahr Functionmentioning

confidence: 99%

Health effects associated with measured levels of contaminants in the Arctic

Weihe

Debes

Halling

et al. 2016

International Journal of Circumpolar Health

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The Human Health Assessment Group has over the past decade recommended that effect studies be conducted in the circumpolar area. Such studies examine the association between contaminant exposure in the Arctic populations and health effects. Because foetuses and young children are the most vulnerable, effect studies are often prospective child cohort studies. The emphasis in this article is on a description of the effects associated with contaminant exposure in the Arctic. The main topics addressed are neurobehavioural, immunological, reproductive, cardiovascular, endocrine and carcinogenic effect. For each topic, the association between exposure and effects is described, and some results are reported for similar studies outside the Arctic.

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Persistent Organic Pollutants, Mitochondrial Dysfunction, and Metabolic Syndrome

Lee

Pak

2018

Mitochondrial Dysfunction Caused by Drugs and Environmental Toxicants

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Determinants of AhR-mediated transcriptional activity induced by plasma extracts from Nunavik Inuit adults

Cited by 17 publications

References 29 publications

Perfluorinated compounds are related to breast cancer risk in greenlandic inuit: A case control study

Perfluorinated compounds are related to breast cancer risk in greenlandic inuit: A case control study

Health effects associated with measured levels of contaminants in the Arctic

Persistent Organic Pollutants, Mitochondrial Dysfunction, and Metabolic Syndrome

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