Determinants of DNA yield and purity collected with buccal cell samples

Wijer, Diane B M A Van Wieren-De; Zee, Anke‐Hilse Maitland‐van der; Boer, Anthonius de; Belitser, Svetlana V.; Kroon, Abraham A.; Leeuw, Peter W. de; Schiffers, P. M. H.; Janssen, Rob; Duijn, Cornelia M. van; Stricker, Bruno H.; Klungel, Olaf H.

doi:10.1007/s10654-009-9388-x

Cited by 30 publications

(19 citation statements)

References 19 publications

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“…Most GWAS analyses are strongly embedded in the recently started Early Growth Genetics (EGG) Consortium and Early Genetics and Longitudinal Epidemiology (EAGLE) Consortium, in which several birth cohort studies combine their GWAS efforts focused on multiple outcomes in fetal life, childhood and adolescence [160,161]. The power of large scale consortia has been shown previously [162][163][164][165][166][167][168][169][170]. Also, strategies for genetic scoring analyses are being developed to estimate predictive values of identified genetic variants [171,172].…”

Section: Biological Samplesmentioning

confidence: 99%

The Generation R Study: design and cohort update 2012

et al. 2012

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The Generation R Study is a population-based prospective cohort study from fetal life until young adulthood. The study is designed to identify early environmental and genetic causes of normal and abnormal growth, development and health during fetal life, childhood and adulthood. The study focuses on four primary areas of research: (1) growth and physical development; (2) behavioural and cognitive development; (3) diseases in childhood; and (4) health and healthcare for pregnant women and children. In total, 9,778 mothers with a delivery date from April 2002 until January 2006 were enrolled in the study. General follow-up rates until the age of 4 years exceed 75%. Data collection in mothers, fathers and preschool children included questionnaires, detailed physical and ultrasound examinations, behavioural observations, and biological samples. A genome wide association screen is available in the participating children. Regular detailed hands on assessment are performed from the age of 5 years onwards. Eventually, results forthcoming from the Generation R Study have to contribute to the development of strategies for optimizing health and healthcare for pregnant women and children.

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Section: Biological Samplesmentioning

confidence: 99%

The Generation R Study: design and cohort update 2012

et al. 2012

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“…By then, it can also serve as a GWAs dataset for both SNP frequencies and copy number variations (CNVs) in Chinese Han population. Additional publications about genetic and biomarker studies and loco-motor diseases in EJE can be found in [111][112][113][114][115][116][117][118][119][120][121]. …”

Section: Genome Wide Association Studiesmentioning

confidence: 99%

The Shanghai Changfeng Study: a community-based prospective cohort study of chronic diseases among middle-aged and elderly: objectives and design

Gao

Hofman

et al. 2010

Eur J Epidemiol

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The Shanghai Changfeng Study is a community-based prospective cohort study of chronic diseases ongoing since February 2009 in Shanghai, China. The study focuses on multiple chronic diseases, including obesity and metabolic syndrome, diabetes, osteoporosis, liver diseases, cardiovascular diseases and neurologic diseases. 15,000 subjects of 40 years or over are planned to be recruited. The rationale, objectives and design of this study are described in this paper.

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“…2 Buccal cell collection can be performed easily by a buccal swab with a cotton swab or using a mouth-wash procedure. 3 DNA isolation using buccal swabs provides many advantages, such as cost-effective processing, lower sample volume requirement, long-term archiving, and suitability of self-collection. It is more comfortable for the patient, and the buccal swabs provide sufficient DNA for the PCRs, as they demands only a few nanograms of DNA.…”

Section: Introductionmentioning

confidence: 99%

“…It is more comfortable for the patient, and the buccal swabs provide sufficient DNA for the PCRs, as they demands only a few nanograms of DNA. 3 Human hair is one of the most common biological materials associated with legal investigations and has been used for the statistics-based population work and DNAbased analysis in criminology. 4 The most valuable method of DNA testing is short tandem repeat analysis of nuclear DNA.…”

Section: Introductionmentioning

confidence: 99%

A Simple Method of Genomic DNA Extraction from Human Samples for PCR-RFLP Analysis

Ghatak¹,

Muthukumaran

Kumar³

2013

J Biomol Tech

137

102

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Isolation of DNA from blood and buccal swabs in adequate quantities is an integral part of forensic research and analysis. The present study was performed to determine the quality and the quantity of DNA extracted from four commonly available samples and to estimate the time duration of the ensuing PCR amplification. Here, we demonstrate that hair and urine samples can also become an alternate source for reliably obtaining a small quantity of PCR-ready DNA. We developed a rapid, cost-effective, and noninvasive method of sample collection and simple DNA extraction from buccal swabs, urine, and hair using the phenol-chloroform method. Buccal samples were subjected to DNA extraction, immediately or after refrigeration (4 -6°C) for 3 days. The purity and the concentration of the extracted DNA were determined spectrophotometerically, and the adequacy of DNA extracts for the PCR-based assay was assessed by amplifying a 1030-bp region of the mitochondrial D-loop. Although DNA from all the samples was suitable for PCR, the blood and hair samples provided a good quality DNA for restriction analysis of the PCR product compared with the buccal swab and urine samples. In the present study, hair samples proved to be a good source of genomic DNA for PCR-based methods. Hence, DNA of hair samples can also be used for the genomic disorder analysis in addition to the forensic analysis as a result of the ease of sample collection in a noninvasive manner, lower sample volume requirements, and good storage capability.

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Determinants of DNA yield and purity collected with buccal cell samples

Cited by 30 publications

References 19 publications

The Generation R Study: design and cohort update 2012

The Generation R Study: design and cohort update 2012

The Shanghai Changfeng Study: a community-based prospective cohort study of chronic diseases among middle-aged and elderly: objectives and design

A Simple Method of Genomic DNA Extraction from Human Samples for PCR-RFLP Analysis

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